Samples of commercial cover brines were collected as described above and spun at 12000 rpm for 10 min at 21 °C (ambient temperature) to remove residual particulate matter (Eppendorf Benchtop Refrigerated Centrifuge 5810R, Hamburg, Germany). Samples were diluted 10X prior to analysis with distilled water. Organic acids and sugars concentrations were measured using a 30-cm HPX-87H column (Bio-Rad) (McFeeters and Barish 2003 (link)). The column was heated to 37 °C and eluted with 0.03N sulfuric acid at a flow rate of 0.6 mL/min. A Thermo Separations UV6000 diode array detector (Spectra System Thermo Fisher Scientific Inc.) set to collect data at 210 nm was used to detect malic, lactic, acetic, propionic, and butyric acids. A Waters model 410 refractive index detector (Waters Corp., Millipore Corp., Billerica, Mass., U.S.A.) connected in series with the diode array detector was used to measure glucose, fructose, and ethanol. External standardization of the detectors was done using four concentrations of the standard compounds.
30 cm hpx 87h column
Manufactured by Bio-Rad
Sourced in United States
The 30-cm HPX-87H column is a chromatography column used for the separation and analysis of carbohydrates, organic acids, and other small molecules. It is designed to operate at high performance liquid chromatography (HPLC) conditions.
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Lab products found in correlation
3 protocols using 30 cm hpx 87h column
1
Quantifying Organic Acids and Sugars in Brines
2
HPLC Analysis of Metabolite Concentrations
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The concentrations of metabolites and substrates were measured by HPLC using a 30 cm HPX-87H column (Bio-Rad Laboratories, Hercules, CA, USA) for component separation. The column temperature was maintained at 37 °C, and the elution of components was performed with 0.03 N sulfuric acid at a flow rate of 0.6 mL/min. A Thermo Separations UV6000 diode array detector (Spectra System Thermo Scientific, Waltham, MA, USA) and a Waters model 410 refractive index detector (Waters Corp., Millipore Corp., Billerica, MA, USA), connected in series with the diode array detector, were used to measure glucose, fructose, ethanol, and other metabolites. External standardization of the detectors was performed using four concentrations of standard compounds [23 (link)].
3
Quantifying Lactic Acid and pH
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Lactic acid concentration and pH were measured as described by Pérez-Díaz et al. (2019) (link). Briefly, lactic acid concentration was measured by high-performance liquid chromatography analysis using a 30-cm HPX-87H column (Bio-Rad Laboratories, Hercules, CA, United States) as described by McFeeters and Barish (2003) (link). The column was heated to 37°C and eluted with 0.03N sulfuric acid at a flow rate of 1 ml/min. A Thermo Separations UV6000 diode array detector (Spectra System Thermo Scientific, Waltham, MA, United States) was used to collect data at 210 nm for the analysis of lactic acid. External standards were used to calibrate the system. The pH of each cover brine sample was measured using a Fisher Accumet pH meter (model AR25, Fisher Scientific).
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