We purchased JNK inhibitor SP600125 from Cayman Chemical (Ann Arbor, MI, USA), and transforming growth factor alpha (TGF‐α) from R&D Systems (Minneapolis, MN, USA). Primary antibodies used in immunohistochemical and immunofluorescence assays included rabbit anti‐phospho‐JNK antibody (1:50; Cell Signaling Technology, Danvers, MA, USA), rat anti‐mouse CD45 antibody (1:50; BD Biosciences, San Jose, CA, USA), rat anti‐mouse F4/80 antibody (1:100; eBioscience, San Diego, CA, USA), rabbit anti‐Ki‐67 antibody (1:100; Abcam, Cambridge, UK), mouse anti‐α‐smooth muscle actin (α‐SMA) antibody (1:50; Santa Cruz, Santa Cruz, CA, USA), biotin hamster anti‐mouse CD11c antibody (1:100; BD Biosciences), rabbit anti‐cleaved caspase3 antibody (1:1600; Cell Signaling Technology) and rabbit anti‐CD8 antibody (clone EP1150Y, 1:250; Epitomics, Burlingame, CA, USA). Primary antibodies used in immunoblotting analysis included rabbit anti‐phospho‐JNK antibody (1:1000; Cell Signaling Technology), rabbit anti‐JNK antibody (1:1000; Cell Signaling Technology), rabbit anti‐phospho‐Stat3 antibody (1:2000; Cell Signaling Technology) and mouse anti‐Stat3 antibody (1:1000; Cell Signaling Technology).
Mouse anti stat3 antibody
Manufactured by Cell Signaling Technology
Sourced in China
The Mouse anti-Stat3 antibody is a specific antibody that recognizes the Stat3 protein in mouse samples. Stat3 is a transcription factor that plays a crucial role in various cellular processes, including cell growth, differentiation, and survival. This antibody can be used for the detection and analysis of Stat3 in mouse-derived samples, such as cell lysates or tissue extracts, through techniques like Western blotting or immunohistochemistry.
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Lab products found in correlation
Rabbit anti cleaved caspase 3 antibody, by Cell Signaling Technology (1 mentions)
Jnk inhibitor sp 600125, by Cayman Chemical (1 mentions)
Tgf α, by R&D Systems (1 mentions)
Rabbit anti phospho jnk antibody, by Cell Signaling Technology (1 mentions)
Rat anti mouse cd45 antibody, by BD (1 mentions)
Rat anti mouse f4 80 antibody, by Thermo Fisher Scientific (1 mentions)
Rabbit anti jnk antibody, by Cell Signaling Technology (1 mentions)
Rabbit anti phospho stat3 antibody, by Cell Signaling Technology (1 mentions)
Rabbit anti ki67 antibody, by Abcam (1 mentions)
Alexa fluor 568 conjugated goat anti mouse secondary antibody, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 conjugated donkey anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions)
Vectashield mounting medium, by Vector Laboratories (1 mentions)
Bx51 fluorescence microscope, by Olympus (1 mentions)
Rabbit anti flag antibody, by Cell Signaling Technology (1 mentions)
Mouse anti α tubulin antibody, by Santa Cruz Biotechnology (1 mentions)
Mouse anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti akt antibody, by Cell Signaling Technology (1 mentions)
Anti p stat3 rabbit antibody, by Cell Signaling Technology (1 mentions)
Ventana dabmap detection kit, by Roche (1 mentions)
Anti ezrin rabbit antibody, by Cell Signaling Technology (1 mentions)
4 protocols using mouse anti stat3 antibody
1
Inhibition of JNK Signaling in Immune Cells
2
Immunofluorescence Staining of STAT3 and GRN
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Cells grown on glass cover slips were fixed with 4% paraformaldehyde/PBS for 20 min, permeabilized with 0.5% Triton X-100/H2O for 30 min, and blocked in 5% bovine serum albumin/PBS for 1 hour at room temperature. Cells were costained with a mouse antiSTAT3 antibody (1:500 dilution, 9139; Cell Signaling) and a rabbit anti-GRN antibody (1:100 dilution, 40-3400; Invitrogen) overnight at 4C, followed by Alexa Fluor 568-conjugated goat anti-mouse secondary antibody (1:500 dilution, A-11004; Life Technologies) and Alexa Fluor 488-conjugated donkey anti-rabbit secondary antibody (1:500 dilution, A-21206; Life Technologies) for 1 hour. Cover slips were mounted onto glass slides using DAPI-containing Vectashield mounting medium (H-1200; Vector Laboratories, Inc., Burlingame, CA). Images were acquired using an Olympus BX51 fluorescence microscope with attached DP71 camera and DP Manager Software. Three microscopic fields/slide were photographed (40x).
3
Comprehensive Western Blotting Analysis
4
Immunohistochemical Analysis of Signaling Proteins
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Sequential sections (3‐μm thick) were prepared from the paraffin blocks of maximum cut surface specimens for each case. These samples were immunohistochemically stained for ezrin, ERK, AKT, and STAT3. The intensity of staining was classified on a 4‐point scale: 0, 1+, 2+, and 3+, with 0 and 1+ indicating low levels and 2+ and 3+ indicating high levels. The degree of staining was compared between CIS and SCC. The antibodies used were anti‐ezrin rabbit antibody (1:100, #3145; Cell Signaling, Danvers, MA), anti‐p44/42 MAP kinase mouse antibody (1:500, #4696; Cell Signaling), anti‐AKT mouse antibody (1:250, #2920; Cell Signaling), and anti‐STAT3 mouse antibody (1:600, #9139; Cell Signaling.
Immunostaining was performed using a Discovery XT Automated IHC Stainer with a Ventana DABMap Detection kit (no. 760–124; Ventana Medical System, Oro Valley, AZ). Each step of the Ventana DABMap Detection kit procedure was optimized for the Discovery XT instrument; conditions including the dilutions of each antibody were established before measurements. In all cases, the antigen was activated by heating.
Immunostaining was performed using a Discovery XT Automated IHC Stainer with a Ventana DABMap Detection kit (no. 760–124; Ventana Medical System, Oro Valley, AZ). Each step of the Ventana DABMap Detection kit procedure was optimized for the Discovery XT instrument; conditions including the dilutions of each antibody were established before measurements. In all cases, the antigen was activated by heating.
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