Anti cd127 pe cy7

Manufactured by BD

Sourced in United States

Anti-CD127 Pe-Cy7 is a fluorescently labeled antibody that binds to the CD127 (IL-7 receptor alpha) cell surface marker. It is designed for use in flow cytometry applications to identify and characterize CD127-positive cells.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

CD127-PE (29 citations) Anti-CD127-PE (18 citations) CD127 PE-Cy7 (13 citations) Anti-CD127 (13 citations)

7 protocols using anti cd127 pe cy7

Multiparameter Flow Cytometry of T-cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Purified CD8⁺ T cells, splenocytes and thymocytes were first stained for surface antigens and then treated with Foxp3 staining buffer set according to the manufacturer's directions (eBioscience). Anti-Eomes AlexaFluor 647 or eFluor 660 (Dan11mag, 1/75), anti-T-bet PE (eBio4B10, 1/100) and anti-CD49d FITC or PE (R1-2, 1/50) antibodies were purchased from eBioscience. Anti-CD8 PercP (53–6.7, 1/50), anti-CXCR3 APC (CXCR3-173, 1/50), anti-CD4 Pe-Cy7 (RM4-5, 1/100), anti-CD62L PE (1/100) or V450 (1/50) (MEL-14), anti-Bcl2 PE (3F11, 1/25), anti-Ki67 FITC (B56, 1/25), anti-CD44 FITC or V450 (IM7, 1/50), anti-CD127 Pe-Cy7 (SB/199, 1/50), anti-CD122 FITC (TM-BETA1, 1/50), anti-NK1.1 FITC (PK136, 1/50), anti-CD90.2 Pe-Cy7 (53-2.1, 1/100) and anti-IFNγ APC or PB or PE (XMG1.21/50) were purchased from BD biosciences. Anti-CD3 Pe-Cy7 (2C11, 1/100) was purchased from Biolegend.
In some experiments, brefeldin A (5 μg ml⁻¹, Sigma) was added in samples for 3 h at 37 °C before intracytoplasmic staining. Blood samples were directly stained for surface antigens and then treated with FACS lysing buffer (BD biosciences) as described in the product data sheet. All samples were fixed with 1% paraformaldehyde in PBS prior to their processing using a Cyan flow cytometer (Dako Cytomation).

Martinet V., Tonon S., Torres D., Azouz A., Nguyen M., Kohler A., Flamand V., Mao C.A., Klein W.H., Leo O, & Goriely S. (2015). Type I interferons regulate eomesodermin expression and the development of unconventional memory CD8+ T cells. Nature Communications, 6, 7089.

+ Open protocol

+ Expand

Multi-parameter Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-CD127-PECy7, anti-CD25-PECy5, anti-CD45RA-APCH7, anti-CD19-FITC, and anti-CD3-APCCy7 were purchased from BD Biosciences (San Jose, CA, USA). Anti-CD4-PE and anti-CD8-PECy7 were purchased from Tonbo Biosciences (San Diego, CA, USA). Anti-FOXP3-AlexaFluor647 was purchased from Beckman Coulter (Brea, CA, USA). Carboxy fluorescein succinimidyl ester (CFSE) was purchased from Life Technologies (Eugene, OR, USA).
For intracellular staining, FOXP3/Transcription Factor Staining Buffer Set (eBiosciences, San Diego, CA, USA) was used. All staining were performed in RPMI 1640 (Gibco, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS), 2 mM glutamine, 10 mM HEPES, and 10 mM antibiotic-anti-mycotic (Gibco).

Alvarez Salazar E.K., Cortés-Hernández A., Alemán-Muench G.R., Alberú J., Rodríguez-Aguilera J.R., Recillas-Targa F., Chagoya de Sánchez V., Cuevas E., Mancilla-Urrea E., Pérez García M., Mondragón-Ramírez G., Vilatobá M., Bostock I., Hernández-Méndez E., De Rungs D., García-Zepeda E.A, & Soldevila G. (2017). Methylation of FOXP3 TSDR Underlies the Impaired Suppressive Function of Tregs from Long-term Belatacept-Treated Kidney Transplant Patients. Frontiers in Immunology, 8, 219.

+ Open protocol

+ Expand

CFSE-based Proliferation Assay of PBMCs from Active TB Patients

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMCs (5 × 10⁵cells/well) from active TB patients were thawed and rested 6 h before labeling with Carboxyfluorescein succinimidyl ester (CFSE) according to manufacturer’s procedure. In the COX-i treated samples, indomethacin was added 2 h prior to stimulation with ESAT-6 and Ag85. The samples were incubated for 6 days and then washed and stained for surface markers and viability staining. Fluorochromes used in the 6 days proliferation assay; anti-CD3-V450, anti-CD4- APC H7, anti-CD45RA -BV 605, anti-HLA DR- APC, anti-CD25-PE, anti-CD127- PeCy7 and 7AAD- PerCP (all from BD Bioscience) and CellTrace™ CFSE Cell Proliferation (Life technologies).

Tonby K., Wergeland I., Lieske N.V., Kvale D., Tasken K, & Dyrhol-Riise A.M. (2016). The COX- inhibitor indomethacin reduces Th1 effector and T regulatory cells in vitro in Mycobacterium tuberculosis infection. BMC Infectious Diseases, 16, 599.

+ Open protocol

+ Expand

Cytokine Production Assay for E7 Peptide

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells were incubated at a concentration of 3x10⁶ cells/well for 6 h at 37 ºC and 5% CO₂ with 10 µg/mL Brefeldin A (GolgiPlug; BD Biosciences), 5 ng/mL IL-2 (Thermo Fisher Scientific), and 300 ng/well of E7 peptide¹⁶ (amino acids 49-57; RAHYNIVTF; GenScript). After incubation, cells were stained for 30 min at 4 ºC with anti-CD8a-APC (BioLegend), anti-CD44-FITC (BioLegend), anti-CD62L-BV421 (BioLegend), anti-KLRG1-PE (BioLegend), and anti-CD127-PECy7 (BD Biosciences) antibodies. After fixation/permeabilization with the Cytofix/Cytoperm solution (BD Biosciences) for 10 min at 4 ºC, cells were stained with anti-IFN-γ antibody conjugated to Alexa700 (BD Biosciences) for 30 min at 4 ºC. The cells were then resuspended in PBS and examined by flow cytometry using the LSR Fortessa device (BD Biosciences).

Porchia B.F., Aps L.R., Moreno A.C., da Silva J.R., Silva M.D., Sales N.S., Alves R.P., Rocha C.R., Silva M.M., Rodrigues K.B., Barros T.B., Pagni R.L., Souza P.D., Diniz M.D, & Ferreira L.C. (2022). Active immunization combined with cisplatin confers enhanced therapeutic protection and prevents relapses of HPV-induced tumors at different anatomical sites. International Journal of Biological Sciences, 18(1), 15-29.

+ Open protocol

+ Expand

Isolation and Characterization of Skin Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The tissue-digesting enzyme solution containing 100 μg/mL DNase I (Solarbio, Beijing, China) and 1 mg/mL collagenase IV (Worthington, Lakewood, LA, USA) was prepared in a cold serum-free DMEM medium and kept on ice for later use. The skin tissue was cut into pieces less than 1 mm by sterile ophthalmology scissors and digested with digestive enzyme solution (about 5 mL of enzyme solution for 1 cm² of skin tissue) for 1.5–2 h in a steady-temperature incubator at 37 °C. Mouse skin tissue was filtered through a 70 µm cell strainer into PBS containing 2% FBS. The prepared cell suspension was directly used for staining and was fixed with 4% paraformaldehyde and stored at 4 °C.
Cell surface staining was performed with anti-CD45-BB515, anti-CD127-PE-CY7, anti-lineage-percp-cy5.5, and anti-ST2-BV421 (BD Pharmingen, California, USA), and incubated at 4 °C for 30 min in the dark. After washing with PBS, the cells were fixed with 4% paraformaldehyde and then permeabilized with 1× Perm/Wash Buffer (BD Pharmingen) for 15 min. Intracellular staining was performed with anti-GATA3-AF647 (BD Pharmingen). The cells were then incubated at 4 °C in the dark for 2 h and washed once with PBS. The data were acquired using a BD FACSCalibur flow cytometer (BD Bioscience, San Jose, CA) and analyzed with Flow Jo X.

Li Y., Lin S., Xiong S, & Xie Q. (2022). Recombinant Expression of Human IL-33 Protein and Its Effect on Skin Wound Healing in Diabetic Mice. Bioengineering, 9(12), 734.

+ Open protocol

+ Expand

Detailed Phenotyping of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-CD14 and anti-CD19 APC-H7 (both at 1:100), anti-CD25 PECy7 (1:50), anti-CD62L V450 (1:50), anti-CD127 PECy7 (1:100), anti-CD161 FITC (1:10), anti-IFNγ V450 (1:200), anti-Ki67 FITC (1:10) were from BD Biosciences. Anti-CD3 ECD (1:100) and anti-CD8β PE (1:50) were from Beckman Coulter. Anti-Vα7.2 FITC (1:10) and PE (1:20), anti-CD45 Alexa Fluor 700 (1:400), anti-CD45RO PECy7 (1:50), anti-CCR9 Alexa Fluor 647 (1:20), anti-Ki67 Brilliant Violet 421 (1:20), anti-CCR7 Brilliant Violet 421 (1:20) were from BioLegend. Anti-CD161 PerCP-Cy5.5 (1:20), anti-IL-22 PerCP-eFluor 710 (1:50) were from eBioscience. Anti-IL-18R PE (1:20), and anti-PLZF APC (1:10) were from R&D systems. Anti-CD4 Qdot 655 (1:400), anti-CD8α Qdot 605 (1:800), anti-CD45 Qdot 705 (1:100), live/dead aqua (1:100) and near infrared fixable (1:400) cell stain were from Invitrogen. Cell surface staining was performed using directly conjugated antibodies and fixed in Cytofix/Cytoperm (BD Biosciences). Intracellular staining was performed using the appropriate mAb’s in Perm/Wash (BD Biosciences). Samples were acquired on an LSRII flow cytometer (BD Biosciences) equipped with 405, 488, 532 and 647 nm lasers. Single-stained polystyrene beads (BD Biosciences) were used for compensation purposes. Software-based compensation was performed using the compensation platform in FlowJo software version 9.5 (Tree Star).

Leeansyah E., Loh L., Nixon D.F, & Sandberg J.K. (2014). Acquisition of innate-like microbial reactivity in mucosal tissues during human fetal MAIT-cell development. Nature Communications, 5, 3143.

+ Open protocol

+ Expand

Immunophenotyping of PBMC Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMCs were assessed for CD4 and CD8 cell phenotypic characterization, activation marker expression, and frequency of different populations of NK and B cells. PBMCs were stained with anti-CD3 PE/Dazzle 594 (Biolegend), anti-CD62L PE, and anti-CD31 PE/Cy7 for phenotypic characterization; anti-CD3 PE/Dazzle 594 (Biolegend), anti-CD8 APC/Cy7, anti-HLA-DR Percp5.5, anti-CD38 FITC, anti-CD25 APC, and anti-CD127 PE/Cy7 for activation marker analysis; and anti-CD3 PE/Cy7, anti-CD56 APC/Cy7, anti-CD16 FITC, anti-CD19 APC, and CD27 PE for NK and B cell phenotyping (all from BD Biosciences) in the second tube for 30 min at room temperature in the dark. CD4 cells in the first two tubes were identified as CD8 À CD3 + cells. The cells were washed and fixed by adding 3% formaldehyde and were acquired within 24 h to obtain 50 000 gated lymphocyte events by FACSAria Fusion (BD Biosciences). The data were analyzed using FACSDiva software.

Shete A., Dhayarkar S., Sangale S., Medhe U., Panchal N., Rahane G., Yelgate R., Dhamanage A, & Gangakhedkar R. (2019). Incomplete functional T-cell reconstitution in immunological non-responders at one year after initiation of antiretroviral therapy possibly predisposes them to infectious diseases. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 81.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!