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Unphosphorylatable β catenin

Manufactured by Addgene
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Unphosphorylatable β-catenin is a laboratory tool used for research purposes. It is a mutant form of the β-catenin protein that cannot be phosphorylated, which is a key regulatory mechanism for this protein. This specific variant allows researchers to study the functions and signaling pathways of β-catenin in a controlled setting.

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Lab products found in correlation

Phoenix eco cells, by American Type Culture Collection (2 mentions) Ha ubiquitin, by Addgene (2 mentions) Flag tagged dvl2, by Addgene (2 mentions) Retroviral plex expression system, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

β-catenin

2 protocols using unphosphorylatable β catenin

1

Epsin 1 Mutagenesis and Plasmid Construction

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Flag-tagged Dvl2, unphosphorylatable β-catenin, HA-ubiquitin and NICD-myc plasmids were obtained from Addgene. Retroviral pLEX expression system was from Thermoscientific. Epsin 1 and epsin 1 ΔUIM plasmids and lentiviral pLL3.7 RNA interference vectors encoding epsins 1 and 2 shRNA were described previously29 (link). All remaining truncation and single domain constructs were generated using standard subcloning procedures. Epsin 1 point mutations were made using QuikChange Site-Directed Mutagenesis Kit per manufacturer’s instruction. Epsin 1 point mutations were predicted using three-dimensional modeling by PEP-FOLD (http://bioserv.rpbs.univ-paris-diderot.fr/PEP-FOLD). Cloning and mutagenesis primers are available upon request. HEK 293T cells were transfected using Lipofectamine 2000 as instructed by the manufacturer. Lentiviral production was accomplished by transfecting Phoenix ECO cells (ATCC#CRL-3214) using Lipofectamine 2000. Viruses were harvested 48 hours post-transfection.
Chang B., Tessneer K.L., McManus J., Liu X., Hahn S., Pasula S., Wu H., Song H., Chen Y., Cai X., Dong Y., Brophy M.L., Rahman R., Ma J.X., Xia L, & Chen H. (2015). Epsin is required for Dishevelled stability and Wnt signaling activation in colon cancer development. Nature communications, 6, 6380.
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2

Epsin 1 Mutagenesis and Plasmid Construction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flag-tagged Dvl2, unphosphorylatable β-catenin, HA-ubiquitin and NICD-myc plasmids were obtained from Addgene. Retroviral pLEX expression system was from Thermoscientific. Epsin 1 and epsin 1 ΔUIM plasmids and lentiviral pLL3.7 RNA interference vectors encoding epsins 1 and 2 shRNA were described previously29 (link). All remaining truncation and single domain constructs were generated using standard subcloning procedures. Epsin 1 point mutations were made using QuikChange Site-Directed Mutagenesis Kit per manufacturer’s instruction. Epsin 1 point mutations were predicted using three-dimensional modeling by PEP-FOLD (http://bioserv.rpbs.univ-paris-diderot.fr/PEP-FOLD). Cloning and mutagenesis primers are available upon request. HEK 293T cells were transfected using Lipofectamine 2000 as instructed by the manufacturer. Lentiviral production was accomplished by transfecting Phoenix ECO cells (ATCC#CRL-3214) using Lipofectamine 2000. Viruses were harvested 48 hours post-transfection.
Chang B., Tessneer K.L., McManus J., Liu X., Hahn S., Pasula S., Wu H., Song H., Chen Y., Cai X., Dong Y., Brophy M.L., Rahman R., Ma J.X., Xia L, & Chen H. (2015). Epsin is required for Dishevelled stability and Wnt signaling activation in colon cancer development. Nature communications, 6, 6380.
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