Graph prism 5

The results are presented as the mean ± standard error mean (S.E.M.). Two-way ANOVA followed by Bonferroni's post hoc test was used to analyze paw volumes differences, with P values of < 0.05 considered significant. Statistical analyses were performed by using GraphPrism 5.0 (San Diego, USA) for Windows.

Statistical analyses were performed using SPSS version 20.0 and Graph prism 5.0. Results are expressed as mean ± SE of the mean for normally distributed data. Inter-group comparisons for body weights were performed using one-way ANOVA with correction for multiple comparisons by Tukey’s post hoc test; P < 0.05 was considered statistically different. Inter-group comparisons of ACF counts and tumor measurements were assessed with the 2 × 2 factorial designs; P < 0.05 was regarded as statistically significant for each main effect and interaction.

Cox proportional hazards were used to estimate lethality rate and hazard ratios (HR) between groups. Kruskal–Wallis with p < 0.05 as the significance level was chosen for evaluation of gene expression of cytokines/chemokines and the RABV N gene. Graph-Prism^® 5.0 and Instat^® softwares were used as analysis tools. Gene expression of cytokines/chemokines were first compared between treated and non-treated groups inoculated with the same variant, at the same period, 5 or 10 days p.i (ex: hv3 non-treated 5 days vs hv3 siRNA treated at 5 days); a second comparison was made between groups at the same condition, inoculated (with same viral variant) treated and non-treated but at different periods, 5 and 10 days (ex: hv2 siRNA treated at 5 days vs hv2 siRNA treated at 10 days).