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2 protocols using anti praptor 792

1

Protein Modification and Regulation Assays

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NButGT, Ac-5SGlcNAc were provided by D. Vocadlo (Simon Fraser University)(48 (link)). SRT1720, EX-527, Compound C purchased from Selleck-Chemicals (Houston, TX). MG132 purchased from Sigma-Aldrich (St. Louis, MO). pcDNA3.1-SIRT1-Flag was gift from E. Verdin (Addgene plasmid#: 13812). pLenti4-HA-OGT (provided by K. Vosseller, Drexel University). Antibodies used: anti-actin, anti-FOXM1 and anti-RL2 from Santa Cruz Biotechnology; pERK1/2, anti-OGT and anti-O-GlcNAc from Sigma-Aldrich; anti-acetylated p53(K382), anti-AMPK, anti-pAMPK(S172), anti-ERK1/2, anti-MMP2, anti-pRaptor(792), anti-Raptor, anti-SIRT1 and anti-Ubiquitin(K48) from Cell Signaling (Danvers, MA); anti-Cdh1 and anti-p53 from Neobiolabs; anti-MMP9 from Novus-Biologicals (Littleton, CO); integrin α5 and α6 are from BD-Pharmingen (San Jose, CA).
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2

Protein Modification and Regulation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
NButGT, Ac-5SGlcNAc were provided by D. Vocadlo (Simon Fraser University)(48 (link)). SRT1720, EX-527, Compound C purchased from Selleck-Chemicals (Houston, TX). MG132 purchased from Sigma-Aldrich (St. Louis, MO). pcDNA3.1-SIRT1-Flag was gift from E. Verdin (Addgene plasmid#: 13812). pLenti4-HA-OGT (provided by K. Vosseller, Drexel University). Antibodies used: anti-actin, anti-FOXM1 and anti-RL2 from Santa Cruz Biotechnology; pERK1/2, anti-OGT and anti-O-GlcNAc from Sigma-Aldrich; anti-acetylated p53(K382), anti-AMPK, anti-pAMPK(S172), anti-ERK1/2, anti-MMP2, anti-pRaptor(792), anti-Raptor, anti-SIRT1 and anti-Ubiquitin(K48) from Cell Signaling (Danvers, MA); anti-Cdh1 and anti-p53 from Neobiolabs; anti-MMP9 from Novus-Biologicals (Littleton, CO); integrin α5 and α6 are from BD-Pharmingen (San Jose, CA).
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