Iodoacetic acid

Manufactured by Thermo Fisher Scientific

Iodoacetic acid is a chemical compound used in various laboratory applications. It is a colorless crystalline solid with the molecular formula CH2ICOOH. Iodoacetic acid is commonly used as a reagent in biochemical and analytical procedures, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.

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Lab products found in correlation

Anti pan akt, by Cell Signaling Technology (2 mentions) Anti pser473 akt, by Cell Signaling Technology (2 mentions) Streptavidin hrp, by GE Healthcare (2 mentions) Zeba desalt spin column, by Thermo Fisher Scientific (2 mentions) Protease inhibitor mixture tablet, by Roche (2 mentions) Streptavidin chip, by GE Healthcare (2 mentions) Anti flag m2 bead, by Merck Group (2 mentions) Hrp conjugated secondary antibody, by Jackson ImmunoResearch (2 mentions) Anti ptp1b fg6, by Merck Group (2 mentions) Pt 66 agarose conjugated beads, by Merck Group (2 mentions) Anti ptp1b pser50 ab62320, by Abcam (2 mentions) Surfact amps nonidet p 40, by Thermo Fisher Scientific (2 mentions) Ez link biotin iodoacetyl peg2 biotin iap, by Thermo Fisher Scientific (2 mentions) Biacore sensor nta, by GE Healthcare (2 mentions) Anti flag m2 peroxidase, by Merck Group (2 mentions) Ha peroxidase, by Merck Group (2 mentions) Anti egfr, by Santa Cruz Biotechnology (2 mentions) Anti phospho egfr, by Cell Signaling Technology (2 mentions) Anti ha beads, by Merck Group (2 mentions) Gapdh, by Santa Cruz Biotechnology (2 mentions)

3 protocols using iodoacetic acid

Protein Interaction and Modification Analysis

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Anti-EGFR, GAPDH and 14-3-3ζ were from Santa Cruz Biotechnology. Anti-Pan-AKT, anti-AKT pSer⁴⁷³, anti phosphoEGFR antibodies were from Cell Signaling Technology. HA-peroxidase and anti-PTP1B (FG6) was from Millipore. PT-66-agarose-conjugated beads, anti-FLAG M2 beads, and anti-HA beads and anti-Flag M2 peroxidase were purchased from Sigma. Anti-PTP1B pSer⁵⁰ (Ab62320) were from Abcam. Streptavidin-HRP was from GE Healthcare. HRP-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories, Inc. Protease inhibitor mixture tablets were from Roche. Catalase and superoxide dismutase were from Calbiochem. Surfact-Amps Nonidet P-40, zeba desalt spin columns, EZ-Link biotin-iodoacetyl-PEG2 (biotin-IAP), and iodoacetic acid were from ThermoScientific. The pTyr loop-derived peptide (CKNRNRYRDVS) and phospho-Ser⁵⁰ pTyr loop-derived peptide (CKNRNRYRDVpS) were from GenScript USA Inc. BIACore sensor NTA and Streptavidin chips were from GE healthcare.

Londhe A.D., Bergeron A., Curley S.M., Zhang F., Rivera K.D., Kannan A., Coulis G., Rizvi S.H., Kim S.J., Pappin D.J., Tonks N.K., Linhardt R.J, & Boivin B. (2019). Regulation of PTP1B activation through disruption of redox-complex formation. Nature chemical biology, 16(2), 122-125.

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Lipid Raft Isolation by Sucrose Gradient

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Lipid rafts were separated as previously described^{35 (link)}. Briefly, cells were lysed on ice for 25 min in TNE buffer (5 mM iodoacetic acid (ThermoFisher Scientific), 150 mM NaCl, 10 mM Tris-HCl pH 7.4, 15 mM EDTA, Complete Protease Inhibitor) supplemented with 100 mM Na₂CO₃ and 0.5% Triton X-100. Lysates were sonicated and added to 60% Opti-Prep sucrose substitute (Sigma-Aldrich) to make a final concentration of 40% Opti-Prep. Lysates were placed in an ultracentrifuge tube and layered over with 30 and 5% solutions of Opti-Prep diluted with TNE buffer. Samples were centrifuged for 18 h at 200,000g at 4 °C. Eleven 1 mL fractions were taken sequentially from each sample. An equal volume of each fraction was used for immunoblot analysis.

Secinaro M.A., Fortner K.A., Dienz O., Logan A., Murphy M.P., Anathy V., Boyson J.E, & Budd R.C. (2018). Glycolysis promotes caspase-3 activation in lipid rafts in T cells. Cell Death & Disease, 9(2), 62.

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Protein Interaction and Modification Analysis

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