Ultrapure lipopolysaccharide lps

Nigericin, dimethyl sulfoxide, and ultrapure lipopolysaccharide (LPS) were purchased from Sigma (Munich, Germany). SolB, WeD, and colchicine were obtained from TargetMol (Boston, MA, United States). Anti-mouse-Collagen1 (1:1000), anti-mouse-Smad2/3 (1:1000), and anti-mouse-P-Smad3 (1:1000) were purchased from Cell Signaling Technology (Boston, MA, United States). Anti-mouse-P-IκBα (1:1000), anti-mouse-IκBα (1:1000), anti-mouse -P-IKK (1:1000), anti-mouse-IKK (1:1000), anti-human Smad3 (1:1000), anti-human P-Smad3 (1:1000), and anti-human α-SMA (1:1000) were purchased from Proteintech (Chicago, IL, United States). Anti-mouse-IL-1β, anti-mouse-NLRP3 (1:2000), and anti-mouse-ASC (1:1000) were purchased from Santa Cruz Biotechnology (Beijing, China). Anti-mouse -caspase-1 p20 (1:1000), anti-mouse-caspase-1 p45 (1:1000), anti-mouse-pro-IL-1β (1:1000), and anti-GAPDH (1:2000) were purchased from Proteintech (Chicago, IL, United States).

Dimethyl sulfoxide and ultrapure lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (Munich, Germany). Apigenin, esculetin, gomisin N, schisanhenol, schisandrin A, schisandrin B, anwulignan, schisantherin A, schisantherin B, specnuezhenide, schisandrin, luteolin, quinic acid, curcumenol, and acetaminophen (APAP) were obtained from MCE (New Jersey, NJ, United States). Anti-mouse-IL-1β, anti-mouse-NLRP3, and anti-mouse-ASC antibodies were purchased from Santa Cruz Biotechnology (Beijing, China). Anti-mouse-caspase-1 p45, anti-mouse-pro-IL-1β, and anti-GAPDH antibodies were purchased from Proteintech (Chicago, IL, United States). MitoSOX was purchased from Invitrogen (Carlsbad, CA, United States).

Human plasma-derived von Willebrand factor-Factor VIII free (VWF) (Haematology Technologies Inc.), Recombinant von Willebrand factor, VonVendi® (r-VWF) (Takeda), Ultra pure lipopolysaccharide (LPS) (Sigma), INFγ (Life Technologies, Gibco), IL-4 (Life Technologies, Gibco), IL-10 (Life Technologies, Gibco), IL-13 (Life Technologies, Gibco). Recombinant mouse M-CSF (rm M-CSF) (R&D System), Cell Tracker green CMFDA Dye (Thermofisher), Seahorse XF Cell Mito Stress Cell Kit (Agilent), Primary antibodies human anti-p38, P-p38, JNK, P-JNK, IKBα, P-IKBα, p65, P-p65, HIF-1α, and β-actin (Cell Signaling Technologies), Alexa Fluor 488 conjugate (Molecular Probes, Thermofisher Scientific).