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Bcl 2 15071

Manufactured by Cell Signaling Technology
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Bcl-2 (15071) is a primary antibody product offered by Cell Signaling Technology. It is an immunoglobulin that specifically binds to the Bcl-2 protein, which plays a role in regulating apoptosis, or programmed cell death.

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Lab products found in correlation

Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Rab27a, by Proteintech (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Nicotinamide, by Merck Group (1 mentions) Ammonium pyrrolidinedithiocarbamate pdtc, by Merck Group (1 mentions) Cleaved caspase 9 9509, by Cell Signaling Technology (1 mentions) Sirtuin antibody sampler kit, by Cell Signaling Technology (1 mentions) Nf κb p65 antibody sampler kit, by Cell Signaling Technology (1 mentions) Death receptor antibody sampler kit, by Cell Signaling Technology (1 mentions) Bax 5023, by Cell Signaling Technology (1 mentions) β actin sc 58673, by Santa Cruz Biotechnology (1 mentions) Cleaved caspase 3 9664, by Cell Signaling Technology (1 mentions) Cyt c 10993 1 ap, by Proteintech (1 mentions) Ab215191, by Abcam (1 mentions)

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Bcl-2 (2034 citations)

3 protocols using bcl 2 15071

1

Western Blot Analysis of Cellular Proteins

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Total proteins from cells were extracted in cell-lysis buffer (Pierce, Rockford, IL) and quantified using the Bradford method. About 50 μg protein was transferred to PVDF membranes after separated by SDS-PAGE. The membranes were incubated at 4°C overnight with Rab27A (1:800, Proteintech, USA), cyclin E (4129), cyclin D1 (2978), p-IκB (2859), IκB (4814), p-p65 (3033), p65 (8242), caspase 3 (9668), cleaved caspase 3 (9664), PARP (9532), cleaved PARP (5625), cytochrome c (11940), Bcl-2 (15071) (1:1000; Cell signaling, Boston, USA) and GAPDH (1:2000; Santa Cruz, USA). After incubation with HRP-coupled anti-mouse/rabbit IgG antibody (1:2000 dilution, Cell Signaling Technology, USA) at 37°C for 2 hours. Target proteins on PVDF membrane were visualized using ECL (Pierce, USA) and captured using a DNR BioImaging System (DNR, Israel).
Liu J., Gong X., Zhu X., Xue D., Liu Y, & Wang P. (2017). Rab27A overexpression promotes bladder cancer proliferation and chemoresistance through regulation of NF-κB signaling. Oncotarget, 8(43), 75272-75283.
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2

Apoptosis Signaling Pathway Analysis

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The following antibodies and reagents were used: Sirtuin Antibody Sampler Kit #9787T, NF‐κB p65 Antibody Sampler Kit #4767, Death Receptor Antibody Sampler Kit #8356, antibodies against cleaved PARP #5626, cleaved caspase‐3 #9664, cleaved caspase‐8 #8592, cleaved caspase‐9 #9509, Bak #6947, Bax #5023, Bcl‐2 #15071, and Bcl‐X #2764 (Cell Signaling Technology, Danvers, MA, USA); antibodies against HDAC3 #sc‐130319 and β‐actin #sc‐58673 (Santa Cruz Biotechnology, Dallas, TX, USA); nicotinamide (NAM); and ammonium pyrrolidine dithiocarbamate (PDTC) (Sigma‐Aldrich, St Louis, MO, USA).
Park J.Y., Han S., Ka H.I., Joo H.J., Soh S.J., Yoo K.H, & Yang Y. (2019). Silent mating‐type information regulation 2 homolog 1 overexpression is an important strategy for the survival of adapted suspension tumor cells. Cancer Science, 110(9), 2773-2782.
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3

Western Blot Analysis of GBM Cells Treated with AT7519

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Western blot analysis of GBM cells treated with AT7519 was performed as described previously [32 ]. Antibodies against p-RB (T821) (ab32015), RB (ab181616), and GSDME (ab215191) were purchased from Abcam. Antibodies against p-CDK1 (T161) (9114), CDK1 (9116), Cyclin B1 (12231), Caspase-3 (14220), Cleaved Caspase-3 (9661), Cleaved PARP (5625), Caspase-9 (9502), MCL-1 (94296), and BCL-2 (15071) were purchased from Cell Signaling Technology. Antibodies against GAPDH (60004-1-Ig), PARP1 (13371-1-AP), p-P53 (S15) (28961-1-AP), P53 (10442-1-AP), and Cyt-c (10993-1-AP) were purchased from Proteintech. Antibodies against p-CDK2 (T160) (AP0325), CDK2 (A0094), Cyclin E1 (A12000), and NOXA (A9801) were purchased from ABclonal.
Zhao W., Zhang L., Zhang Y., Jiang Z., Lu H., Xie Y., Han W., Zhao W., He J., Shi Z., Yang H., Chen J., Chen S., Li Z., Mao J., Zhou L., Gao X., Li W., Tan G., Zhang B, & Wang Z. (2023). The CDK inhibitor AT7519 inhibits human glioblastoma cell growth by inducing apoptosis, pyroptosis and cell cycle arrest. Cell Death & Disease, 14(1), 11.
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