The distribution of the cell cycle was detected using a cell cycle assay kit (Solarbio, Beijing, China). Briefly, HepG2 cells were cultured and incubated with selected samples according to the method mentioned above. After the cells treated for 48 h were collected, ice-cold PBS washing and 75% ethanol fixation (24 h, 4 °C) were carried out in turn. Then, following the washing of the fixed cells (1 × 106 cells/mL) with PBS again, 100 mL of RNase A was introduced and incubated with the cells for half an hour at 37 °C. Subsequently, the staining treatment was carried out with propidium iodide (PI) solution (50 μL). Half an hour later, a flow cytometer (LSR fortessa, BD Biosciences, Franklin Lakes, NJ, USA) was used for the detection of the cell cycle distribution.
Cell cycle assay kit
Manufactured by Solarbio
Sourced in China
The Cell Cycle Assay Kit is a laboratory tool designed to analyze the cell cycle distribution of a cell population. The kit provides reagents and protocols to quantify the percentage of cells in different phases of the cell cycle, such as G0/G1, S, and G2/M phases.
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Lab products found in correlation
2 protocols using cell cycle assay kit
1
Cell Cycle Distribution Analysis
2
Cell Cycle Analysis Using Flow Cytometry
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Cell cycle analysis was performed using cell cycle assay kit (Solarbio, Beijing, China) according to the kit instructions. Briefly, the cells were collected, washed and centrifuged at 1,000 rpm and then fixed with 75% cold ethanol at 4 ℃ overnight. The cells were washed and centrifuged at 1,000 rpm, treated with RNase A at 37 °C for 30
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