Integrin α5 (clone 5H10-27(MFR5)) and integrin αV (clone RMV-7) antibodies were purchased from BD Biosciences. Integrin β1 (clone HMβ1-1) was obtained from Biolegend and integrin β3 antibody (clone HMb3-1) from Millipore (MA, US). The secondary antibodies [biotin-SP-conjugated goat α-rat IgG, Rhodamine Red-X-AffiniPure goat α-Armenian hamster IgG(H + L)] and streptavidin-FITC were purchased from Jackson ImmunoResearch (West Grove, PA). For quantification of surface integrin expression, detached fibroblasts were incubated in suspension medium (DMEM containing 0.25% BSA) for 40 min at 37°C. Then, 2 × 105 cells were incubated with appropriate primary antibodies (diluted 1:300) in FACS buffer (5% heat-inactivated FCS, 1% sodium azide in PBS) for 1 hr at 4°C. After washing, secondary antibodies were applied for 1 h at 4°C, then washed again, before samples were analyzed by flow cytometry (LSRII, BD Biosciences).
Integrin α5 clone 5h10 27 mfr5
Manufactured by BD
Integrin α5 (clone 5H10-27(MFR5)) is a mouse monoclonal antibody that recognizes the human integrin α5 subunit. Integrins are cell surface receptors involved in cell adhesion and signaling.
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2 protocols using integrin α5 clone 5h10 27 mfr5
1
Quantifying Surface Integrin Expression
2
Quantifying Cell Surface Integrin Expression
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Integrin α 5 (clone 5H10-27(MFR5)) and integrin α V (clone RMV-7) antibodies were purchased from BD Biosciences. Integrin β 1 (clone HMβ1-1) was obtained from Biolegend and integrin β 3 antibody (clone HMb3-1) from Millipore (MA, US). Secondary antibodies (biotin-SP-conjugated goat α-rat IgG), streptavidin-FITC, and Rhodamine Red-X-AffiniPure Goat Anti-Armenian Hamster IgG (H+L) were purchased from Jackson ImmunoResearch (West Grove, PA). For quantification of surface integrin expression, suspended fibroblasts were incubated in suspension medium (DMEM containing 0.25% BSA) for 40 min at 37℃. Then, 2 × 10 5 cells were incubated with appropriate primary antibodies (diluted 1:300) in FACS buffer (5% heat-inactivated FCS, 1% sodium azide in PBS) for 1 hr at 4℃.
After washing, secondary antibodies were applied for 1 hr at 4℃, then washed again, samples were analyzed by flow cytometry (LSRII, BD Biosciences).
After washing, secondary antibodies were applied for 1 hr at 4℃, then washed again, samples were analyzed by flow cytometry (LSRII, BD Biosciences).
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