Tecnai g2 spirit biotwin 120 kv transmission electron microscope

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Tecnai G2 Spirit BioTWIN 120-kV transmission electron microscope is a high-performance imaging instrument designed for biological and material science applications. The microscope operates at a voltage of 120 kV and is capable of producing high-resolution images of samples on the nanometer scale.

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3 protocols using tecnai g2 spirit biotwin 120 kv transmission electron microscope

Negative Staining of Cyanophage Mic1

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The 4 μL suspension of freshly purified cyanophage Mic1 was layered onto a hydrophilized carbon-coated copper grid, and Mic1 particles were negatively stained with 2% uranyl acetate for 1 min. The particles were examined with a Tecnai G2 Spirit BioTWIN 120 kV transmission electron microscope (FEI Company).

Yang F., Jin H., Wang X.Q., Li Q., Zhang J.T., Cui N., Jiang Y.L., Chen Y., Wu Q.F., Zhou C.Z, & Li W.F. (2020). Genomic Analysis of Mic1 Reveals a Novel Freshwater Long-Tailed Cyanophage. Frontiers in Microbiology, 11, 484.

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Cyanophage Morphology Characterization

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To characterize the phage morphology, a drop of 3.5 μL sample containing purified cyanophages was layered onto a hydrophilized carbon-coated copper grid and incubated with 2% uranyl acetate for 1 min. The negatively stained particles were examined with a Tecnai G2 Spirit BioTWIN 120-kV transmission electron microscope (FEI Company, Hillsboro, USA).

Du K., Yang F., Zhang J.T., Yu R.C., Deng Z., Li W.F., Chen Y., Li Q, & Zhou C.Z. (2022). Comparative genomic analysis of five freshwater cyanophages and reference-guided metagenomic data mining. Microbiome, 10, 128.

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Phage Morphology Characterization

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The morphological characteristics of isolated phages were determined as previously described.⁴⁴ (link) High titer phage sample (10⁸ PFU/mL) was prepared after purification, placed on carbon-coated copper grids for 15 minutes for adsorption. After further drying, samples were stained with 2% uranyl acetate and examined in Tecnai G2 Spirit BioTWIN 120-kV transmission electron microscope (FEI Company, Hillsboro, USA) with magnification of 12000-16000 ×. Phage dimensions were measured using Image capture software ImageJ.

Hu M., Xing B., Yang M., Han R., Pan H., Guo H., Liu Z., Huang T., Du K., Jiang S., Zhang Q., Lu W., Huang X., Zhou C., Li J., Song W., Deng Z, & Xiao M. (2023). Characterization of a novel genus of jumbo phages and their application in wastewater treatment. iScience, 26(6), 106947.

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