The BD GasPak EZ anaerobic system (BD Biosciences, United States) was used to create an anaerobic condition. All the Plates containing the treated and untreated cells were set inside the anaerobic jar containing CO2 gas packs and an indicator to observe the proper maintenance of the anaerobic condition. The anaerobic system containing the plates was incubated inside a 37°C incubator, and the data was recorded after the same time periods as the aerobic conditions.
Gaspak ez anaerobic system
Manufactured by BD
Sourced in United States
The BD GasPak EZ anaerobic system is a laboratory equipment designed for the cultivation of anaerobic microorganisms. It provides a controlled anaerobic environment for the growth and cultivation of anaerobic bacteria and other anaerobic microorganisms.
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Lab products found in correlation
2 protocols using gaspak ez anaerobic system
1
Anaerobic Cell Culture Incubation
2
Astrocyte Hypoxia and Gintonin Treatment
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The astrocytes were exposed to a hypoxic environment using the BD GasPak™ EZ anaerobic system [15] (link). Following exposure to hypoxic conditions for 0, 0.5, 1, 2, or 12 h, the cells were washed. For re-oxygenation, the culture medium was replaced with fresh culture medium, and then the cells were treated with 0–0.3 μg/mL gintonin for 0, 0.5, 2, or 8 h under normal culture conditions, depending on the experimental protocol.
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