Cells were blocked with anti-human CD16/32 antibody (BD Biosciences) for 10 minutes (min) before adding fluorochrome-conjugated antibodies (Supplementary Table S1
Anti human cd16 32 antibody
Manufactured by BD
The Anti-human CD16/32 antibody is a laboratory tool used to detect the presence of CD16 and CD32 receptors on the surface of cells. It serves as a reagent to identify and characterize cell populations expressing these receptors, which are important for immune system function. The antibody provides a factual and unbiased means to analyze the expression of these cell surface markers.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using anti human cd16 32 antibody
1
Ex Vivo Complement Deposition Staining
2
Detecting Antilymphocyte Antibodies in COVID-19
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After blocking with anti-human CD16/32 antibody (BD Bioscience), two million HD PBMC were incubated for 30 min at room temperature (RT) in 50 µL of HD, COVID-19, or influenza patient plasma or serum. Plasma or serum pooled from at least 10 HD (HDp) was run on every assay, and its MFI was used to normalize the MFI of individual samples. Cells were washed three times with 1% BSA-PBS and stained with fluorescently labeled anti-human IgG and IgM antibodies (Supplementary Table S1
3
Cell Surface Phenotyping via Flow Cytometry
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Unless specified, viable cells were
first washed three times with FACS buffer (0.1 M PBS with 5g/L of
BSA, 1 g/L of sodium azide, and 2 mM of EDTA) via centrifugation (600g, 4 min)-redispersion method. The cell density was determined
and adjusted to 10 × 106 cells/mL and blocked with
human Fc blocker (antihuman CD16/32 antibody, 2 μg/million cells;
BD) at 4 °C for 20 min before being stained with desired antibody/antibodies
according to the manufacturer’s instructions. Stained cells
were washed three times with FACS buffer before analysis on a Biosafety
Level 2 (BSL2) Intellicyt iQue Screener PLUS flow cytometer in the
UNC Flow Cytometry Core Facility at the UNC School of Medicine. All
cells were analyzed within 2 h (at 4 °C) after staining and were
analyzed without fixing. All collected FACS data were analyzed through
a FlowJo V10.0.7 software pad.
first washed three times with FACS buffer (0.1 M PBS with 5g/L of
BSA, 1 g/L of sodium azide, and 2 mM of EDTA) via centrifugation (600g, 4 min)-redispersion method. The cell density was determined
and adjusted to 10 × 106 cells/mL and blocked with
human Fc blocker (antihuman CD16/32 antibody, 2 μg/million cells;
BD) at 4 °C for 20 min before being stained with desired antibody/antibodies
according to the manufacturer’s instructions. Stained cells
were washed three times with FACS buffer before analysis on a Biosafety
Level 2 (BSL2) Intellicyt iQue Screener PLUS flow cytometer in the
UNC Flow Cytometry Core Facility at the UNC School of Medicine. All
cells were analyzed within 2 h (at 4 °C) after staining and were
analyzed without fixing. All collected FACS data were analyzed through
a FlowJo V10.0.7 software pad.
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