Cells and tissues were lysed by a total protein extraction buffer (Beyotime Biotechnology, Shanghai, People's
Republic of China). Equal amounts of lysate samples were then separated by SDS-PAGE and immunoblotted with primary antibodies and corresponding HRP-labeled secondary antibodies. Immuno-reactive protein bands were visualized in dark room after incubated with a DAB substrate (Millipore, Darmstadt, Germany). The primary and secondary antibodies employed in this work including anti-FAK Rabbit mAb (1:1,000, #71433), anti-E-Cadherin Rabbit mAb (1:1000, #3195), anti-N-Cadherin Rabbit mAb (1:1,000, #13116), anti-Vimentin Rabbit mAb (1:1000, #5741), anti-β-Actin Rabbit mAb (1:1,000, #4970) and (HRP)-linked anti-rabbit IgG antibody (1:2000, #7074), which were all obtained from Cell Signaling Technology (Massachusetts, USA).
Republic of China). Equal amounts of lysate samples were then separated by SDS-PAGE and immunoblotted with primary antibodies and corresponding HRP-labeled secondary antibodies. Immuno-reactive protein bands were visualized in dark room after incubated with a DAB substrate (Millipore, Darmstadt, Germany). The primary and secondary antibodies employed in this work including anti-FAK Rabbit mAb (1:1,000, #71433), anti-E-Cadherin Rabbit mAb (1:1000, #3195), anti-N-Cadherin Rabbit mAb (1:1,000, #13116), anti-Vimentin Rabbit mAb (1:1000, #5741), anti-β-Actin Rabbit mAb (1:1,000, #4970) and (HRP)-linked anti-rabbit IgG antibody (1:2000, #7074), which were all obtained from Cell Signaling Technology (Massachusetts, USA).