To build on knowledge regarding the red cell storage lesion of canine CSWB, serial percent hemolysis was calculated for both NGD and GD CSWB at d21, d28, d35, and d42. Percent hemolysis was calculated as previously described: % hemolysis = [supernatant hemoglobin (g/L) × (100 – packed cell volume)] / total hemoglobin (g/L) (43 (link)). Total hemoglobin concentration (g/L) was measured from a citrated whole blood aliquot (Hemocue® Hb 201+ System, Hemocue®, Ängelholm, Sweden) at the same time as ROTEM analysis, and supernatant hemoglobin was measured in citrated plasma (Hemocue® Plasma/Low Hb system, Hemocue®, Ängelholm, Sweden) after centrifugation (4,000 g for 10 min at 4°C; Thermo ScientificTM CryofugeTM 5500i centrifuge, Thermo Fisher ScientificTM, MA, USA). Concurrently, a packed cell volume was determined on the citrated whole blood aliquots.
Plasma low hb system
Manufactured by HemoCue
Sourced in Sweden
The Hemocue® Plasma/Low Hb system is a lab equipment designed to measure hemoglobin levels in plasma and low-hemoglobin samples. It provides accurate and reliable results for clinical use.
Automatically generated - may contain errors
Lab products found in correlation
Hb 201 system, by HemoCue (1 mentions)
Abx pentra 400, by Horiba (1 mentions)
Abx pentra xl80, by Horiba (1 mentions)
Modular e170 analyzer, by Roche (1 mentions)
Coulter lh 750 hematology analyzer, by Beckman Coulter (1 mentions)
Architect i2000 analyzer, by Abbott (1 mentions)
Immage 800 analyzer, by Beckman Coulter (1 mentions)
3 protocols using plasma low hb system
1
Canine Red Cell Storage Lesion Kinetics
2
Standardized Blood Biochemical Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sample supernatant was used to test potassium, phosphate, sodium, glucose and LDH using the ABX Pentra 400 chemistry analyser (Horiba ABX SAS, Montpellier, Cedex, France). The pH was measured using a desktop pH meter (Amtech Laboratory Services, Cape Town, South Africa). The haematological levels for the full blood count, haemoglobin, haematocrit, mean cell volume, mean corpuscular haemoglobin and the mean corpuscular haemoglobin concentrate were measured using the ABX Pentra XL 80 (Horiba ABX SAS, Montpellier, Cedex, France). The Hemocue® Plasma/Low Hb System (SE-262-71, Hemocue AB, Ängelholm, Sweden) was used to measure the plasma haemoglobin levels. The formula for calculating the percentage of plasma haemolysis is detailed below.
Equation 1 : Calculation for percentage plasma haemolysis (Adapted from Leitner, 2001)22 (link)
Testing for the haematological factors, biochemical indices and routine donor testing commenced within the accepted 72 h post-collection and is known as Day 1.
Testing for the haematological factors, biochemical indices and routine donor testing commenced within the accepted 72 h post-collection and is known as Day 1.
3
Hematological Changes in Endurance Athletes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Using sterile techniques, blood (20 mL) was drawn from an antecubital vein from each subject 1 week before and immediately after the race. All specimens were refrigerated and transported to the laboratory within 4 h of sampling. Red blood cell (RBC) count, hemoglobin, hematocrit, mean cell volume (MCV), mean cell hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width, coefficient of variation (RDW-CV) and reticulocyte were performed on a Coulter LH 750 Hematology Analyzer (Beckman Coulter, Miami, FL, USA), which was based on impedance detection for counting and sizing the blood cells. The evaluated plasma haptoglobin was assayed by the rate nephelometry on an Immage 800 Analyzer (Beckman Coulter, Fullerton, CA, USA). Free plasma hemoglobin was tested with a HemoCue Plasma/Low Hb System (HemoCue, Lake Forest, CA, USA) utilizing the azide-methemoglobin method. Ferritin levels were determined by an Architect I-2000 Analyzer (Abbott Diagnostics, Abbott Park, IL, USA), which used a chemiluminescent microparticle immunoassay. Iron and unsaturated iron-binding capacity (UIBC) were measured on a Modular E170 Analyzer (Roche Diagnostics, Mannheim, Germany) using an electrochemiluminescence immunoassay.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!