Sheta2

SHetA2 was synthesized by Cayman Chemical company, Inc. under a contract from the
Rapid Access to Preventive Intervention Development (RAPID) National Cancer Institute
(NCI) program. Cocoa butter was purchased from Nature’s Oils (Streetsboro, OH).
Polyethylene glycols (PEG) (MW 400, 3350), acetonitrile (HPLC grade
≥99.5%), sodium chloride, lactic acid, acetic acid, potassium hydroxide
(KOH), bovine serum albumin (BSA), glucose were purchased from Sigma Aldrich (St Louis,
MO). Glycerol USP and mineral oil USP were purchased from VWR International (Radnor, PA).
Kolliphor was obtained from BASF (Germany). Captiva^® filtration plates
were purchased from Agilent Technologies Inc. Anti-cyclin D1 antibody was purchased from
Cell Signaling Technology (Boston, MA), and anti-β-actin peroxidase/FITC
conjugated secondary antibodies were purchased from Santa Cruz (Santa Cruz, CA) for
Western Blot experiments. Mammalian Protein Extraction Reagent (m-PER), Pierce BCA protein
assay kits were purchased from Thermo Fisher Scientific (Grand Island, NY). Protease
inhibitor cocktail and phosphatase inhibitor cocktail were purchased from Roche (New York,
NY). Polyvinylidene difluoride (PVDF) membrane and enhanced chemiluminescence (ECL)
reagent were purchased from Bio-Rad (Hercules, CA).

SHetA2 was synthesized by Cayman Chemical company, Inc. (Ann Arbor, MI) under a contract from the Rapid Access to Preventive Intervention Development (RAPID) National Cancer Institute (NCI) program. Cocoa butter was purchased from Nature’s Oils (Streetsboro, OH). Kolliphor HS15 was obtained from BASF (Germany). Sterile saline and isoflurane were obtained from Henry Schein Animal Health Inc. Acetonitrile (HPLC grade ≥ 99.5%), methanol (HPLC grade ≥ 99.5%), phosphoric acid, hydrochloric acid, crystal violet stain and sodium acetate trihydrate were purchased from Sigma Aldrich (St Louis, MO). Captiva^® filtration equipment was purchased from Agilent Technologies Inc. for extraction of drug from tissues. Mouse cyclin D1 enzyme linked immunosorbent assay kit (ELISA) was purchased from Cedarlane lab (NC). T-PER (tissue protein extraction reagent) was purchased from Thermo-Fisher Scientific (Waltham, MA). Protease inhibitor cocktail tablets were purchased from Sigma Aldrich (St Louis, MO).

SHetA2 was synthesized by Cayman Chemical company, Inc. under a contract from the Rapid Access to Preventive Intervention Development (RAPID) National Cancer Institute (NCI) program. Cocoa butter was purchased from Nature’s Oils (Streetsboro, OH). Kolliphor was obtained from BASF (Germany). Sterile saline and isoflurane were obtained from Henry Schein Animal Health Inc. Acetonitrile (HPLC grade ≥ 99.5 %), methanol (HPLC grade ≥ 99.5 %), phosphoric acid, hydrochloric acid, crystal violet stain and sodium acetate trihydrate were purchased from Sigma Aldrich (St Louis, MO). Captiva^® filtration equipment was purchased from Agilent Technologies Inc. for extraction of drug from tissues. Mouse cyclin D1 enzyme linked immunosorbent assay kit (ELISA) was purchased from Cedarlane lab (NC). T-PER (tissue protein extraction reagent) was purchased from Thermo-Fisher Scientific (Waltham, MA). Protease inhibitor cocktail tablets were purchased from Sigma Aldrich (St Louis, MO).