Beclin 1

Manufactured by Beyotime

Sourced in China

Beclin 1 is a key regulator of autophagy, a cellular process involved in the degradation and recycling of damaged or unwanted cellular components. As a core component of the autophagy machinery, Beclin 1 plays a critical role in the initiation and regulation of autophagosome formation.

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Lab products found in correlation

Sqstm1 p62, by Beyotime (2 mentions) 2 7 dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions) Alexa fluor 488 conjugated goat anti mouse igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 conjugated goat anti rabbit igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions) Catalase, by Beyotime (1 mentions) Aicar, by MedChemExpress (1 mentions) N acetyl cysteine (nac), by Beyotime (1 mentions) Flag tag (flag), by Proteintech (1 mentions) β actin, by Proteintech (1 mentions) Tom20, by Beyotime (1 mentions) Cytochrome c, by Proteintech (1 mentions) Sars cov 2 np, by Sino Biological (1 mentions) Lc3a b, by Cell Signaling Technology (1 mentions) Tim23, by Proteintech (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Amersham imager 600, by GE Healthcare (1 mentions) Bcl 2, by Abmart (1 mentions) Gpx 4, by Bioss Antibodies (1 mentions) Amersham imager, by Cytiva (1 mentions) Cleaved caspase 3, by Abcam (1 mentions)

4 protocols using beclin 1

Mitochondrial Dynamics and Oxidative Stress

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FAC, calcein acetoxymethyl ester (CA-AM), deferiprone (L1) and 2',7'-dichlorofluorescin diacetate (DCFH-DA) were purchased from Sigma-Aldrich (USA). DFO was from Novartis Pharma (Switzerland). NAC, Catalase, Beclin-1, ATG5 and ATG7 were from Beyotime Biotechnology (Shanghai, China). AICAR was from Med Chem Express (USA). AMPK, p-AMPK (Thr172), p-Drp1 (Ser616), p-Drp1 (Ser637), p-ACC (ser79), LC3, GAPDH and VDAC1 were from Cell Signalling Biotechnology (Danvers, MA, USA). MFF was from Abcam (Cambridge, MA, USA). p-MFF (ser155) was generated by YenZym Antibodies (South San Francisco, CA, USA). TOM20 and Drp1 were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) secondary antibody, Alexa Fluor 488-conjugated goat anti-mouse IgG (H + L) secondary antibody and MitSOX^TM Red Mitochondrial Superoxide Indicator were from Thermo Scientific (Rockford, IL, USA).

Zheng Q., Zhao Y., Guo J., Zhao S., Fei C., Xiao C., Wu D., Wu L., Li X, & Chang C. (2018). Iron overload promotes mitochondrial fragmentation in mesenchymal stromal cells from myelodysplastic syndrome patients through activation of the AMPK/MFF/Drp1 pathway. Cell Death & Disease, 9(5), 515.

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SARS-CoV-2 Protein Localization and Interaction

Cited 1 time

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The Nsp1–10 and Nsp12–16 fragments were obtained from the SARS-CoV-2 (MN908947.3) cDNA provided by Prof. Ke Peng (Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China) (Li et al., 2020 (link)). The Nsp1–10, Nsp12–16, and N- and C-terminal domains of Nsp8 fragments were cloned into pCMV-Flag vector. And the full-lenth, N- and C-terminal domains of Nsp8 were cloned into pCMV-GFP vector to generate pCMV-GFP-Nsp8, pCMV-GFP-Nsp8-N and pCMV-GFP-Nsp8-C plasmids. Flag-Nsp8 was cloned into pCDH-puro-EGFP to generate the pCDH-Nsp8-puro-EGFP plasmid. Supplementary Table S1 lists the primers used in plasmid construction. Mito-DsRed2-EGFP (Cat#P4954) and mCherry-EGFP-LC3 (Cat#P0446) plasmids were purchased from Miaoling Plasmid Sharing Platform (Wuhan, China). EGFP-LC3 was cloned by mutation of mCherry-EGFP-LC3. The following primary antibodies were used: TOM20 (Beyotime, AF1717); SQSTM1/p62 (Beyotime, AF5312); Beclin 1 (Beyotime, AF5123); ATG5 (Beyotime, AF2269); LC3A/B (Cell Signaling Technology, 12741S); anti-Mouse IgG 647 (Invitrogen, A31571); anti-Mouse IgG594 (Invitrogen, A21203); Flag (Proteintech, 20543-1-AP); β-Actin (Proteintech, 66009-1-Ig); Cytochrome c (Proteintech, 10993-1-AP); COXII (Proteintech, 55070-1-AP); TIM23 (Proteintech, 11123-1-AP); SARS-CoV-2-NP (Sino Biological, 40143-MM05).

Zong S., Wu Y., Li W., You Q., Peng Q., Wang C., Wan P., Bai T., Ma Y., Sun B, & Qiao J. (2023). SARS-CoV-2 Nsp8 induces mitophagy by damaging mitochondria. Virologica Sinica, 38(4), 520-530.

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Western Blot Analysis of Apoptosis-Related Proteins

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Total proteins were loaded by 10% SDS-PAGE, transferred to polyvinylidene difluoride membranes, and blocked with 5% skim milk. Membranes were incubated with the following primary antibodies at 4°C overnight: GPX-4 (#bs-3884R; Bioss, China), B-cell lymphoma 2 (Bcl-2; # T40056; Abmart, China), Bcl-2-associated X protein (Bax; #T40051; Abmart, China), cleaved caspase-3 (#9644T; Cell signaling technology, USA), DNA damage-inducible transcript 3 protein/enhancer-binding protein homologous protein (DDIT3/CHOP; #AF6684; Beyotime Biotechnology, China), Beclin 1 (#AF5123; Beyotime Biotechnology, China), SQSTM1/p62 (#AF5312; Beyotime Biotechnology, China), ATG5 (#T55766; Abmart, China), and LC3B (#T55992; Abmart, China). A digital gel image analysis system Amersham Imager 600 (GE Healthcare Bio-Sciences, USA) was used to measure and analyze the density of the bands.

Wang L., Liu S., Gao C., Chen H., Li J., Lu J., Yuan Y., Zheng X., He H., Zhang X., Zhang R., Zhang Y., Wu Y., Lin W, & Zheng H. (2022). Arsenic trioxide-induced cardiotoxicity triggers ferroptosis in cardiomyoblast cells. Human & experimental toxicology, 41.

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Western Blot Analysis of Autophagy and Apoptosis Markers

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Total protein from CIHP-1 cells was extracted by RIPA (Beyotime), and denatured at 98 °C for 7 min before separation, and then transferred to polyvinylidene difluoride (PVDF, Beyotime) membranes. Membranes were sealed with 5% milk for 2 h before incubation with primary antibodies against B-cell lymphoma-2 (BCL-2, 1:1000, Abcam, Cambridge, MA, USA), Cleaved-caspase-3 (1:500, Abcam), Light chain 3-II (LC3-II, 1:3000, Abcam), LC3-I (1:1000, Abcam), Beclin 1 (1:2000, Beyotime), PPARγ (1:500, Abcam) or GAPDH (1:2000, Beyotime) overnight at 4 °C. HRP-conjugated secondary antibody (1:4000, Abcam) was employed to incubate the membranes for another 1 h. And the proteins were visualized by using BeyoECL Moon (Beyotime).

Li F., Dai B, & Ni X. (2020). Long non-coding RNA cancer susceptibility candidate 2 (CASC2) alleviates the high glucose-induced injury of CIHP-1 cells via regulating miR-9-5p/PPARγ axis in diabetes nephropathy. Diabetology & Metabolic Syndrome, 12, 68.

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