S8628

C57BL/6 male mice (7–8 weeks) weighing 18–22 g were obtained from the animal lab center of Southern Medical University (Guangzhou, China). The animal experiments were complied with the ARRIVE guidelines and conducted in strict accordance with the recommendations in the Guidelines for the Care and Use of Laboratory Animals (National Institutes of Health, Bethesda, MD, USA). The research programme was approved by the Ethics Committee of Animal Experiments of Southern Medical University. The sepsis mice was established using CLP method as described before [48 (link)]. All mice were anesthetized with pentobarbital (50 mg/kg) before the procedure. The chemical reagents with doses of 30 mg/kg melatonin (MCE, HY-B0075), 5 mg/kg 3-TYP (Selleck, S8628) and 50 mg/kg chloroquine (Selleck, S6999) were administered by intraperitoneal injection for consecutive 3 days before CLP. Mice were sacrificed 24 h after CLP for samples of blood and kidney tissue.
For survival study, mice were returned to their cages after awakening from anesthesia. 10 mice were included in each group and recorded every 2 h over a 5-day period. Apnea for more than 1 min was considered to indicate death. Mice that survived for more than 5 days were sacrificed by cervical dislocation.