All biomarkers were analysed through commercially available kits. PTX3 was quantified in plasma samples through an enzyme-linked immunosorbent assay (ELISA) kit (Human Pentraxin 3/TSG-14 Quantikine ELISA Kit, R&D Systems, Minnesota, USA). Three classical inflammatory biomarkers were evaluated in serum samples: high-sensitivity (hs) CRP by immunoturbidimetry (Cardiac C-Reactive Protein (Latex) High Sensitive assay, Roche Diagnostics, Basel, Switzerland), IL-6, and TNF-α (Human IL-6 Quantikine HS and Human TNF-alpha Quantikine HS, R&D Systems). Lipid profile, including total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C), was performed using laboratorial routine procedures (Cobas Integra 400 Plus autoanalyser; Roche Diagnostics, Basel, Switzerland); plasma oxidized LDL (oxLDL) and serum adiponectin, and leptin levels were determined through ELISA kits (Oxidized LDL ELISA Kit, Mercodia AB, Uppsala, Sweden; Human Total Adiponectin/Acrp30 Quantikine ELISA Kit and Human Leptin Quantikine ELISA Kit, R&D Systems). Levels of plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) and serum tissue inhibitor of metalloproteinase-1 (TIMP-1) (Human proBNP and Human TIMP1 ELISA kits, Abcam, Cambridge, UK) were assessed as cardiac and renal fibrosis markers, respectively.
Human tnf alpha quantikine hs
Manufactured by R&D Systems
Sourced in Switzerland, United States
The Human TNF-alpha Quantikine HS is a quantitative sandwich enzyme immunoassay designed for the measurement of human tumor necrosis factor alpha (TNF-alpha) concentrations in cell culture supernates, serum, and plasma. It has a high sensitivity with a minimum detectable dose typically less than 0.106 pg/mL.
Automatically generated - may contain errors
Lab products found in correlation
Human il 6 quantikine hs, by R&D Systems (2 mentions)
Human pentraxin 3 tsg 14 quantikine elisa kit, by R&D Systems (1 mentions)
Cardiac c reactive protein latex high sensitive assay, by Roche (1 mentions)
Oxidized ldl elisa kit, by Mercodia (1 mentions)
Human leptin quantikine elisa kit, by R&D Systems (1 mentions)
Human total adiponectin acrp30 quantikine elisa kit, by R&D Systems (1 mentions)
2 protocols using human tnf alpha quantikine hs
1
Comprehensive Biomarker Assessment Protocol
2
Cytokine Biomarker Sampling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected at 8 am, centrifuged, and stored at −80°C until thawed for assay. Participants were instructed to avoid exercise and alcohol 24 hours prior to blood withdrawal. Also, participants were instructed to avoid rushing and taking public transportation to the laboratory to avoid physical overexertion before blood sampling. Serum concentrations of IL-6 and TNF-alpha were determined using highly sensitive immunoassays according to the manufacturer’s instructions (Human IL-6 Quantikine HS and Human TNF-alpha Quantikine HS; R & D Systems, Minneapolis, MN, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!