Anti opn antibody

Manufactured by R&D Systems

Sourced in United States

The Anti-OPN antibody is a laboratory reagent used for the detection and quantification of osteopontin (OPN) in various samples. OPN is a protein involved in various biological processes. The antibody can be used in techniques such as Western blotting, ELISA, and immunohistochemistry to measure OPN levels.

Automatically generated - may contain errors

Lab products found in correlation

Anti phospho chk2 thr68, by Cell Signaling Technology (1 mentions) Anti mouse alexa 488, by Thermo Fisher Scientific (1 mentions) Isopropyl β d 1 thiogalactopyranoside iptg, by Merck Group (1 mentions) Anti 53bp1, by Novus Biologicals (1 mentions) Bond max automated immunostainer, by Leica (1 mentions) Bond polymer refine detection kit, by Leica (1 mentions) Axiocam camera, by Zeiss (1 mentions) Streptavidin qdot 655, by Thermo Fisher Scientific (1 mentions) Mac 3 antibody, by BD (1 mentions) Axioskope microscope, by Zeiss (1 mentions) Mmp 7, by Merck Group (1 mentions)

4 protocols using anti opn antibody

Antibody Immunoblotting for DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-OPN antibody was purchased from R&D Systems. Anti-Phospho H2AX (ser139, clone JBW301) and anti-ATM antibodies were purchased from Millipore. Anti-53BP1 was purchased from Novus Biologicals. Anti-Phospho ATM (ser1981), anti-Phospho 53BP1 (ser1778), anti-Chk2 and anti-Phospho Chk2 (thr68) antibodies were purchased from Cell Signalling. Anti-beta actin (clone AC-15) antibody was purchased from Sigma Aldrich. Anti-mouse Alexa 488 was purchased from Life Technologies. Secondary antibody rabbit anti-mouse HRP was purchased from Dako; secondary antibody donkey anti-goat HRP was purchased from Santa Cruz and secondary antibody goat anti-rabbit HRP was purchased from Invitrogen. IPTG (isopropyl β-D-1-thiogalactopyranoside) was purchased from Sigma Aldrich. Recombinant human osteopontin (full length) was a kind gift of Dr. Larry W. Fisher (National Institute of Dental and Craniofacial Research, Bethesda, MD, USA).

Henry A., Nokin M.J., Leroi N., Lallemand F., Lambert J., Goffart N., Roncarati P., Bianchi E., Peixoto P., Blomme A., Turtoi A., Peulen O., Habraken Y., Scholtes F., Martinive P., Delvenne P., Rogister B., Castronovo V, & Bellahcène A. (2016). New role of osteopontin in DNA repair and impact on human glioblastoma radiosensitivity. Oncotarget, 7(39), 63708-63721.

+ Open protocol

+ Expand

Tissue Microarray Analysis of HCC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue-microarray (TMA) slides containing 58 HCC samples were purchased from SuperBioChips Laboratories (Seoul, Korea, www.tissue-array.com). Immunohistochemistry (IHC) for OPN and EGFR on serially sectioned TMA slides was carried out using the BOND-MAX automated immunostainer (Leica Microsystems, Bannockburn, IL, USA) with the Bond Polymer Refine detection kit (Leica). Anti-OPN antibody (1:50; R&D Systems) or anti-EGFR antibody (1:100; Ventana Medical Systems, Oro Valley, AZ, USA) were employed as the primary antibody. Each stain was assessed according to the intensity (negative, 0; weak, 1; moderate, 2; intense, 3) and area (none, 0; focal, 1; multifocal, 2; diffuse, 3) of positive cells. The overall grade of each stain was obtained by multiplying the area score by the intensity score (negative, 0; weak, 1 or 2; moderate, 3 or 4; intense, 6 or 9).

Lee S.H., Park J.W., Woo S.H., Go D.M., Kwon H.J., Jang J.J, & Kim D.Y. (2016). Suppression of osteopontin inhibits chemically induced hepatic carcinogenesis by induction of apoptosis in mice. Oncotarget, 7(52), 87219-87231.

+ Open protocol

+ Expand

Aortic Histopathology and Macrophage Infiltration

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were euthanized, tissues pressure perfused with saline and fixed with 10% buffered formalin. Whole aortas were excised and paraffin embedded. Sections (5 lm) were stained with hemotoxylin & Eosin (H&E) for morphology and immunostained with Mac3 antibody (BD Pharmingen) to determine macrophage infiltration. Medial thickness was analyzed using NIH Image J. For the OPN antibody staining, antigen retrieval was performed using protease K (10 μg/mL) for 30 min, before incubation with anti-OPN antibody (R&D), followed by incubation with -anti-goat secondary antibody (Vector Labs) and incubation with Streptavidin QDot 655 (Invitrogen). Images were acquired with a Zeiss Axioskope microscope equipped with an AxioCam camera.

Caesar C., Lyle A.N., Joseph G., Weiss D., Alameddine F.M., Lassègue B., Griendling K.K, & Taylor W.R. (2016). Cyclic Strain and Hypertension Increase Osteopontin Expression in the Aorta. Cellular and Molecular Bioengineering, 10(2), 144-152.

+ Open protocol

+ Expand

Generation and Validation of MMP-Cleaved OPN

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human recombinant full-length OPN (flOPN) (Peprotech, Rocky Hill, NJ) was used for cell experiments. To obtain MMP-cleaved OPN (MMP-cOPN), 1 mg of OPN per ml was incubated with 0.5 ng per ml of MMP-7 (Millipore, Billerica, MA) for 4 h at 37 C in assay buffer (50 mM Tris, 150 mM NaCl, CaCl 2 2.5 mM, Brij-35 0.05%, pH adjusted to 7.6 with HCl) as earlier described (10) , of note the related "vehicle" controls included the same amount of MMP-7. Cleavage was evaluated qualitatively by immunoblotting according to Ref. 10) using a polyclonal anti-OPN antibody (RD-Systems, Minneapolis, MN).

Leitner L., Schuch K., Jürets A., Itariu B.K., Keck M., Grablowitz V., Aszmann O.C., Prager G., Staffler G., Zeyda M, & Stulnig T.M. (2015). Immunological blockade of adipocyte inflammation caused by increased matrix metalloproteinase-cleaved osteopontin in obesity. Obesity (Silver Spring, Md.), 23(4).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!