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Jwh 018

Manufactured by Cayman Chemical
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Sourced in United States

JWH-018 is an organic compound that can be used for research purposes in analytical chemistry laboratories. It serves as a reference standard for analytical procedures and may be used in the development and validation of analytical methods.

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Lab products found in correlation

Ur144, by Cayman Chemical (2 mentions) Xlr 11, by Cayman Chemical (2 mentions) Jwh 133, by Cayman Chemical (2 mentions) Am1220, by Cayman Chemical (2 mentions) Am251, by Cayman Chemical (1 mentions) Am 2201, by Cayman Chemical (1 mentions) Jwh 122, by Cayman Chemical (1 mentions) Cp55 940, by Cayman Chemical (1 mentions) Cannabidiol, by Cayman Chemical (1 mentions) Sr144528, by Cayman Chemical (1 mentions) Am404, by Cayman Chemical (1 mentions) Vdm11, by Cayman Chemical (1 mentions) Hu 210, by Cayman Chemical (1 mentions) Bms309403, by Cayman Chemical (1 mentions) Ab fubinaca, by Cayman Chemical (1 mentions) Win55 212 2, by Cayman Chemical (1 mentions) Capsaicin, by Merck Group (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) C18 cartridge, by Phenomenex (1 mentions)

6 protocols using jwh 018

1

Cannabinoid Dose Conversion for Humans

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Δ9-THC was provided by Dr. S. Morimoto (Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan) and stored in ethanol at −80 °C. JWH-018, metabolites of JWH-018, deuterium-labelled JWH-018 (JWH-018-d9) and AM-251 were purchased from Cayman Chemical (Ann Arbor, MI, USA) and stored at −20 °C. The doses of cannabinoids presently used here were selected based on the CB1R affinity of the ligands and the electrographic representation of mild seizure events. In fact, we intentionally selected relatively high doses that we knew to have an effect on EEG power spectra39 (link). The conversion of an animal to human dose cannot be done directly, as in the few available studies on human volunteers most utilize the inhalation route, while we administered drugs intraperitoneally. However, after applying the human estimation dose conversion40 (link), 2.5 mg/kg of JWH-018 in mouse corresponds to 0.2 mg/kg, or roughly 12 mg per person. Similarly, 10 mg/kg of ∆9-THC in mice converts to 0.8 mg/kg in humans or about 49 mg per individual.
Malyshevskaya O., Aritake K., Kaushik M.K., Uchiyama N., Cherasse Y., Kikura-Hanajiri R, & Urade Y. (2017). Natural (∆9-THC) and synthetic (JWH-018) cannabinoids induce seizures by acting through the cannabinoid CB1 receptor. Scientific Reports, 7, 10516.
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2

Characterization of Synthetic Cannabinoids

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All solvents were high-performance liquid chromatography (HPLC) grade, purchased from Fisher Scientific (Fair Lawn, NJ), EMD Chemical (Gibbstown, NJ), and Sigma-Aldrich (St. Louis, MO). JWH-018, AM2201, JWH-122, UR144, XLR11, and 5F-AKB48 reference standards were obtained from Cayman Chemical (Ann Arbor, MI). JWH-019 reference standard was synthesized in the laboratory of Dr. John W. Huffman and provided by Dr. Jenny L. Wiley. Herbal products were obtained from the Richland County Sheriff’s Department (Columbia, SC). These products were seized between December 2011 and August 2013 and were scheduled for destruction. Custody was turned over to RTI International, and products were stored in accordance with established protocols.
Moore K.N., Garvi D., Thomas B.F, & Grabenauer M. (2017). Identification of Eight Synthetic Cannabinoids, Including 5F-AKB48 in Seized Herbal Products Using DART-TOF-MS and LC-QTOF-MS as Nontargeted Screening Methods. Journal of forensic sciences, 62(5), 1151-1158.
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3

Comprehensive Cannabinoid Pharmacology Protocol

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The native phytocannabinoids Δ9-tetrahydrocannabinol (Δ9-THC, also called dronabinol, psychoactive high affinity CB1 and CB2 agonist) and cannabidiol (non-psychoactive, very low affinity CB1 and CB2 indirect antagonist thought to attenuate the action of Δ9-THC) were obtained from Cayman Chemical (Ann Arbor, MI). Synthetic cannabinoids obtained from Cayman Chemical (Ann Arbor, MI) were as follows: dronabinol (also called Δ9-tetrahydrocannabinol or Δ9-THC; high affinity CB1 and CB2 agonist), HU-210 (potent CB1 and CB2 receptor agonist analogue of Δ9-THC), JWH 018 (high affinity CB1 and CB2 ligand, mildly selective for CB2); Rimonabant high affinity selective CB1 receptor inverse agonist), JWH-133 (high affinity, 200-fold selective CB2 agonist), SR-144528 (high affinity CB2 inverse agonist), CP55,940 (high affinity non-selective CBR agonist, more potent than Δ9-THC). Synthetic cannabinoids that inhibit AEA uptake without affecting AEA hydrolysis (AM404), inhibit AEA uptake and weakly inhibit AEA hydrolysis (OMD1, OMD2), or inhibit AEA hydrolysis (VDM11) were purchased from Cayman Chemical (Ann Arbor, MI). BMS309403, known to bind/inhibit other FABPs (FABP3,4,5,7) was obtained from Cayman Chemical (Ann Arbor, MI). SCP2 inhibitors (SCPI1, SCPI3, and SCPI4) were from ChemBridge Corporation (San Diego, CA).
Huang H., McIntosh A.L., Martin G.G., Landrock D., Chung S., Landrock K.K., Dangott L.J., Li S., Kier A.B, & Schroeder F. (2016). FABP1: A NOVEL HEPATIC ENDOCANNABINOID AND CANNABINOID BINDING PROTEIN. Biochemistry, 55(37), 5243-5255.
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4

Comprehensive Cannabinoid Compound Acquisition

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The following compounds were purchased from Cayman Chemical: 4-fluoro MDMB-BUTINACA, 5-fluoro MDMB-PICA, AB-FUBINACA, AM1220, AM1248, JWH-018, JWH-122 N-(5-chloropentyl), MDMB-4en-PINACA, WIN 55,212-2, UR-144, XLR-11, anandamide (AEA) and JWH-133. Capsaicin was obtained from Sigma-Aldrich (St. Louis, MO). Controlled substances were purchased using the Walsh Laboratory DEA license.
Andersen H.K, & Walsh K.B. (2021). Molecular signaling of synthetic cannabinoids: Comparison of CB1 receptor and TRPV1 channel activation. European journal of pharmacology, 907, 174301.
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5

Pharmacokinetics of Synthetic Cannabinoid

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All chemicals used in this study were at least reagent grade. JWH-018 ((1-pentyl-1H-indol-3-yl)-1-naphthalenyl-methanone) was purchased from Cayman Chemical (Ann Arbor, MI). Unless otherwise specified, all chemicals and reagents were purchased from either Sigma-Aldrich (St. Louis, MO) or Thermo Fisher Scientific (Waltham, MA). Ethyl alcohol (100%) was purchased from AAPER (Shelbyville, KY). Recombinant CYP2C9*1, CYP2C9*2 and CYP2C9*3 were expressed in baculovirus-infected insect cells and purchased from BD Biosciences (San Jose, CA).
Patton A.L., Seely K.A., Yarbrough A.L., Fantegrossi W., James L.P., McCain K.R., Fujiwara R., Prather P.L., Moran J.H, & Radominska-Pandya A. (2018). Altered metabolism of synthetic cannabinoid JWH-018 by human cytochrome P450 2C9 and variants. Biochemical and biophysical research communications, 498(3), 597-602.
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6

Synthetic Cannabinoids and Drug Standards Extraction

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The standards of synthetic cannabinoids JWH 018, JWH 073, AM 1220 and WIN 55 were purchased from Cayman Chemical (Ann Arbor, MI, USA) at a concentration of 1 or 0.1 mg mL -1 based on availability. Standards of cocaine (COC), phencyclidine (PCP), morphine (MOR) and methamphetamine (MAMP) were purchased from LGC Standard (Italy). The purity of the reference compounds was > 99%. All standards were provided at a concentration of 3 mM. Individual stock solutions were prepared in methanol at 300 μM and working standard mixtures were prepared by appropriate dilution of the standard solutions in methanol. All solutions were stored at -20 °C in dark condition. All buffer reagents, methanol, acetonitrile and water were acquired from Fisher Scientific (Fair Lawn, NJ, USA). All solvents employed in the extraction were ultra-performance liquid chromatography (UPLC) grade, and LC-MS grade.
The solid phase extraction sorbent materials VYWLVW-resin (W) and YYIGGF-resin (F), with a peptide substitution level of 0.17 mmol g -1 and a synthesis reproducibility > 95%. were bought from EspiKem srl (Italy). The resin used to attach the hexapeptides was a Fmoc-PAL-AM. The C-18 cartridges (30 mg mL -1 ) were from Phenomenex. SPE Isolute column (Empty 1 mL Reservoir) was from STEPBIO (Italy).
Mascini M., Montesano C., Perez G., Wang J., Compagnone D, & Sergi M. (2017). Selective solid phase extraction of JWH synthetic cannabinoids by using computationally designed peptides. Talanta, 167.
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