Human bmp6

Manufactured by R&D Systems

Human BMP6 is a recombinant protein that belongs to the bone morphogenetic protein (BMP) family. BMPs are a group of growth factors that play a crucial role in the regulation of bone and cartilage formation. Human BMP6 is a secreted protein that can induce the formation of bone and cartilage.

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Lab products found in correlation

Anti phospho mek1 2, by Cell Signaling Technology (1 mentions) Ab51451, by Abcam (1 mentions) Ab75273, by Abcam (1 mentions) Human tgf β1, by R&D Systems (1 mentions) Sc 535, by Santa Cruz Biotechnology (1 mentions) Human fgf9, by R&D Systems (1 mentions) Human bmp4, by R&D Systems (1 mentions) Anti total p44 42 mapk, by Cell Signaling Technology (1 mentions) Anti phospho frs2 α tyr196, by Cell Signaling Technology (1 mentions) Sc 7267, by Santa Cruz Biotechnology (1 mentions) Anti phospho p38, by Cell Signaling Technology (1 mentions) Deferoxamine, by Merck Group (1 mentions) Human holo transferrin, by Merck Group (1 mentions) Human il 6, by Merck Group (1 mentions) Murine il 6, by Cell Signaling Technology (1 mentions) Follistatin, by R&D Systems (1 mentions) Insulin transferrin selenium its g, by Thermo Fisher Scientific (1 mentions) Forskolin fsk, by Merck Group (1 mentions) Noggin, by R&D Systems (1 mentions) Activin a, by R&D Systems (1 mentions)

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Recombinant human BMP6 (12 citations)

3 protocols using human bmp6

Investigating Intracellular Signaling Pathways

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Recombinant bovine FGF2, human FGF9, human TGFβ1, mouse noggin/Fc chimera, human BMP4, human BMP6, and anti-BMP antibody (MAB3552) were from R&D Systems (Minneapolis, MN). The following antibodies were all purchased from Cell Signaling Technology (Danvers, MA): anti–phospho-p44/42 MAPK E10 mouse monoclonal (#9106), anti–total p44/42 MAPK (#9102), anti–phospho-p38 (#9211), anti–phospho-MEK1/2 (#9154), anti–phospho Raf-1 (#9427), anti–phospho-FRS2-α(Tyr-196) (#3864), and anti–phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465) (#9511). Other antibodies used were as follows: for CP49, rabbit anti-mouse CP49 polyclonal serum (#899 or #900; both generous gifts of Paul FitzGerald, University of California, Davis, CA); for phospho-tyrosine, 4G10 (a kind gift from Brian Druker, Oregon Health and Science University, Portland, OR); for luciferase, #G745A from Promega (Madison, WI); for GFP, JL-8 from Clontech (Mountain View, CA); for phospho-Smad3, ab51451 from Abcam (Cambridge, MA); for total Smad 1/5, ab75273 from Abcam; for total Raf-1, sc-7267 from Santa Cruz Biotechnology (Santa Cruz, CA); for total p38, sc-535 from Santa Cruz; and for total MEK, M17030 from Transduction Labs (Lexington, KT). UO126 (used at 15 μM), PD173074 (100 nM), and dorsomorphin (5 μM) were from Calbiochem (La Jolla, CA). All other reagents, including TPA, were from Sigma-Aldrich (St. Louis, MO).

Boswell B.A, & Musil L.S. (2015). Synergistic interaction between the fibroblast growth factor and bone morphogenetic protein signaling pathways in lens cells. Molecular Biology of the Cell, 26(13), 2561-2572.

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Iron chelation and cytokine regulation

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Deferoxamine (DFO), FeSO₄ and ferric ammonium citrate (FAC) were purchased from Sigma-Aldrich. Following recombinant proteins were used: murine IL-6 (#5216, Cell Signaling), human IL-6 (#I1395, Sigma-Aldrich), human BMP6 (#507-BP, R&D Systems), and human holo-transferrin (#T4132, Sigma). Salicylaldehyde isonicotinoyl hydrazone (SIH) was a gift of the late Dr. Prem Ponka. Fe-SIH was prepared by mixing SIH with ferric citrate in 2:1 ratio [20 (link)].

Charlebois E, & Pantopoulos K. (2021). Iron overload inhibits BMP/SMAD and IL-6/STAT3 signaling to hepcidin in cultured hepatocytes. PLoS ONE, 16(6), e0253475.

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Adrenocortical Cell Culture Protocol

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Forskolin (FSK) was purchased from Sigma-Aldrich Co. Ltd. (St. Louis, MO) and recombinant proteins of human BMP-6, activin A, noggin and follistatin were purchased from R&D Systems Inc. (Minneapolis, MN). Human adrenocortical H295R cells, originating from a human adrenal gland carcinoma cell line, were cultured in DMEM/F12 containing 10% FBS and Insulin-Transferrin-Selenium (ITS-G; Thermo Fisher Scientific, Waltham, MA) with 5% CO 2 at 37°C.

Soejima Y., Iwata N., Nakano Y., Yamamoto K., Suyama A., Nada T., Ogawa H, & Otsuka F. (2021). Involvement of clock gene expression, bone morphogenetic protein and activin in adrenocortical steroidogenesis by human H295R cells. Endocrine journal, 68(2).

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