Log In Sign up
The largest database of trusted experimental protocols

0.33 cm2 transwell inserts

Manufactured by Corning
Visit Supplier
Sourced in United States

The 0.33 cm2 Transwell inserts are a specialized laboratory equipment designed for cell culture applications. These inserts provide a permeable membrane support for the growth and study of cells in a controlled environment. The inserts have a defined surface area of 0.33 cm2, which is a key specification for various experimental protocols.

Automatically generated - may contain errors

Lab products found in correlation

Nhbe cells, by Lonza (2 mentions) Mdck cells, by American Type Culture Collection (1 mentions) L glutamine, by Corning (1 mentions) Ham s f 12k medium, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions)

4 protocols using 0.33 cm2 transwell inserts

1

Quantifying Bacterial Transmigration in NPTr Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
NPTr cells were cultured on 0.33 cm2 Transwell inserts (Corning, New York, USA) with 3 μm pore size at a density of 7.5 × 104/well. The cells were grown to confluent monolayers and then incubated for another 5 d to allow cell polarization. The cells were infected in the apical compartment at an MOI of 2 with G. parasuis, P. multocida, or B. bronchiseptica, or at an MOI of 1 with A. pleuropneumoniae for 2 h and 5 h. After apical infection, the number of transmigrated bacteria was quantified in aliquots from the basal chambers and CFUs were counted on plates.
Cao Q., Wei W., Wang H., Wang Z., Lv Y., Dai M., Tan C., Chen H, & Wang X. (2021). Cleavage of E-cadherin by porcine respiratory bacterial pathogens facilitates airway epithelial barrier disruption and bacterial paracellular transmigration. Virulence, 12(1), 2296-2313.
+ Open protocol
+ Expand
2

Differentiated NHBE Cell Biofilm Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
NHBE cells (Lonza, Walkersville, MD, USA) were plated in 0.33 cm2 transwell inserts (Corning, Corning, NY, USA) at a seeding density of 1.5 × 104 cells per well and maintained in culture as previously described (Krunkosky et al., 2003 (link)). Briefly, cells were maintained in bronchial epithelial basal media (BEBM, Lonza) supplemented with the bronchial epithelial growth media (BEGM) BulletKit (Lonza) until confluent. An air/liquid interface (ALI) was established where the apical surface was exposed to a humidified 95% air/5% CO2 environment. Medium in the basolateral compartment was changed to ALI media (1:1 DMEM:BEBM) supplemented with BEGM Bullet Kit. The basolateral medium was changed every 2 days for a minimum of 5 weeks in culture to allow the cells to differentiate. For biofilm experiments, parental T4R (1 × 105/10 μL) were inoculated into the apical chamber directly into the mucous layer. Medium in the basolateral chamber was replaced with media containing 0, 100, and 500 μM zinc. Following 24 h incubation, transwell inserts were then prepped for SEM.
Brown L.R., Caulkins R.C., Schartel T.E., Rosch J.W., Honsa E.S., Schultz-Cherry S., Meliopoulos V.A., Cherry S, & Thornton J.A. (2017). Increased Zinc Availability Enhances Initial Aggregation and Biofilm Formation of Streptococcus pneumoniae. Frontiers in Cellular and Infection Microbiology, 7, 233.
+ Open protocol
+ Expand
3

MDCK and NHBE Cell Culture Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Madin-Darby canine kidney (MDCK) cells (ATCC, Manassas, VA, USA) were serially passaged and maintained in culture in modified Eagle's medium (CellGro, Herndon, VA, USA) supplemented with L-glutamine (2 mM), 5% fetal bovine serum and antibiotics at 37 °C with 5% CO2. Normal human bronchial epithelial (NHBE) cells (Lonza, Walkersville, MD, USA) from two healthy male donors (two and four years old) were expanded, cryopreserved and maintained in culture in an air/liquid interface (ALI) system, as previously described.27 (link), 28 (link) Briefly, cells were plated in 0.33 cm2 transwell inserts (Corning, Corning, NY, USA) and allowed to differentiate. An ALI was established when the cells reached 98%–100% confluence. Media from the apical surface of the cells was removed, and the cells were exposed to a humidified 95% air/5% CO2 environment. The basolateral media or the ALI media, which was supplemented with SingleQuot growth factors (Lonza), was changed every 48 h for a minimum of six weeks. During every media change, the apical surface of the cells was washed with sterile phosphate-buffered saline (PBS) to remove mucus.
Shanmuganatham K.K., Jones J.C., Marathe B.M., Feeroz M.M., Jones-Engel L., Walker D., Turner J., Rabiul Alam S.M., Kamrul Hasan M., Akhtar S., Seiler P., McKenzie P., Krauss S., Webby R.J, & Webster R.G. (2016). The replication of Bangladeshi H9N2 avian influenza viruses carrying genes from H7N3 in mammals. Emerging Microbes & Infections, 5(4), e35-.
+ Open protocol
+ Expand
4

Cell Culture and Virus Propagation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Madin-Darby canine kidney (MDCK) cells were grown in minimal essential medium (MEM) supplemented with 10% fetal calf serum, 100U penicillin/streptomycin, 2 μM L-glutamine, and vitamins (Gibco, NY). The quail-derived fibroblast cell line QT6 was grown in Ham’s F12K medium (Life Technologies, NY) supplemented with 5% fetal calf serum, 100U penicillin/streptomycin, and 10% tryptose phosphate broth. Chicken-origin DF-1 cells were grown in Dulbecco’s MEM that was similarly supplemented. Primary duck embryo fibroblast (DEF) cells were grown in supplemented, antibiotic-free MEM. All cells were grown at 37°C in a 5% CO2 environment.
Normalized human bronchial epithelial (NHBE) cells from a healthy male were purchased from Lonza (Walkersville, MD), plated in 0.33 cm2 Transwell inserts (Corning, NY) and allowed to differentiate in an air–liquid interface environment of 95% air and 5% CO2.
Passage 1 A/Quail/California/D113023808/2012 (Quail/CA12) was received from the University of California, Davis- and egg passage 2 A/Pekin Duck/California/P30/2006 (PD/CA06) was received from the University of Minnesota. Both viruses were propagated for an additional passage in embryonated chicken eggs.
Wong S.S., Yoon S.W., Zanin M., Song M.S., Oshansky C., Zaraket H., Sonnberg S., Rubrum A., Seiler P., Ferguson A., Krauss S., Cardona C., Webby R.J, & Crossley B. (2014). Characterization of an H4N2 Influenza Virus from Quails with a Multibasic Motif in the Hemagglutinin Cleavage Site. Virology, 0, 72-80.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!