Ap conjugated goat anti rabbit antibodies

Eugenol, Hank’s Balanced Salt Solution (HBSS), Phosphate Buffered Saline (PBS), Phorbolmyrisate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (fMLF), mouse monoclonal anti-β-actin antibody, phosphatase and proteases inhibitors were from Sigma Aldrich (Saint Quentin Falavier, France). Dextran T500 and Ficoll were purchased from GE healthcare (Orsay, France). SDS-PAGE and western blot reagents were from Bio-Rad Laboratories (Hercules, CA, USA). Anti-phospho-Raf, Anti-phospho-MEK1/2, Anti-phospho-p38 and Anti-phospho-ERK 1/2 antibodies were from cell signaling Technology (Boston, MA, USA). The anti-phospho-p47phox antibodies (Ser-328 and Ser-345) were generated by our lab as previously described^{35 (link)}. HRP-conjugated goat anti-rabbit, HRP-conjugated goat anti-mouse, AP-conjugated goat anti-rabbit antibodies and ECL (enhanced chemiluminescence) reagent were from Santa Cruz Biotechnology Inc (Heidelberg, Germany).

Juglone, PiB, cytochalasin B, Bovine alpha Chymotrypsin, Ortho-dianisidine dihydrochloride, LiCl, fMLP, Cytochrome C, PMA, protease and phosphatase inhibitors, buffers, and salt solutions were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). Polymorphprep is purchased from Axis-Shields (Oslo, Norway). Anti-MPO and anti-NGAL antibodies were from Abcam (Cambridge, UK). HRP-conjugated goat anti-rabbit, HRP-conjugated goat anti-mouse, AP-conjugated goat anti-rabbit antibodies, and luminol chemiluminescence reagents were from Santa Cruz Biotechnology Inc. (Heidelberg, Germany). Dextran T500 was from Pharmacosmos (Holbaek, Denmark). Ficoll was from GE Healthcare Bio-Sciences. SDS–PAGE and Western blotting reagents were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Pin1 peptide substrates were purchased from Bachem (Heidelberg, Germany).