Tsg101 14497 1 ap

Manufactured by Proteintech

TSG101 (14497-1-AP) is a rabbit polyclonal antibody from Proteintech. It recognizes the human Tumor Susceptibility Gene 101 (TSG101) protein.

Automatically generated - may contain errors

Lab products found in correlation

Exoq5a 1, by System Biosciences (2 mentions) F actin, by Yeasen (1 mentions) Gpx4 67763 1 ig, by Proteintech (1 mentions) Sorafenib, by MedChemExpress (1 mentions) Cd9 60232 1 ig, by Proteintech (1 mentions) Gapdh, by CWBIO (1 mentions) Ferrostatin 1, by MedChemExpress (1 mentions) Dhodh 14877 1 ap, by Proteintech (1 mentions) Calnexin, by Proteintech (1 mentions) Cd63 25682 1 ap, by Proteintech (1 mentions) Cd81 66866 1 ig, by Proteintech (1 mentions)

2 protocols using tsg101 14497 1 ap

Antibody-based Protein Detection and Inhibitor Assessment

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The antibodies used targeted the following proteins (dilutions used are included):
GAPDH (CW0101: immunoblotting, 1:1000) from CWBIOTECH; F-actin (40734ES75: immunofluorescence, 1:100) from YEASEN; U1A (10212: immunoblotting, 1:500) from Proteintech; CD63 (67605-1-Ig: immunoblotting, 1:5000) from Proteintech; CD9 (60232-1-Ig: immunoblotting, 1:5000) from Proteintech; TSG101 (14497-1-AP: immunoblotting, 1:1000) from Proteintech; GPX4 (67763-1-Ig: immunoblotting, 1:5000; IHC, 1:1000) from Proteintech; and DHODH (14877-1-AP: immunoblotting, 1:2000; IHC, 1:100) from Proteintech.
The inhibitors used are as follows:
Sorafenib (HY-10201: 10 μM for the in vitro assay and 30 mg/kg for the in vivo assay) and Ferrostatin-1 (HY-100579: 60 nM for the in vitro assay), and Ferrostatin-1 (HY-100579: 5 mg/kg, intraperitoneal injection for the in vivo assay) were obtained from MedChem Express.

Li X., Yu Q., Zhao R., Guo X., Liu C., Zhang K., Zhang W., Liu J., Yu J., Wang S., Hao Q., Li W., Zhang W., Li M., Zhang Y., Zhang C, & Gao Y. (2022). Designer Exosomes for Targeted Delivery of a Novel Therapeutic Cargo to Enhance Sorafenib-Mediated Ferroptosis in Hepatocellular Carcinoma. Frontiers in Oncology, 12, 898156.

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Exosome Isolation and Characterization from hASCs

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Exos were extracted from cell culture supernatant using an exo extraction kit (EXOQ5A-1, SBI) according to instructions. HASCs were cultured in DMEM, and the supernatant was collected at 48–72 h to extract exos for subsequent detection. The extracted exos were re-suspended in PBS in an appropriate volume. Particle size distribution of hASCs-Exos was measured by nanoparticle tracking analysis (NTA). The morphology and size of hASCs-Exos were displayed by transmission electron microscopy (TEM). Western blot analysis was performed using CD63 (25682-1-AP, 1:500, Proteintech), CD81(66866-1-Ig, 1:3000, Proteintech), TSG101 (14497-1-AP, 1:1000, Proteintech), GM130 (11308-1-AP, 1:5000, Proteintech) and Calnexin (10427-1-AP, 1:8000, Proteintech) and exosome uptake experiment was performed according to the previous study [27 (link)].

Chen D., Jiang X, & Zou H. (2023). hASCs-derived exosomal miR-155-5p targeting TGFβR2 promotes autophagy and reduces pyroptosis to alleviate intervertebral disc degeneration. Journal of Orthopaedic Translation, 39, 163-176.

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