MSCs or ECs were lysed using ice-cold lysis buffer containing 1% protease inhibitors and phosphatase inhibitors (Roche Applied Science, Penzberg, Germany). Proteins were fractionated by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis and then transferred to polyvinylidene difluoride (PVDF) membranes. Protein expression was detected by incubation with antibodies against CXCR4 (D1S7W; Cell Signaling Technology), phospho-VEGFR2 (bs-2674R; Bioss), integrin αvβ3 (bs-1310R; Bioss), p-FAK (bsm-52155R; Bioss), FAK (bs-20735R; Bioss), p-JAK2 (SY24-03; Novus), JAK2 (bs-23003R; Bioss), p-STAT3 (bs-1658R; Bioss), STAT3 (bsm-33218 M; Bioss), ATF3 (bs-0519R; Bioss), CHOP (bs-20669R; Bioss), p-ERK1/2 (D13.14.4E; Cell Signaling Technology), ERK1/2 (137F5; Cell Signaling Technology) or β-actin (AF0003; Beyotime).
Phospho vegfr2 bs 2674r
Manufactured by Bioss Antibodies
The Phospho-VEGFR2 (bs-2674R) is a lab equipment product offered by Bioss Antibodies. It is a phospho-specific antibody that recognizes the phosphorylated form of the Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) protein.
Automatically generated - may contain errors
Lab products found in correlation
Cxcr4 d1s7w, by Cell Signaling Technology (2 mentions)
Lsm 780, by Zeiss (2 mentions)
Stat3, by Bioss Antibodies (1 mentions)
Integrin αvβ3 bs1310r, by Bioss Antibodies (1 mentions)
P fak, by Bioss Antibodies (1 mentions)
Bs 23003r, by Bioss Antibodies (1 mentions)
Pstat3, by Bioss Antibodies (1 mentions)
Bs 1658r, by Bioss Antibodies (1 mentions)
Bsm 33218 m, by Bioss Antibodies (1 mentions)
Bs 0519r, by Bioss Antibodies (1 mentions)
P erk1 2 d13.14.4e, by Cell Signaling Technology (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
β actin, by Beyotime (1 mentions)
Protease inhibitor, by Roche (1 mentions)
Phosphatase inhibitor, by Roche (1 mentions)
Osteocalcin, by Bioss Antibodies (1 mentions)
Bs 1134r, by Bioss Antibodies (1 mentions)
Bs 1310r, by Bioss Antibodies (1 mentions)
Ab28364, by Abcam (1 mentions)
Endomucin v 7c7, by Santa Cruz Biotechnology (1 mentions)
2 protocols using phospho vegfr2 bs 2674r
1
Quantification of Signaling Pathway Proteins
2
Immunofluorescence Analysis of Bone Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunofluorescence was performed as previously described [28 (link),29 (link)]. Briefly, the bone sections were incubated with individual primary antibodies against mouse CD31 (ab28364; Abcam), endomucin (V.7C7; Santa Cruz), Ki67 (AF7617; R&D), beta-catenin (8480, CST), osterix (bs-1110R; Bioss), osteocalcin (bs-0470R; Bioss), Runx2 (bs-1134R; Bioss), DLL4 (bs-6044R; Bioss), Notch1 (bs-1335R; Bioss), Noggin (bs-2975R; Bioss), CXCR4 D1S7W; Cell Signaling Technology), integrin αvβ3 (bs-1310R; Bioss), CD90 (bs-20640R; Bioss), CD105 (bs-0579R; Bioss), CD271 (bs-0161R; Bioss), OPN (bs-23258R; Bioss), and phospho-VEGFR2(bs-2674R; Bioss) overnight at 4 °C. Subsequently, the samples were incubated with fluorescence-coupled secondary antibodies for 1 h at 37 °C in the dark. Images were collected using a confocal laser microscope (LSM 780; Carl Zeiss).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!