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The Z-LIG is a versatile laboratory equipment used for the analysis and detection of biological samples. It serves as a core component in various research and analytical applications. The device's primary function is to provide reliable and precise measurements through the utilization of advanced technological principles. No further information is available on the intended use or specific details of the product.

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2 protocols using z lig

1

Antibody Procurement for Molecular Studies

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Z-LIG with purity more than 98 % was purchased from Chengdu Must Bio-Technology Co, Ltd (Chengdu, China) and stored in −80 °C before use. The antibodies against BRCA1, ERα, HDAC1, HDAC2, HDAC4/5/7, Acetyl-Histone3(lys9/14), IFI16, and MTA1 were purchased from Santa Cruz Biotechnology (CA, USA). Acetyl-p53, pro-PARP and cleaved PARP antibody were obtained from Cell Signaling Technology (Boston, MA, USA). The antibodies against Cyclin A, Cyclin E, CDK1, CDK2, p53, Histone 3 were obtained from Wanlei Biotechnology (Shenyang, China). The antibodies against caspase 3, cleaved caspase 3, p21 and p27 were purchased from Proteintech Group Inc (Wuhan, China). The antibodies against β-actin and rabbit IgG were obtained from Sigma-Aldrich (St. Louis, MO, USA). Other chemicals were obtained from Sigma-Aldrich, unless indicated otherwise.
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2

Cancer Signaling Pathway Antibodies Protocol

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Z-LIG with purity more than 98% was obtained from Chengdu Must Bio-Technology Co, Ltd (Chengdu, China) and stored in −80 °C before use. The antibodies against BRCA1, Nur77, Bcl-2 and p62 were purchased from Santa Cruz Biotechnology (CA, USA). PI3KC3 and PARP antibodies were obtained from Cell Signaling Technology (Boston, MA, USA). The antibodies against CTSB and CTSD were obtained from Wanlei Biotechnology (Shenyang, China). The antibodies against ATG14, Beclin1, Ku70/80 and DNA-PKcs were purchased from Proteintech Group Inc (Wuhan, China). The antibodies against γ-H2AX and Rad51 were obtained from Bioss Biotechnology (Beijing, China). The antibodies against LC3, β-actin and rabbit IgG were obtained from Sigma-Aldrich (St. Louis, MO, USA). Other chemicals were obtained from Sigma-Aldrich, unless indicated otherwise.
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