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Ha ub k0

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HA-Ub-K0 is a recombinant protein that contains an N-terminal hemagglutinin (HA) tag and a ubiquitin (Ub) sequence with all lysine residues mutated to arginine (K0). This product is commonly used in research applications to study ubiquitin-related cellular processes.

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Lab products found in correlation

Quikchange lightning multi site directed mutagenesis kit, by Agilent Technologies (2 mentions) Expressed sequence tags, by GE Healthcare (2 mentions) Flag atm wt 31985, by Addgene (2 mentions)

Spelling variants of this product

HA-Ub (16 citations) PRK5-HA-Ub-K0 (2 citations)

3 protocols using ha ub k0

1

Cloning and Mutagenesis of hPEX5 and ATM

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hPEX5 is carried out by forward primer (5’-CGGTCGACCATGGCAATGCGGGAG-3’) and reverse primer (5’-GTCGACTCACTGGGGCAGGCCAAAC-3’) which include SalI restriction sites, to get the full-length hPex5 and ESTs clone as template (Expressed-sequence tags, GE Healthcare). hPEX5 PCR product cloned into pJET1.2/blunt cloning vector to amplify full-length hPex5. hPex5 is cut out by SalI from pJET1.2/blunt cloning vector insert it into pCMV-Myc expression vector. A series of mutant constructs of PEX5 were generated by QuikChange Lightning Multi Site-Directed mutagenesis Kit. The primers for different sites were designed as follow.
Flag-ATM-WT (#31985) and HA-p62 (#28027) plasmids were purchased from Addgene. Flag-ATM R3047Q mutant constructs was generated by QuikChange Lightning Multi Site-Directed mutagenesis Kit (Agilent Technologies).
HA-Ub (#18712) and HA-Ub-K0 (#17603) plasmids were purchased from Addgene. DsRed-PTS1 was provided by Dr. Michael Mancini (Baylor College of Medicine, USA). pAT-003 mRFP-EGFP-SKL was provided by Dr. Suresh Subramani (University of California, San Diego, USA). Specificity of mutagenesis was confirmed by direct sequencing using the Sequencing and Microarray Core at U.T. M.D. Anderson Cancer Center, Houston, Texas.
Zhang J., Tripathi D.N., Jing J., Alexander A., Kim J., Powell R.T., Dere R., Tait-Mulder J., Lee J.H., Paull T.T., Pandita R.K., Charaka V.K., Pandita T.K., Kastan M.B, & Walker C.L. (2015). ATM Functions at the Peroxisome to Induce Pexophagy in Response to ROS. Nature cell biology, 17(10), 1259-1269.
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2

Cloning and Mutagenesis of hPEX5 and ATM

Check if the same lab product or an alternative is used in the 5 most similar protocols
hPEX5 is carried out by forward primer (5’-CGGTCGACCATGGCAATGCGGGAG-3’) and reverse primer (5’-GTCGACTCACTGGGGCAGGCCAAAC-3’) which include SalI restriction sites, to get the full-length hPex5 and ESTs clone as template (Expressed-sequence tags, GE Healthcare). hPEX5 PCR product cloned into pJET1.2/blunt cloning vector to amplify full-length hPex5. hPex5 is cut out by SalI from pJET1.2/blunt cloning vector insert it into pCMV-Myc expression vector. A series of mutant constructs of PEX5 were generated by QuikChange Lightning Multi Site-Directed mutagenesis Kit. The primers for different sites were designed as follow.
Flag-ATM-WT (#31985) and HA-p62 (#28027) plasmids were purchased from Addgene. Flag-ATM R3047Q mutant constructs was generated by QuikChange Lightning Multi Site-Directed mutagenesis Kit (Agilent Technologies).
HA-Ub (#18712) and HA-Ub-K0 (#17603) plasmids were purchased from Addgene. DsRed-PTS1 was provided by Dr. Michael Mancini (Baylor College of Medicine, USA). pAT-003 mRFP-EGFP-SKL was provided by Dr. Suresh Subramani (University of California, San Diego, USA). Specificity of mutagenesis was confirmed by direct sequencing using the Sequencing and Microarray Core at U.T. M.D. Anderson Cancer Center, Houston, Texas.
Zhang J., Tripathi D.N., Jing J., Alexander A., Kim J., Powell R.T., Dere R., Tait-Mulder J., Lee J.H., Paull T.T., Pandita R.K., Charaka V.K., Pandita T.K., Kastan M.B, & Walker C.L. (2015). ATM Functions at the Peroxisome to Induce Pexophagy in Response to ROS. Nature cell biology, 17(10), 1259-1269.
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3

Drp1 and p53 Truncation Mutant Plasmids

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Full-length Drp1, FLAG–p53, GFP–p53, HA–ubiquitin (wild-type), HA–Ub K0 (ubiquitin lacking lysine residues), HA–ubiquitin (Lys48-linked) and ubiquitin (Lys63-linked) plasmids were obtained from Addgene. Truncating mutations of Drp1 were created by inserting PCR-amplified fragments into the pCMV-Myc vector. Myc–p53 truncated mutants were gifts from Dr Lingqiang Zhang (Beijing Institute of Radiation Medicine, Beijing, China).
Guo X., Sesaki H, & Qi X. (2014). Drp1 stabilizes p53 on the mitochondria to trigger necrosis under oxidative stress conditions in vitro and in vivo. The Biochemical journal, 461(1), 137-146.
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