Purified exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturers protocol. To check the yield, quality and size of extracted total RNA we analysed samples with an Agilent 2100 Bioanalyser (Agilent Technologies, United States) on a Eukaryote Total RNA chip.
Plasma serum circulating exosomal rna purification mini kit
Manufactured by Norgen Biotek
The Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit is a laboratory equipment designed to purify total RNA, including circulating and exosomal RNA, from plasma or serum samples.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 2100 bioanalyser, by Agilent Technologies (1 mentions)
Quant it ribogreen rna assay kit, by Thermo Fisher Scientific (1 mentions)
Rna 6000 pico chip, by Agilent Technologies (1 mentions)
Hiseq 2000 system, by Illumina (1 mentions)
Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions)
3 protocols using plasma serum circulating exosomal rna purification mini kit
1
Exosomal RNA Extraction and Analysis
2
Extracellular Vesicle RNA Extraction
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Purified EVs were extracted from the CM from both groups (blastocyst and nonblastocyst) by qEV single size exclusion column. As detailed above (illustrated in SI Appendix, Fig. S9B ), 600 µL (× 6 replicates) of the concentrated EV pellet from each group was used for total RNA extraction, using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer's protocol. The quality and concentration of the RNA samples were examined using an RNA 6000 Pico Chip (Agilent Technologies) and a Quant-iT RiboGreen RNA Assay kit (Life Technologies), respectively.
3
Exosomal RNA Extraction and Sequencing
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Serum exosomes were processed for RNA extraction using the Plasma/Serum Circulating & Exosomal RNA Purification Mini Kit (Norgen Biotek, Cat. 51000) according to the manufacturer’s protocol. Extracted total RNA samples were analyzed with a Eukaryote Total RNA chip on an Agilent 2100 Bioanalyzer (Agilent Technologies, United States) to confirm sufficient yield, quality, and size of RNA. Exosome RNA sequencing libraries were then constructed using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (BioLabs, New England) according to the manufacturer’s instructions. Yield and size distribution of resultant libraries were validated using Agilent 2100 Bioanalyzer on a High-sensitivity DNA Assay (Agilent Technologies, United States). Libraries were then pooled with an equal proportion for multiplexed sequencing on Illumina HiSeq. 2000 System at the Ramaciotti Centre for Genomics.
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