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Alexa fluor 700 mouse anti human cd3

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Alexa Fluor 700 mouse anti-human CD3 is a fluorescently labeled antibody that binds to the CD3 antigen expressed on human T cells. It can be used for the detection and identification of T cells in flow cytometry applications.

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Lab products found in correlation

Myristoyl chloride, by Merck Group (3 mentions) Fitc mouse anti human cd45, by BD (3 mentions) Apc mouse anti human cd4, by BD (3 mentions) Bv421 mouse anti human cd8, by BD (3 mentions) Polymer based hrp conjugated anti mouse envision secondary, by Agilent Technologies (3 mentions) 3 3 diaminobenzidine (dab), by Merck Group (3 mentions) Ciprofloxacin, by Merck Group (3 mentions) Paraformaldehyde pfa, by Merck Group (3 mentions) Gentamicin, by Thermo Fisher Scientific (3 mentions) Poloxamer 407 p407 n n diisopropylethylamine diea, by Merck Group (2 mentions) Hplc grade acetonitrile acn, by Thermo Fisher Scientific (2 mentions) Optima lc ms grade water, by Thermo Fisher Scientific (2 mentions) Monoclonal mouse anti human hiv 1p24 clone kal 1, by Agilent Technologies (2 mentions) Monoclonal mouse anti human leukocyte antigen hla dr clone cr3 43, by Agilent Technologies (2 mentions) Hplc grade methanol, by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle s medium dmem, by Corning (2 mentions) Fitc mouse anti human cd4, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Goat anti rabbit igg h l highly cross adsorbed secondary antibody, by Thermo Fisher Scientific (1 mentions) Diethyl ether, by Thermo Fisher Scientific (1 mentions)

5 protocols using alexa fluor 700 mouse anti human cd3

1

Optimizing HIV-1 Virus Detection Assay

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Native CAB and CAB LAP (200 mg/mL) were graciously obtained from ViiV Healthcare (Research Triangle Park, NC, USA). Myristoyl chloride, poloxamer 407 (P407), N,N-diisopropylethylamine (DIEA), dimethylformamide (DMF), 1-octanal, ciprofloxacin, paraformaldehyde (PFA), and 3,3′-diaminobenzidine (DAB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from Corning Life Sciences (Tewksbury, MA, USA). Heat-inactivated pooled human serum was purchased from Innovative Biologics (Herndon, VA, USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. (Rockville, MD, USA). Gentamicin, HPLC grade acetonitrile (ACN), HPLC grade methanol, and Optima grade LC/MS water was purchased from ThermoFisher Scientific (Waltham, MA, USA). FITC mouse anti-human CD45, Alexa Fluor 700 mouse anti-human CD3, APC mouse anti-human CD4, and BV421 mouse anti-human CD8 were purchased from BD Biosciences (San Jose, CA, USA). Monoclonal mouse anti-human HIV-1p24 (clone Kal-1), monoclonal mouse anti-human leukocyte antigen (HLA-DR; clone CR3/43), and the polymer-based HRP-conjugated anti-mouse EnVision + secondary were purchased from Dako (Carpinteria, CA, USA).
Zhou T., Su H., Dash P., Lin Z., Shetty B.L., Kocher T., Szlachetka A., Lamberty B., Fox H.S., Poluektova L., Gorantla S., McMillan J., Gautam N., Mosley R.L., Alnouti Y., Edagwa B, & Gendelman H.E. (2017). Creation of a nanoformulated cabotegravir prodrug with improved antiretroviral profiles. Biomaterials, 151, 53-65.
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2

Multiparameter Flow Cytometry Analysis

Cited 1 time
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PBMCs were stained for cell subsets using the following antibodies: Alexa Fluor 700 mouse anti-human CD3 (BD Pharmingen™/Clone: SP34–2), FITC mouse anti-human CD4 (BD Pharmingen™/Clone: OKT4), Pacific Blue mouse anti-human CD8 (BD Pharmingen™/Clone: RPA-T8), BUV805 mouse anti-human CD14 (BD Horizon™/Clone: M5E2), BUV 395 mouse anti-human CD19 (BD Horizon™/Clone: SJ25C1), APC anti-Hu CD56 (NCAM) (Invitrogen/Clone: TULY56). The mouse monoclonal anti-TRAILshort-specific antibody was developed in the Mayo Antibody Core facility as previously described (30 (link)). Intracellular staining for active caspase 3 was performed as described (33 (link)). Additional antibodies include mouse anti-TRAIL-R1 (B-N36 PE Cell Sciences) and mouse anti-TRAIL-R2 (B-K29 PE Cell Sciences). Flow cytometry was performed on BD LSR Fortessa flow cytometer. The results were analyzed with FlowJo v10.2 software (Tree Star, Inc, Ashland OR).
Natesampillai S., Paim A.C., Cummins N.W., Chandrasekar A.P., Bren G.D., Lewin S.R., Kiem H.P, & Badley A.D. (2019). TRAILshort protects against CD4 T cell death during acute HIV infection.. Journal of immunology (Baltimore, Md. : 1950), 203(3), 718-724.
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3

Optimizing HIV-1 Virus Detection Assay

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Native CAB and CAB LAP (200 mg/mL) were graciously obtained from ViiV Healthcare (Research Triangle Park, NC, USA). Myristoyl chloride, poloxamer 407 (P407), N,N-diisopropylethylamine (DIEA), dimethylformamide (DMF), 1-octanal, ciprofloxacin, paraformaldehyde (PFA), and 3,3′-diaminobenzidine (DAB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from Corning Life Sciences (Tewksbury, MA, USA). Heat-inactivated pooled human serum was purchased from Innovative Biologics (Herndon, VA, USA). Cell counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. (Rockville, MD, USA). Gentamicin, HPLC grade acetonitrile (ACN), HPLC grade methanol, and Optima grade LC/MS water was purchased from ThermoFisher Scientific (Waltham, MA, USA). FITC mouse anti-human CD45, Alexa Fluor 700 mouse anti-human CD3, APC mouse anti-human CD4, and BV421 mouse anti-human CD8 were purchased from BD Biosciences (San Jose, CA, USA). Monoclonal mouse anti-human HIV-1p24 (clone Kal-1), monoclonal mouse anti-human leukocyte antigen (HLA-DR; clone CR3/43), and the polymer-based HRP-conjugated anti-mouse EnVision + secondary were purchased from Dako (Carpinteria, CA, USA).
Zhou T., Su H., Dash P., Lin Z., Shetty B.L., Kocher T., Szlachetka A., Lamberty B., Fox H.S., Poluektova L., Gorantla S., McMillan J., Gautam N., Mosley R.L., Alnouti Y., Edagwa B, & Gendelman H.E. (2017). Creation of a nanoformulated cabotegravir prodrug with improved antiretroviral profiles. Biomaterials, 151, 53-65.
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4

Antibodies for Immunophenotyping Analysis

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The antibodies used in this study included mouse monoclonal anti-CD19 (Cat. AF0093, Beyotime, China), mouse monoclonal biotinylated anti-CD19 (Cat. 302204, Biolegend, USA), rabbit monoclonal recombinant anti-BCMA (Cat. ab253242, Abcam, USA), donkey anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody (Cat. A32766, Invitrogen, USA), goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody (Cat. A-11036, Invitrogen, USA) and Alexa Fluor® 700 mouse anti-Human CD3 (Cat. 557943, BD Pharmingen™, USA).
Liu Y., Zheng Y., Deng T., Huang Y., Liu Z., Zhan B., Zhou X., Yan R., Ren J., Xing Y., Wu G., Zheng B., Hu G., Wang W., Liu Y., Zhao J., Chen X, & Zhou G.G. (2022). Oncolytic herpes simplex virus delivery of dual CAR targets of CD19 and BCMA as well as immunomodulators to enhance therapeutic efficacy in solid tumors combined with CAR T cell therapy. Frontiers in Oncology, 12, 1037934.
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5

Characterization of Nanoformulated HIV Therapy

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DTG was a generous gift from ViiV Healthcare (Research Triangle Park, NC). Pyridine, dimethylformamide (DMF), N,N-diisopropylethylamine (DIEA), myristoyl chloride, poloxamer 407 (P407), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, ciprofloxacin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), paraformaldehyde (PFA), and 3,3’-diaminobenzidine (DAB) were purchased from Sigma-Aldrich (St. Louis, MO). Diethyl ether, cell culture grade water (endotoxin-free), gentamicin, acetonitrile (ACN), methanol, KH2PO4, bovine serum albumin (BSA), Triton X-100, LC-MS-grade water, and TRIzol reagent were purchased from Fisher Scientific (Hampton, NH). FITC mouse anti-human CD45, Alexa Fluor 700 mouse anti-human CD3, APC mouse anti-human CD4, BV421 mouse anti-human CD8, PE mouse anti-human CD14, and PE-Cy5 mouse anti-human CD19 were purchased from BD Biosciences (San Jose, CA). Monoclonal mouse anti-human HIV-1p24 (clone Kal-1), monoclonal mouse anti-human leukocyte antigen (HLA-DP/DQ/DR; clone CR3/43), and the polymer-based HRP-conjugated anti-mouse EnVision+ secondary were purchased from Dako (Carpinteria, CA). Heat-inactivated pooled human serum was purchased from Innovative Biologics (Herndon, VA). Dulbecco’s Modification of Eagle’s Medium (DMEM) was purchased from Corning Life Sciences (Tewksbury, MA).
Sillman B., Bade A.N., Dash P.K., Bhargavan B., Kocher T., Mathews S., Su H., Kanmogne G.D., Poluektova L.Y., Gorantla S., McMillan J., Gautam N., Alnouti Y., Edagwa B, & Gendelman H.E. (2018). Creation of a long-acting nanoformulated dolutegravir. Nature Communications, 9, 443.
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