The antioxidant activity of NOEO was measured by ABTS assay according to the protocols of an ABTS kit (S0119, Beyotime Biotechnology, Shanghai, China). The absorbance at 734 nm was then read in a microplate reader (Synergy H1MF, BioTek, Winooski, VT, USA). The results are expressed as the mean ± standard deviation.
Abts kit
Manufactured by Beyotime
Sourced in China
The ABTS kit is a laboratory reagent used for the colorimetric determination of antioxidant activity. It provides a quick and reliable method to measure the total antioxidant capacity of a sample.
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Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Synergy h1mf, by Agilent Technologies (1 mentions)
Dichloromethane, by Sinopharm (1 mentions)
Ethanol, by Sinopharm (1 mentions)
Infinite e plex, by Tecan (1 mentions)
Blue dextran, by Merck Group (1 mentions)
2 2 azobis 2 amidinopropane hydrochloride, by Merck Group (1 mentions)
Bromophenol blue, by Merck Group (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt, by Merck Group (1 mentions)
Sepharose cl 4b column, by Sinopharm (1 mentions)
Dcfh da, by Merck Group (1 mentions)
Milli q water system, by Merck Group (1 mentions)
Flexstation 3, by Molecular Devices (1 mentions)
5 protocols using abts kit
1
ABTS Assay for NOEO Antioxidant Activity
2
Antioxidant Potential of Coreopsis tinctoria
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Coreopsis tinctoria Nutt. (originated from Hetian, Xinjiang, batch number: 20190617-KLXJ) was purchased from Tongxiang Haitai Juye Co., Ltd. The DPPH kit was purchased from Sangon Biotech (Shanghai) Co., Ltd. The ABTS kit was bought from Beyotime Institute of Biotechnology, China. Dichloromethane and ethanol (analytical grade) were purchased from Sinopharm Chemical Reagent Co., Ltd, China.
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Coreopsis tinctoria Nutt. (originated from Hetian, Xinjiang, batch number: 20190617-KLXJ) was purchased from Tongxiang Haitai Juye Co., Ltd. The DPPH kit was purchased from Sangon Biotech (Shanghai) Co., Ltd. The ABTS kit was bought from Beyotime Institute of Biotechnology, China. Dichloromethane and ethanol (analytical grade) were purchased from Sinopharm Chemical Reagent Co., Ltd, China.
3
ABTS Radical Scavenging Activity Assay
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The ABTS solution from the ABTS kit (S0119, Beyotime, Nantong, China) was mixed with the oxidant master mix in equal proportions and stored for 12 h before use, and 400 μL of the master mix was mixed with 13.6 mL of 0.01 M phosphate buffer (pH = 7.3, C0221A, Beyotime, Nantong, China) as the working master mix. Ten microliters of the sample solution containing different samples at a concentration of 0.5 mg/mL was added to 200 μL of working master batch and reacted for 5 min to measure at 734 nm using an ultraviolet-visible spectrophotometer (Infinite E Plex, TECAN, Männedorf, Switzerland). Trolox ((±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid) was used to compare the ABTS radical scavenging activity with the TPs, and the results would be shown as mg Trolox equivalents (TE)/g TPs [20 (link)].
4
Rice Vinegar Characterization and Antioxidant Assays
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Vinegar was provided by Jiangsu Hengshun Vinegar-Industry Co., Ltd (Jiangshu, China), which is one of the representative rice vinegars in China. Deionized water was prepared by a Millipore Milli-Q water system (Millipore, Bedford, MA, USA). Blue Dextran (MW: ~500 kDa), and Bromophenol blue (MW: ~670 Da), 2,2-azobis(2-amidinopropane) hydrochloride (AAPH), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and DCFH-DA ( ≥ 97% purity) were purchased from Sigma-Aldrich Co., Ltd. (Shanghai, China). ABTS kit was purchased from Beyotime Biotechnology Co., Ltd (Shanghai, China). DMEM, FBS, streptomycin, PBS and penicillin were purchased from Thermo Fisher Scientific (China) Co., Ltd (Shanghai, China). All the chemicals and solvents, unless otherwise noted, used were of AR grade, and Sepharose CL-4B column (60–2 × 104 kDa) were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
5
Carnosol Antioxidant Activity Assay
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Carnosol was dissolved in DMSO at a concentration of 40 mM for stock solution. Antioxidant activity detected by ABTS kit (Beyotime, Shanghai, China). The ABTS solution mixed with K2S2O8 to form a work solution and was stored at room temperature for 12 h–16 h before being used. Then the ABTS work solution was diluted with PBS for 40 times. 20 μL of carnosol at various concentration (10 ~ 0.04 μM) was mixed with 180 μL of diluted ABTS work solution. The absorbance at 734 nm was measured after incubation for 2–6 min at room temperature. We use the Absorbance (ODs) function of Flex station 3 (Molecular Devices, San Francisco, CA, USA) to detect the absorbance.
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