γ glucys

Manufactured by Merck Group

Sourced in United States, Germany

γ-GluCys is a laboratory product offered by the Merck Group. It functions as a biochemical reagent for use in research and analysis applications.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using γ glucys

Quantification of Thiol Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

l-Cys, D,l-Hcy, l-GSH, CysGly, and γGluCys were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-Acetyl-l-Cysteine (NAC), tiopronin (N-(2-mercaptopropionyl)glycine, MPG), and trichloroacetic acid (TCA) were obtained from Wako (Osaka, Japan). Ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) was purchased from Dojindo (Kumamoto, Japan). TCEP was from Tokyo Chemical Industry (Tokyo, Japan). Phosphate buffered saline (PBS) was purchased from Takara Bio (Shiga, Japan). HPLC-grade acetonitrile was used. Water was purified using a Milli-Q system (Millipore, Bedford, MA, USA). All other chemicals were of analytical grade.

Chang C., Isokawa M., Funatsu T, & Tsunoda M. (2019). Optimization of tris(2-carboxyethyl) phosphine reduction conditions for fast analysis of total biothiols in mouse serum samples. Heliyon, 5(5), e01598.

+ Open protocol

+ Expand

Quantification of Glutathione Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

GSH, GSSG, γ-Glu-Cys, Cys-Gly, 5-oxo-proline, Glu and the other amino acids used in the study, CH₃OH, CD₃OD (99.8 atom % ²H), acetone, pentafluoropropionic anhydride, 2,3,4,5,6-pentafluorobenzyl bromide, ophthalmic acid (γ-glutamyl-α-amino-n-butyryl-glycine) and borax (for the preparation of borate buffer) were purchased from Sigma-Aldrich (Steinheim, Germany). Hydrochloric acid (ultrapure, 37%) was from AppliChem (Darmstadt, Germany). Stock solutions were prepared in, and diluted with, deionized water, as appropriate. Glassware for GC-MS (1.8-mL autosampler vials and 0.2-mL microvials) were purchased from Macherey-Nagel (Düren, Germany).
Safety Considerations. PFPA is corrosive and malodorous. PFB-Br is corrosive and a lachrymator. Inhalation and contact with skin and eyes should be avoided. All work should be, and was, performed in a well-ventilated fume hood.

Bollenbach A, & Tsikas D. (2022). GC-MS Studies on the Conversion and Derivatization of γ-Glutamyl Peptides to Pyroglutamate (5-Oxo-Proline) Methyl Ester Pentafluoropropione Amide Derivatives. Molecules, 27(18), 6020.

+ Open protocol

+ Expand

Glutathione and Cysteine Derivatives Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Glutathione was purchased from Alfa Aesar (Ward Hill, MA USA). Cysteine, ammonium formate, and 2,3-dimethoxy-5-methyl-1,4-benzoquinone (BQ) were obtained from Acros Organic (Geel, Belgium). HomoCysteine, penicillamine, cys-gly, γ-glu-cys, N-cyclohexylmaleimide and N-tert-butylmaleimide were purchased from Sigma Aldrich (Saint Louis, MO USA). Formic acid and ammonium hydroxide were obtained from Fisher Scientific (Pittsburgh, PA USA). LC-MS grade water and acetonitrile were from Honeywell Burdick and Jackson (Muskegon, MI USA).

Zhao X., Hui D.S., Lee R, & Edwards J.L. (2018). Ratiometric Quantitation of Thiol Metabolites using Non-Isotopic Mass Tags. Analytica chimica acta, 1037, 274-280.

+ Open protocol

+ Expand

Kinetic Analysis of Glutathione Gamma-Glutamyl Peptidases

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kinetic parameters were determined using 0.5 μg of GGP1, 0.25 μg of GGP3, or 2.2 μg of GGCT2;3. The proteins were incubated in 50 μl of reaction mixture containing 50 mm Tris–HCl (pH 8.0) and 0.25 to 15.0 mm GSH for 30 min at 37°C, and the reaction was terminated by adding 10 μl of 1.5 m HCl. The solution was centrifuged at 11 000 g for 15 min at 4°C, and the Cys‐Gly released in the supernatant was quantified by HPLC, as described below. Kinetic parameters were calculated using GraphPad Prism 9 software (GraphPad Software, San Diego, CA, USA). For degradation activity for other γ‐glutamyl compounds, 0.5 μg of GGP1 or 0.25 μg of GGP3 was incubated in 50 μl of reaction mixture containing 50 mm Tris–HCl (pH 8.0) and 5.0 mm GSH, 5.0 mm γ‐Glu‐Cys (Sigma‐Aldrich), 5.0 mm γ‐Glu‐Ala (Sigma‐Aldrich, St. Louis, MO, USA), or 2.5 mm GSSG. The reaction was conducted as described previously. As described below, the released Cys‐Gly was quantified for GSH, Cys was quantified for γ‐Glu‐Cys, and Ala was quantified for γ‐Glu‐Ala.

Ito T., Kitaiwa T., Nishizono K., Umahashi M., Miyaji S., Agake S., Kuwahara K., Yokoyama T., Fushinobu S., Maruyama‐Nakashita A., Sugiyama R., Sato M., Inaba J., Hirai M.Y, & Ohkama‐Ohtsu N. (2022). Glutathione degradation activity of γ‐glutamyl peptidase 1 manifests its dual roles in primary and secondary sulfur metabolism in Arabidopsis. The Plant Journal, 111(6), 1626-1642.

+ Open protocol

+ Expand

Intracellular GSH Quantification Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells (40,000 per well, duplicate per condition) were seeded in 96-well plates at 37 °C/5% CO₂ in DMEM supplemented or not with different compounds (3 mM GSH, 10 mM GSH, 3 mM GSH + 100 μM β-ME, 3 mM GSH ethylester (GSHEE, Sigma-Aldrich, St. Louis, CA, USA), 1 mM N-acetylcysteine (NAC, Sigma-Aldrich, St. Louis, CA, USA), 150 μM gamma-glutamylcysteine (γ-GluCys, Sigma-Aldrich, St. Louis, CA, USA)). Intracellular GSH level was determined after 24 and 48 h by SAFAS Xenius XOF (Safas) using the GSH fluorimetric assay kit (CS1020, Sigma-Aldrich, St. Louis, CA, USA) according to the manufacturer’s instructions. Relative GSH content was normalized to the number of cells.

Daher B., Meira W., Durivault J., Gotorbe C., Pouyssegur J, & Vucetic M. (2022). Genetic Disruption of the γ-Glutamylcysteine Ligase in PDAC Cells Induces Ferroptosis-Independent Cell Death In Vitro without Affecting In Vivo Tumor Growth. Cancers, 14(13), 3154.

+ Open protocol

+ Expand

Quantification of Deoxynivalenol Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

DON (≥98%), GSH (≥98%), Cys, γ-GluCys, CysGly, NAC and Na₂CO₃ (pro analysis) were from Sigma-Aldrich (Steinheim, Germany), and NaHCO₃ (pro analysis) was from Fluka (Steinheim, Germany). NaHCO₃ and Na₂CO₃ were used to prepare 0.2 M buffer with pH of 10.7 as measured with a Mettler Delta 320 pH meter at ambient temperature. Purified quantitative standards of DON-10-Cys, DON-10-GSH, DON-13-Cys and DON-13-GSH were available from previous work [6 (link),7 (link)], while DON-3-O-β-d-glucoside, DON-3-O-acetate and DON-15-O-acetate were from Romer Labs (Tulln, Austria).

Uhlig S., Stanic A., Hofgaard I.S., Kluger B., Schuhmacher R, & Miles C.O. (2016). Glutathione-Conjugates of Deoxynivalenol in Naturally Contaminated Grain Are Primarily Linked via the Epoxide Group. Toxins, 8(11), 329.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!