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Biotin peg thiol

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Biotin-PEG-thiol is a versatile linker molecule used in various bioconjugation applications. It contains a biotin group, a polyethylene glycol (PEG) spacer, and a thiol group. The biotin moiety can be used for specific binding to avidin or streptavidin, while the thiol group enables covalent attachment to surfaces or other biomolecules.

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Lab products found in correlation

Mineral oil, by Merck Group (2 mentions) Peg 4mal, by Laysan Bio (2 mentions) Hts 7000, by PerkinElmer (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Dithiothreitol (dtt), by Merck Group (2 mentions) Span 80, by Merck Group (2 mentions) Paraformaldehyde (pfa), by Avantor (1 mentions) Polystyrene beads, by Merck Group (1 mentions) Micro cover glass, by Avantor (1 mentions) Dulbecco s phosphate buffered saline, by Avantor (1 mentions) Milli q gradient water purification system, by Merck Group (1 mentions) Peg thiol, by Creative PEGWorks (1 mentions) Tris 2 carboxyethyl phosphine hydrochloride, by Thermo Fisher Scientific (1 mentions) Neutravidin, by Thermo Fisher Scientific (1 mentions) Hydrogen peroxide, by Merck Group (1 mentions) Sulfuric acid, by Merck Group (1 mentions) Ethyl alcohol, by Merck Group (1 mentions)

3 protocols using biotin peg thiol

1

PEG-based Microgel Synthesis with Biotin

Cited 1 time
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A microgel precursor solution containing 5% w/v PEG-4MAL (20kDa, Laysan Bio) and 1.0 mM biotin-PEG-thiol (1 kDa, Nanocs) was reacted for 15 min in PBS. This precursor was dispersed into droplets and subsequently crosslinked within mineral oil (Sigma) containing 2% SPAN80 (Sigma) and a 1:15 emulsion of 30 mg/mL dithiothreitol (Sigma) on a microfluidic chip, as described previously 23 (link). Control microgels that did not contain biotin-PEG-thiol were also synthesized. After washing microgels 5 times by centrifugation in 1% bovine serum albumin (Sigma) in PBS, 104 microgels were incubated with varying concentrations of a streptavidin-AlexaFluor488 conjugate for 30 min in 500 µL PBS, and were washed 5 times by centrifugation to remove unbound SA. Microgels from each sample were placed in a 96-well plate and fluorescence was measured on a plate reader (Perkin Elmer HTS 7000). Biotin and control microgels were also synthesized with a covalently bound peptide (GRGDSPC)-AlexaFluor594 conjugate for microgel visualization, and were fluorescently imaged to confirm biotin-specific SA immobilization.
Headen D.M., Woodward K.B., Coronel M.M., Shrestha P., Weaver J.D., Zhao H., Tan M., Hunckler M.D., Bowen W.S., Johnson C.T., Shea L., Yolcu E.S., García A.J, & Shirwan H. (2018). Local immunomodulation with Fas ligand-engineered biomaterials achieves allogeneic islet graft acceptance. Nature materials, 17(8), 732-739.
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2

Biotinylated Aptamer Surface Preparation

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Biotin PEG thiol (MW 1000 Da) was purchased from Nanocs. PEG thiol (MW 550 Da) was purchased from Creative PEGWorks. Neutravidin (NA) and tris(2-carboxyethyl)phosphine hydrochloride (TCEP) were purchased from Thermo Fisher Scientific. Polystyrene beads (0.6 µm mean particle size), sulfuric acid (95.0–98.0%), hydrogen peroxide (30% w/w in H2O), and ethyl alcohol (200 proof) were purchased from Sigma-Aldrich. Paraformaldehyde (16% w/v aq soln, methanol free), Dulbecco’s Phosphate Buffered Saline (1×, without calcium and magnesium), and micro cover glass (No. 1, 18 mm × 18 mm) were purchased from VWR. Lysogeny broth and granulated agar were purchased from BD Difco. Biotinylated aptamer (Bt-aptamer, 5′[Bt]-ATACCA-GCTTATTCAATTCCCCCGTTGCTTTCGCTTTTCCTT-TCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG-AGATAGTAAGTGCAATCT3′) and fluorescently labeled biotinylated aptamer (6FAM-aptamer-Bt, 5′[6-carboxyfluorescein-ATACCAGCTTATTCAATTCCCCCGTTGCTT-TCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTG-CTTCCTTTCTTGAGATAGTAAGTGCAATCT-[Bt]3′) were synthesized by Sigma-Aldrich. Deionized (DI) water used in all experiments was prepared using a Milli-Q Gradient water purification system with a resistivity of 18.2 MΩ·cm at 25 °C (Millipore).
Hu J., Fu K, & Bohn P.W. (2018). Whole-Cell Pseudomonas aeruginosa Localized Surface Plasmon Resonance Aptasensor. Analytical chemistry, 90(3), 2326-2332.
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3

PEG-based Microgel Synthesis with Biotin

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
A microgel precursor solution containing 5% w/v PEG-4MAL (20kDa, Laysan Bio) and 1.0 mM biotin-PEG-thiol (1 kDa, Nanocs) was reacted for 15 min in PBS. This precursor was dispersed into droplets and subsequently crosslinked within mineral oil (Sigma) containing 2% SPAN80 (Sigma) and a 1:15 emulsion of 30 mg/mL dithiothreitol (Sigma) on a microfluidic chip, as described previously 23 (link). Control microgels that did not contain biotin-PEG-thiol were also synthesized. After washing microgels 5 times by centrifugation in 1% bovine serum albumin (Sigma) in PBS, 104 microgels were incubated with varying concentrations of a streptavidin-AlexaFluor488 conjugate for 30 min in 500 µL PBS, and were washed 5 times by centrifugation to remove unbound SA. Microgels from each sample were placed in a 96-well plate and fluorescence was measured on a plate reader (Perkin Elmer HTS 7000). Biotin and control microgels were also synthesized with a covalently bound peptide (GRGDSPC)-AlexaFluor594 conjugate for microgel visualization, and were fluorescently imaged to confirm biotin-specific SA immobilization.
Headen D.M., Woodward K.B., Coronel M.M., Shrestha P., Weaver J.D., Zhao H., Tan M., Hunckler M.D., Bowen W.S., Johnson C.T., Shea L., Yolcu E.S., García A.J, & Shirwan H. (2018). Local immunomodulation with Fas ligand-engineered biomaterials achieves allogeneic islet graft acceptance. Nature materials, 17(8), 732-739.
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