Cell lines, established from tumors arising in irradiated Nf1+/− F1 mice (17 (link)). Early passage stocks were established for all cell lines and experimental cells were drawn from these stocks approximately every twenty to thirty passages. Mycoplasma testing was done approximately yearly. Inhibitors were purchased from Selleck Chemicals (PD0325901 S1036, Everolimus (RAD001) S1120, Dactolisib (BEZ235) S1009, PD184352 (CI-1040) S1020, Torkinib (PP242) S2218, PIK-75 HCl S1205, Tozasertib (VX680) S1048, Barasertil (AZD1152) S1147, Alisertib (MLN8237) S1133, Binimetinib (MEK162) S7007, Trametinib (GSK1120212) S2673, Rapamycin (Sirolimus) S1039). Drug-resistant cell lines 989 PD_R, 989 RAD_R, 881 PD_R and 881 RAD_R were derived from each original parental cell line by continuous exposure to PD0325901 or RAD001. Cell lines 989 and 881 were exposed to increasing concentrations (from 25 nM to 500 nM) of PD0325901 or RAD001 for six months. Resistant cell lines were continuously maintained in medium supplemented with inhibitor. For drug washout experiments, cells were washed twice with PBS, inhibitors were withdrawn for 12 days and further analyses performed.
Pd184352 ci 1040
Manufactured by Selleck Chemicals
Sourced in China
PD184352 (CI-1040) is a small molecule that functions as a selective inhibitor of the mitogen-activated protein kinase kinase (MEK) enzyme. It is commonly used as a research tool in cell biology and biochemistry studies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pd184352 ci 1040
1
Establishing Cell Lines for Nf1 Tumorigenesis
2
Osteoclastogenesis Inhibition by 3-TYP
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3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP) (purity ≥99%) was obtained from Selleck (Shanghai, China) and dissolved in DMSO. PD184352 (CI-1040) was obtained from Selleck (Shanghai, China). Titanium (Ti) particles were purchased from Johnson Matthey Chemicals (MA, USA). Alpha modification of Eagle's medium (α-MEM), penicillin/streptomycin, and fetal bovine serum (FBS) were from HyClone (UT, USA). RANKL and M-CSF were from R&D Systems (MN, USA). Primary antibodies specific for phospho-NF-kB p65 (#3033), phospho-IkBα (#2859), NF-kB p65 (#8242), IkBα (#4814), phospho-ERK (#4370), phospho-p38 (#4511), phospho-JNK (#4668), ERK (#4695), p38 (#8690), and JNK (#9252) were from Cell Signaling Technology (MA, USA). MMP9 (ab76003) and NFATc1 (ab25916), CTSK (ab178647), c-fos (ab208942) were from Abcam (Cambridge, UK). Anti-rabbit and anti-mouse HRP-conjugated secondary antibodies were from Multi Sciences (Shanghai, China).
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