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Dopamine hydrochloride

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Dopamine hydrochloride is a chemical compound commonly used in research and laboratory settings. It is the hydrochloride salt of the neurotransmitter dopamine, which plays a critical role in the central nervous system. Dopamine hydrochloride is typically utilized as a standard or reference material in various analytical and experimental procedures.

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Lab products found in correlation

Glacial acetic acid, by Merck Group (2 mentions) D glucose, by Merck Group (2 mentions) Sodium pyruvate, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Streptomycin, by Merck Group (2 mentions) Penicillin, by Merck Group (2 mentions) Hepes, by Merck Group (2 mentions) 2 mercaptoethanol, by Merck Group (2 mentions) R deprenyl hydrochloride, by Bio-Techne (2 mentions) S sulpiride, by Bio-Techne (2 mentions) Ascorbic acid, by Bio-Techne (2 mentions) Heptanesulfonic acid, by Bio-Techne (2 mentions) Pargyline hydrochloride, by Bio-Techne (2 mentions) L dopa, by Bio-Techne (2 mentions) S raclopride tartrate salt, by Bio-Techne (2 mentions) Quinpriole hydrochloride, by Bio-Techne (2 mentions) R 2230, by Bio-Techne (2 mentions) 3h l dopa, by American Radiolabeled Chemicals (2 mentions) Quinpirole hydrochloride, by Bio-Techne (2 mentions) Histamine dihydrochloride, by Bio-Techne (2 mentions)

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Dopamine (9 citations)

8 protocols using dopamine hydrochloride

1

Dopaminergic Receptor Binding Assay

Cited 2 times
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Compounds used in this study were purchased from Sigma-Aldrich (St. Louis, MO) unless indicated otherwise: HEPES, sodium pyruvate, penicillin, streptomycin, 2-mercaptoethanol, D-glucose, bovine serum albumin (Merck Millipore, Darmstadt, Germany), glacial acetic acid, heptanesulfonic acid, methanol, glutathione, NaOH, EDTA, dimethyl sulfoxide, L-DOPA, dopamine hydrochloride, S-(−)-raclopride (+)-tartrate salt, R-(−)-deprenyl hydrochloride, pargyline hydrochloride, ascorbic acid, (S)-(−)-sulpiride (Tocris, Bristol, United Kingdom), (−)-quinpriole Hydrochloride (Tocris), R-2230 (link) (gift of Dr. Amy Newman, synthesized at NIDA, NIH, Baltimore, MD), ML32131 (link) (synthesized at NINDS, NIH, Bethesda, MD) and [3H]L-DOPA (Dihydroxyphenylalanine, L-3,4-[ring 2,5,6-3H]) (American Radiolabeled Chemicals, St. Louis, MO).
Farino Z.J., Morgenstern T.J., Maffei A., Quick M., De Solis A.J., Wiriyasermkul P., Freyberg R.J., Aslanoglou D., Sorisio D., Inbar B.P., Free R.B., Donthamsetti P., Mosharov E.V., Kellendonk C., Schwartz G.J., Sibley D.R., Schmauss C., Zeltser L.M., Moore H., Harris P.E., Javitch J.A, & Freyberg Z. (2019). New roles for dopamine D2 and D3 receptors in pancreatic beta cell insulin secretion. Molecular psychiatry, 25(9), 2070-2085.
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2

Dopaminergic Signaling Modulation Assay

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CNO (Tocris) was first diluted in DMSO (Sigma), then in Dulbecco’s Phosphate Buffered Saline (PBS, Gibco) to a 0.5% DMSO final concentration and injected intraperitoneally at 3 mg/kg 15 min before behavioral testing. Dopamine hydrochloride (Tocris, #3548), SKF81297 hydrobromide (Tocris, #1447) and (−)-Quinpirole hydrochloride (Tocris, #1061) were diluted in ACSF (see below) to respectively 10, 50 and 10 µM, extemporaneously for each day of recording, and kept protected from light for the duration of the bath perfusion.
Godino A., Salery M., Minier-Toribio A.M., Patel V., Fullard J.F., Parise E.M., Martinez-Rivera F.J., Morel C., Roussos P., Blitzer R.D, & Nestler E.J. (2023). Dopaminoceptive D1 and D2 neurons in ventral hippocampus arbitrate approach and avoidance in anxiety. bioRxiv.
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3

Immortalized Rat Retinal Endothelial Cell Response to Dopamine and Cytokines

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The immortalized rat retinal capillary endothelial cells SV40 (T9097) and the corresponding applied cell extracellular matrix (G422) and Prigrow IV medium (TM004) supplemented with 10% fetal bovine serum (FBS) were obtained from Applied Biological Materials (Richmond, BC, Canada). The cells were seeded into 24-well plates at 50,000 cells/well and pretreated with dopamine hydrochloride (25, 50, and 100 μM; Tocris Bioscience, Bristol, UK) for 2 h followed by a 24 h treatment with or without cytokine cocktail containing IL-6 (100 pg/μL; Thermo Fisher Scientific, Waltham, MA, USA), sIL-6R (100 pg/μL; Thermo Fisher Scientific, Waltham, MA, USA), and IL-17A (50 pg/μL; Thermo Fisher Scientific, Waltham, MA, USA). The cells were then processed for RNA isolation and qRT-PCR or cytoplasmic and nuclear protein extractions using Minute™ Cytoplasmic and Nuclear Extraction Kit for Cells (Invent Biotechnologies, Plymouth, MN, USA) for Western blotting.
Stofkova A., Zloh M., Andreanska D., Fiserova I., Kubovciak J., Hejda J., Kutilek P, & Murakami M. (2021). Depletion of Retinal Dopaminergic Activity in a Mouse Model of Rod Dysfunction Exacerbates Experimental Autoimmune Uveoretinitis: A Role for the Gateway Reflex. International Journal of Molecular Sciences, 23(1), 453.
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4

Neuroactive Compounds Acquisition Protocol

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Serotonin hydrochloride, Histamine dihydrochloride, Asenapine maleate were purchased from Tocris, Dopamine hydrochloride, Epinephrine hydrochloride, [Arg8]-Vasopressin acetate salt, Paliperidone, Somatostatin were obtained from Sigma-Aldrich, Aripiprazole was purchased from Toronto Research Chemical.
Galinski S., Wichert S.P., Rossner M.J, & Wehr M.C. (2018). Multiplexed profiling of GPCR activities by combining split TEV assays and EXT-based barcoded readouts. Scientific Reports, 8, 8137.
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5

Neurochemical Receptor Assay Protocols

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Ethanol was purchased from Pharmco-AAPER (Brookfield, CT). Dopamine hydrochloride, sulpiride, SCH23390, nomifensine, N-[3-([1,1-Biphenyl]-4-yloxy)-3-(4-fluorophenyl)propyl]-N-methylglycine (NFPS), nisoxetine hydrochloride bicuculline, chelerythrine and Gö6976 were purchased from Tocris Biosciences (Minneapolis, MN). The cDNA plasmid pZac2.1-GfaABC1D-hPMCA2w/b-mCherry was purchased from Addgene (plasmid #111568) and used to generate an AAV2/5 virus (Univ. of South Carolina Viral Core, Columbia, SC). The AAV5-GfaABC1D-tdTomato virus was purchased from Addgene. U73122 was purchased from Abcam (Cambridge, MA). Strychnine, VU0134992 hydrochloride, sulforhodamine 101 and all reagents used to prepare aCSF, sucrose-containing and internal pipette solution were purchased from Sigma (St. Louis, MO).
Nimitvilai-Roberts S., Gioia D., Zamudio P.A, & Woodward J.J. (2021). Ethanol inhibition of lateral orbitofrontal cortex neuron excitability is mediated via dopamine D1/D5 receptor-induced release of astrocytic glycine. Neuropharmacology, 192, 108600.
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6

Dopaminergic Receptor Ligand Assays

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Terguride (ab144611) was obtained from Abcam. Aripiprazole (SML-0935), PMA (P8139) and pergolide mesylate (P8828) were purchased from Sigma-Aldrich. Apomorphine hydrochloride (2073), MLS1547 (6171), ropinirole (3680), quinpirole hydrochloride (1061), dopamine hydrochloride (3548), cabergoline (2664), bromocriptine mesylate (0427), forskolin (1099), SCH23390 (0925), PTX (3097), haloperidol hydrochloride (0931), L-741,626 (1003) and roxindole (1559) were obtained from Tocris. UNC9994 (A16087) was purchased from AdooQ Bioscience. Lambda-phosphatase (P0753S) was obtained from Santa Cruz. Compound 101 (HB2840) was obtained from Hello Bio. Terguride, PMA, forskolin, L-741,626, aripiprazole, pergolide, apomorphine, MLS1547, ropinirole, cabergoline, bromocriptine, haloperidol, roxindole, UNC9994 and compound 101 are DMSO-soluble and all the other mentioned compounds are water-soluble.
Mann A., Keen A.C., Mark H., Dasgupta P., Javitch J.A., Canals M., Schulz S, & Robert Lane J. (2021). New phosphosite-specific antibodies to unravel the role of GRK phosphorylation in dopamine D2 receptor regulation and signaling. Scientific Reports, 11, 8288.
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7

Dopaminergic Receptor Binding Assay

Cited 2 times
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Compounds used in this study were purchased from Sigma-Aldrich (St. Louis, MO) unless indicated otherwise: HEPES, sodium pyruvate, penicillin, streptomycin, 2-mercaptoethanol, D-glucose, bovine serum albumin (Merck Millipore, Darmstadt, Germany), glacial acetic acid, heptanesulfonic acid, methanol, glutathione, NaOH, EDTA, dimethyl sulfoxide, L-DOPA, dopamine hydrochloride, S-(−)-raclopride (+)-tartrate salt, R-(−)-deprenyl hydrochloride, pargyline hydrochloride, ascorbic acid, (S)-(−)-sulpiride (Tocris, Bristol, United Kingdom), (−)-quinpriole Hydrochloride (Tocris), R-2230 (link) (gift of Dr. Amy Newman, synthesized at NIDA, NIH, Baltimore, MD), ML32131 (link) (synthesized at NINDS, NIH, Bethesda, MD) and [3H]L-DOPA (Dihydroxyphenylalanine, L-3,4-[ring 2,5,6-3H]) (American Radiolabeled Chemicals, St. Louis, MO).
Farino Z.J., Morgenstern T.J., Maffei A., Quick M., De Solis A.J., Wiriyasermkul P., Freyberg R.J., Aslanoglou D., Sorisio D., Inbar B.P., Free R.B., Donthamsetti P., Mosharov E.V., Kellendonk C., Schwartz G.J., Sibley D.R., Schmauss C., Zeltser L.M., Moore H., Harris P.E., Javitch J.A, & Freyberg Z. (2019). New roles for dopamine D2 and D3 receptors in pancreatic beta cell insulin secretion. Molecular psychiatry, 25(9), 2070-2085.
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8

Cocktail Stimulation of Neurotransmitters

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A cocktail was prepared freshly with AMPA (1 μM, Sigma-Aldrich), noradrenaline (1 μM, Sigma-Aldrich), kainic acid (kainate), ibotenic acid, serotonin, histamine dihydrochloride, dopamine hydrochloride, and N-methyl-D-aspartate (NMDA) (each 1 μM, from Tocris Bioscience), carbachol (10 μM, Sigma-Aldrich), and orexin (0.01 μM, Sigma-Aldrich). Distilled H 2 O was added in sister cultures as control in all stimulation protocols. To avoid differences in gene expression caused circadian rhythms, stimulations and RNA and protein extractions from different conditions were performed at the same circadian time. RNA was extracted three hours after stimulation and again 24 hours later (recovery).
Mikhail C., Vaucher A., Jimenez S, & Tafti M. (2017). ERK signaling pathway regulates sleep duration through activity-induced gene expression during wakefulness. Science signaling, 10(463).
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