Joel jfc 1600 auto fine coater

The shape and size of the nanoparticles of MTCA-KKV were visualized on scanning electron microscopy (SEM, S-4800, Hitachi, Tokyo, Japan) at 50 kV. For this purpose, the solution (10 μM, 0.1 μM and 1 nM) of MTCA-KKV in the ultrapure water of pH 6.8 was lyophilized and the powders were attached to the copper plate with double-sided tape (Euromedex, Strasbourg, France). Using Joel JFC-1600 Auto Fine Coater (JEOL, Japan) at 15 kV, 30 mA and 200 mTorr (argon) for 60 s the specimens were coated with 20 nm gold-palladium. The feature and size of the nanoparticles were identified by examining more than 100 species in randomly selected region on the SEM alloy. For each sample, triplicate grids were prepared and examined. To record 100–10,000× digitally enlarged SEM images, an imaging plate (Gatan Bioscan Camera Model 1792, Pleasanton, CA, USA) was used, and the energy window was 20 eV.

The morphology and size of the lyophilized powders of IQCA-TAVV were imaged on an SEM (S-4800; Hitachi, Tokyo, Japan) at 15 kV. The SEM sample was prepared by fixing the lyophilized powders from a 10⁻⁸ M solution of IQCA-TAVV in ultrapure water onto the stub with double-sided tape (Euromedex, Strasbourg, France), which was then coated with 10-nm-thick gold–palladium by use of a Joel JFC-1600 auto fine coater (JEOL, Tokyo, Japan). The imaging conditions were 15 kV, 30 mA and 200 mTorr (argon) for 60 s. The randomly selected regions of the coater were viewed, and the morphology and size distribution of the lyophilized powders were recorded for over 100 species. Then 100–10,000× digitally enlarged lyophilized powders were recorded on the imaging plate (Gatan Bioscan Camera Model 1792) with 20 eV energy windows. Each imaging was performed with triplicate samples.