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Il10 human elisa kit

Manufactured by Thermo Fisher Scientific
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The IL10 Human ELISA kit is a quantitative enzyme-linked immunosorbent assay (ELISA) used for the measurement of interleukin-10 (IL-10) levels in human serum, plasma, and cell culture supernatants. It is designed to provide accurate and reliable quantification of IL-10 in a simple and efficient manner.

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Lab products found in correlation

96 well cell culture plate, by Corning (1 mentions) Ifn gamma human elisa kit, by Thermo Fisher Scientific (1 mentions)

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ELISAs (35 citations)

2 protocols using il10 human elisa kit

1

Quantifying IL2-CD25 Complexes and IL10 Secretion

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To determine whether the fractional occupancy of IL2-CD25 complexes correlated with IL10 secretion, primary human CD4+ T cells were cultured with IL2-CD25 complex-coated Dynabeads created at different fractional occupancies (89% to 0%) using His-CD25-coated Dynabeads (as above) and 0 nM to 160 nM of IL2. Cells were cultured in a 96-well cell culture plate (Corning) for 3 days at 37°C. Fifty μL of cell culture supernatant per well was collected, and IL10 was immediately measured using the IL10 Human ELISA kit following the manufacturer-recommended protocol (Thermo Fisher).
Veeramani S., Blackwell S.E., Thiel W.H., Yang Z.Z., Ansell S.M., Giangrande P.H, & Weiner G.J. (2019). An RNA aptamer-based biomarker platform demonstrates high soluble CD25 occupancy by IL2 in the serum of follicular lymphoma patients. Cancer immunology research, 7(9), 1511-1522.
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2

Quantifying IFN-γ and IL-10 in Plasma

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Plasma levels of IFN-γ and IL-10 were quantified using an enzyme immunoassay (ELISA), IFN gamma Human ELISA Kit, and IL-10 Human ELISA Kit (Thermo Fisher, Carlsbad, CA, USA), which use specific monoclonal antibodies to detect each of the cytokines. The test was performed according to the manufacturer’s instructions.
Souza I.D., Amoras E.D., de Sousa F.D., de Sousa P.V., Lima S.S., Cayres-Vallinoto I.M., Ishak R., Vallinoto A.C, & Queiroz M.A. (2022). Plasma Levels of sFas-sFasL and FASL Gene Expression Are Associated with Tuberculosis. Biomolecules, 13(1), 80.
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