Anti tcrγδ pe

Manufactured by BD

Sourced in United States, Spain

Anti-TCRγδ-PE is a fluorochrome-conjugated antibody that binds to the T-cell receptor (TCR) gamma-delta (γδ) complex on the surface of T cells. This antibody is used in flow cytometry applications to identify and quantify γδ T cells within a cell population.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using anti tcrγδ pe

Multiparametric Flow Cytometry for Innate Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

EDTA-treated whole blood was incubated using the corresponding monoclonal antibodies: anti-CD3-PC5.5 and anti-CD4-APC-A750 (all from Beckman Coulter, Miami, FL, USA); anti-TCRγδ-PE, anti-NKG2D-PCy7 and anti-CD8-APC (all from BDBiosciences, Franklin Lakes, NJ, USA); and anti-CD56-FITC and anti-CD161-FITC (all from BioLegend, San Diego, CA, USA). Innate subsets were analyzed by flow cytometry using a Dx-Flex Cytometer and Kaluza Software (Beckman Coulter, Miami, FL, USA).
NK and NKT cells were considered CD3-CD56+ and CD3 + CD56+, respectively, gated from lymphocytes. MAIT cells were considered Vα7.2+ and CD161+ gated from CD3 + αβTCR+ T cells. MAIT cells expressing CD4 and CD8 were gated from CD3 + CD4+ or CD8+ lymphocytes, respectively, expressing both Vα7.2 and CD161.

Garcinuño S., Gil-Etayo F.J., Mancebo E., López-Nevado M., Lalueza A., Díaz-Simón R., Pleguezuelo D.E., Serrano M., Cabrera-Marante O., Allende L.M., Paz-Artal E, & Serrano A. (2022). Effective Natural Killer Cell Degranulation Is an Essential Key in COVID-19 Evolution. International Journal of Molecular Sciences, 23(12), 6577.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proportions of DN T cells were determined in total blood samples by flow cytometry using a Navios Cytometer (Beckman Coulter, Madrid, Spain). Mouse anti-human monoclonal antibodies were used to identify this population: anti-TCRαβ-FITC, anti-TCRγδ-PE, anti-CD3-PerCP5.5 (BD Biosciences, Madrid, Spain), anti-CD8-PeCy7, anti-CD4-APC, and anti-CD45-APC-Alexa Fluor 750 (Beckman Coulter). Activated T-cells and B-cell phenotype were also tested in the ALPS-FASLG patient. Mouse anti-human monoclonal antibodies were used to identify these populations: anti-HLA-DR-PE, anti-CD3-PerCP5.5, anti-IgD-PE (BD Biosciences), anti-CD19-FITC, and anti-CD27-PC5 (Beckman Coulter).
Plasma levels of IL-10 (Bender MedSystems, LabClinics, Madrid, Spain), sCD25 and sFasL (R&D Systems, Vitro, Madrid, Spain) and vitamin B12 (Beckman Coulter) were measured in duplicate by enzyme-linked immunosorbent assay. Serum immunoglobulin levels (IgG, IgA, IgM) were measured by nephelometry (Beckman Coulter).

Ruiz-García R., Mora S., Lozano-Sánchez G., Martínez-Lostao L., Paz-Artal E., Ruiz-Contreras J., Anel A., González-Granado L.I., Moreno-Pérez D, & Allende L.M. (2015). Decreased activation-induced cell death by EBV-transformed B-cells from a patient with autoimmune lymphoproliferative syndrome caused by a novel FASLG mutation. Pediatric research, 78(6).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!