Chromatin immunoprecipitation tests were performed using the Millipore ChIP kit (17-371RF) following the manufacturer’s instructions. The 10 μg ZBTB7A antibody (Santa Cruz Biotechnology, SC-33683X) was used. The following specific primers HIC1 promoter F: 5-GGATCTGGGCGCACCTCA-3 and R: 5- GTCTACGGGGAGTTCC-3 were used for RT-PCR for binding DNA fragments.
Zbtb7a antibody
Manufactured by Santa Cruz Biotechnology
The ZBTB7A antibody is a research-use only tool for the detection and study of the ZBTB7A protein. ZBTB7A is a transcription factor that plays a role in cell differentiation and development. The antibody can be used to identify and analyze the ZBTB7A protein in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Chip kit, by Merck Group (1 mentions)
Rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions)
Caspase 3 antibody, by Cell Signaling Technology (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Antibody against gapdh, by Santa Cruz Biotechnology (1 mentions)
C ebpβ antibody, by Santa Cruz Biotechnology (1 mentions)
Il 24, by Proteintech (1 mentions)
3 protocols using zbtb7a antibody
1
Chromatin Immunoprecipitation of ZBTB7A
2
ZBTB7A Knockdown in Huh7 Cells
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To knockdown ZBTB7A, we utilized siRNA targeting ZBTB7A (Thermo, siRNA ID 147596, AM16708) and negative control siRNA (Thermo, AM4611). Huh7 cells were transfected with 50nM siRNA using RNAiMAX Transfection Reagent (Thermo, 13778150) and infected with HCoV-229E as indicated. ZBTB7A mRNA and protein levels were detected with the following probe (Thermo, Hs00792219_m1) and ZBTB7A antibody (Santa Cruz, sc-33683).
3
Osteosarcoma Cell Lines and Antibody Analysis
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The human osteosarcoma cancer cell lines U2OS and MG63 were obtained from the American Type Culture Collection. U2OS cells were cultured in DMEM with 10% fetal bovine serum (FBS; ExCell Bio, Lot: FSP500). MG63 cells were cultured in EMEM medium with 10% fetal bovine serum (FBS; ExCell Bio, Lot: FSP500). The medium was renewed every day, and cells were passaged before reaching confluence. The following antibodies were used in this study: antibody against GAPDH (Santa Cruz Biotechnology, Dallas, TX; SC-25778); caspase 3 antibody (Cell Signaling Tech, 9662); PARP (Santa Cruz Biotechnology, SC-8007); ZBTB7A antibody (Santa Cruz Biotechnology, SC-33683); C/EBPβ antibody (Santa Cruz Biotechnology, SC-150); C/EBPα antibody (Santa Cruz Biotechnology, SC-9351); IL24 (Proteintech, 26,772–1-AP); and cisplatin (Sigma, P4394).
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