Anti h4k16ac

qChIP was performed as described33 (link) with the following modifications: 5 ml of an overnight culture grown in YPAD with extra uridine (0.08 mg/ml), diluted into fresh YPAD with extra uridine (0.08 mg/ml) and grown until OD600 nm of 1.4. Cells (50 ml/sample) were fixed with 1% Paraformaldehyde (Sigma) for 15 min at room temperature. Cells were lysed using acid-washed glass beads (Sigma) and a Disruptor genie^TM (Scientific Industries) for 30 min at 4 °C. Chromatin was sheared to 500–1000 bp using a Bioruptor (Diagenode) for a total of 20 min (30 s ON and OFF cycle) at 4 °C. Immunoprecipitation was performed overnight at 4 °C using 2 μL of antibody anti-H3K4me2 (Active Motif- Cat Number: 39141), anti-H3K9ac (Active Motif- Cat Number: 39137), and anti-H4K16ac (Active Motif- Cat Number: 39167) and 25 μl of Protein G magnetic beads (Dynal - InVitrogen). DNA was eluted with a 10% slurry of Chelex 100-resin (Bio-Rad) using the manufacturer’s instructions.