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5 protocols using tetrabutylammonium hydroxide tba oh

1

Biomimetic Hydrogel for Cell Culture

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All chemicals were used as received unless stated otherwise. Sodium hyaluronate (64 kDa) was purchased from Lifecore Biomedical (Chaska, MN). Dowex™ 500WX8–200 ion-exchange resin (AC335341000) was purchased from Fisher Scientific. GelMA (76292–720) and Seakem® Agarose (12002–102) were purchased from VWR. Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) was purchased from Colorado Photopolymer Solutions (Boulder, CO). Alexa Fluor™ 647 Phalloidin (A22287), LIVE/DEAD™ Viability/Cytotoxicity kit (L3224), and Hoechst 33342 (H3570) were purchased from Thermo Fisher Scientific. Tetrabutylammonium hydroxide (TBA-OH, 86880–100ML), anhydrous dimethyl sulfoxide (276855), 5-norbornene-2-carboxylic acid (446440), 4-(dimethylamino)pyridine (107700), di-tert-butyl dicarbonate (361941), 4-(dimethylamino)pyridine (107700), tetrabutylammonium hydroxide solution (86880), DL-dithiothreitol (D0632), poly(ethylene oxide) (189456), 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (I2959) (410896), fluorescein isothiocyanate-dextran (average molecular weight 2 MDa, FD2000S-1G) and 3-(trimethoxysilyl)propyl methacrylate (TMSPMA) (440159–500ML) were purchased from Sigma-Aldrich (St. Louis, MO).
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2

Electrochemical Characterization of Ge/GeO2

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Potassium carbonate (K2CO3), ruthenium dioxide (RuO2), and tetrabutylammonium hydroxide (TBA+OH) solutions (~40 wt% in water) were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA). Hydrochloric acid (HCl, 35–37%) was acquired from Duksan Pure Chemical Co., Ltd. (Ansan, Korea). Ethylamine (70% in water) was obtained from Junsei Chemical Co., Ltd. (Tokyo, Japan). Ge/GeO2 powder (99.9% purity, 35 nm) was purchased from RNDKOREA (Gwangmyeong, Korea).
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3

Biomaterial Fabrication for BMP-2 Delivery

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Sodium hyaluronate (HA, 40 kDa and 100 kDa) was purchased from Lifecore Biomedical LLC (Chaska, MN). Adipic acid dihydrazide (ADH) was purchased from Spectrum Chemical (Gardena, CA). Hydroxybenzotriazole (HOBt) was purchased from Chem Impex (Wood Dale, IL). 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was purchased from G Biosciences (St. Louis, MO). Sodium Periodate, AmberLite® MB Ion Exchange Resin, tetrabutylammonium hydroxide (TBA-OH), DMSO, 4-dimethylaminopyridine (DMAP), di-tert-butyl decarbonate (BoC2O) and 5-norbornene-2-carboxylic acid were purchased from Sigma Aldrich (St. Louis, MO). Irgacure 2959 was purchased from Advanced Biomatrix (Carlsbad, CA). Recombinant human bone morphogenetic protein-2 (BMP-2) and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA) were purchased from R&D Systems (Minneapolis, MN).
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4

Hydrofluoric and Hydrochloric Acid Protocol

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Hydrofluoric acid HF (48
wt %, 27.58 M), hydrochloric acid HCl (37 wt %, 12.17 M), and tetrabutylammonium
hydroxide TBAOH (40 wt % in water) were acquired from Sigma-Aldrich,
Germany.
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5

Preparation of Organic Compounds

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Dry solvents were obtained from VWR. 4,4′-Azobis(4cyanovaleric acid), sebacic acid, 4-penten-1-ol, 3,3′thiodipropanoic acid, 5-hexen-1-ol, 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimidehydrochloride (EDCI), 4dimetylaminopyridine (DMAP), second generation Grubbs catalyst (Grubbs II catalyst), butylated hydroxytoluene (BHT), and tetrabutyl ammonium hydroxide (TBAOH) were purchased from Sigma-Aldrich. 7-Octene-1-ol was purchased from TCI. 4-((4-Carboxybenzyl)oxy)benzoic acid, (19) (link) gDCCcyclooctadiene, (20) (link) and Grubbs-py (21) (link) were prepared according to procedures published previously.
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