HPLC-grade acetonitrile and methanol were from Merck. Hydrochloric acid (HCl), glycine and hydroxylamine (>97.5%) were purchased from Reakhim. DEEMM and L-ornithine monohydrochloride (≥99%) was obtained from Fluka. Boric acid (≥99%) was purchased from Hopkin & Williams. Amino acid commercial standards (L-alanine, L-arginine hydrochloride, L-asparagine, L-aspartic acid, L-glutamine, L-glutamic acid, L-glycine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine and L-valine, ≥99.5%), γ-aminobutyric acid (≥99%), formic acid (≥95%), putrescine (≥99.5%), phenyethylamine (≥99.5%), tyramine hydrochloride (≥98%), ethylamine hydrochloride (98%), methylamine solution (40%), pyrrolidine (99%), ethanolamine (≥98%) and taurine (≥99%) were purchased from Sigma; dopamine hydrochloride (99%), histamine dihydrochloride (≥98%) were purchased from Alfa Aesar; L(+)-α-aminobutyric acid (98%) were purchased from Acros Organics; β-aminobutyric acid (98%) were purchased from BLD Pharmatec. Ultrapure water was supplied by a Millipore Milli-Q Advantage A10 (Millipore) and 0.75 M borate buffer at pH = 9 was prepared in deionized water.
L arginine hydrochloride
Manufactured by Merck Group
Sourced in United States, Germany
L-arginine hydrochloride is a chemical compound that serves as a key raw material in various laboratory applications. It is a salt of the amino acid L-arginine and hydrochloric acid. The compound is commonly used in biochemical research, pharmaceutical development, and analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
L lysine hydrochloride, by Merck Group (7 mentions)
L asparagine, by Merck Group (4 mentions)
L glutamic acid, by Merck Group (4 mentions)
L aspartic acid, by Merck Group (4 mentions)
L tryptophan, by Merck Group (4 mentions)
γ aminobutyric acid, by Merck Group (3 mentions)
L alanine, by Merck Group (3 mentions)
Cerulein, by Merck Group (3 mentions)
L phenylalanine, by Merck Group (3 mentions)
L methionine, by Merck Group (3 mentions)
L threonine, by Merck Group (3 mentions)
L valine, by Merck Group (3 mentions)
L isoleucine, by Merck Group (3 mentions)
L serine, by Merck Group (3 mentions)
L tyrosine, by Merck Group (3 mentions)
L proline, by Merck Group (3 mentions)
L glutamine, by Merck Group (3 mentions)
L leucine, by Merck Group (3 mentions)
Dspe peg, by NOF corporation (3 mentions)
Tyramine hydrochloride, by Merck Group (2 mentions)
24 protocols using l arginine hydrochloride
1
HPLC Analysis of Amino Acids and Amines
2
Inflammatory Pain Modulation Protocols
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L-arginine hydrochloride (NO synthase (NOS) substrate), L-NAME (non-selective inhibitor of NO synthase), and diazoxide (an ATPsensitive K + channel opener) were obtained from Sigma-Aldrich (St. Louis, Missouri, USA). Methylene blue (NOS and guanylyl cyclase inhibitor) was provided by Merck. Glibenclamide (ATP-sensitive K + channel inhibitor) and carbamazepine were kindly donated by Darupakhsh and Abidi Pharmaceutical Co. (Tehran, Iran), respectively. Pioglitazone (a PPARγ agonist) and GW-9662 (a PPARγ antagonist) were purchased from Osveh Pharmaceutical Co, and Tocris Bioscience, respectively. All drugs were dissolved or suspended in normal saline (0.9 % NaCl) and buffered to a pH of 7.3. Drug concentrations were freshly prepared in such a way that the necessary dose could be injected in a volume of 50 µL/paw by the intraplantar (i.pl.) route. Doses and drug administration schedules (▶Fig. 1) were selected based on previous reports and also our experience in the laboratory [7, 11, 17, 20] .
3
Investigating HSF1 and SIRT1 Regulation
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The antibodies to HSF1 (1:2,000 dilution; 12972S), acetylated-lysine (1:1,000 dilution; 9441S), SIRT1 (1:2,000 dilution; 9475S) and p53 (1:1,000 dilution; 2524S) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The antibody to glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:3,000 dilution; G9545), resveratrol, L-arginine hydrochloride, sodium carboxymethyl cellulose, protease inhibitor cocktail were purchased from Sigma-Adrich (St. Louis, MO, USA). Mouse TNF-α, IL-6 and IL-10 enzyme-linked immunosorbent assay (ELISA) kits and IgG horseradish peroxidase-conjugated secondary antibody (1:10,000 dilution; BA1050, BA1054) were purchased from Boster Biological Technology, Ltd. (Wuhan, China). Lactate dehydrogenase and myeloperoxidase (MPO) assay kit were purchased from Nanjing Jiancheng Biology Engineering Institute. In situ cell death detection kit was purchased from Roche Applied Sciences (Mannheim, Germany). PureProteome™ Protein A and Protein G magnetic beads were purchased from Merck Millipore (Billerica, MA, USA). Other reagents were analytically pure.
4
Pharmacological Modulators of Vascular Function
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Nicotine (Merck Schuchardt OHG, Hohenbrunn, Germany), acetylcholine chloride, hemin, Nw-nitro-L-arginine methylester hydrochloride (L-NAME), L-arginine hydrochloride, papaverine hydrochloride, mifepristone, phenylephrine hydrochloride, N-ethylcarboxamidoadenosine, 17β-estradiol, zinc protoporphyrin IX (ZnPP, Sigma Chemical Co., St. Louis, MO, U.S.A.), medroxyprogesterone acetate (Pfizer, New York, U.S.A.), thiopental sodium (Thiopental, Biochemie GmbH, Vienna, Austria) were purchased from commercial vendors.
5
Pharmacological Modulation of Nitric Oxide Pathway
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L-arginine hydrochloride (NO synthase substrate), L-NAME (non-selective inhibitor of NO synthase), and diazoxide (an ATP-sensitive K channel opener) were obtained from Sigma-Aldrich (St. Louis, Missouri, United States). Montelukast, sildenafil, and glibenclamide (ATP-sensitive K channel inhibitor) were kindly donated by Sobhan Pharmaceutical Co. (Tehran, Iran). Methylene blue (MB; guanylate cyclase inhibitor) was obtained from Merck. The used drugs were dissolved or suspended in normal saline (0.9% NaCl) and injected in a volume of 10 ml/kg. The selection of doses and route of administration were based on previous studies and also experiments in our laboratory (Mansouri et al., 2018 (link); Naserzadeh et al., 2019 (link); Dejban et al., 2020 (link); Ajaman et al., 2021 (link)).
6
Induction and Assessment of Severe Acute Pancreatitis
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SAP was induced by two intraperitoneal injections of L-arginine hydrochloride (Sigma-Aldrich, St. Louis, MO, USA) (8%, pH=7.0), 4 g/kg bodyweight each, with 1-hour interval between injections as previously reported.15 (link) Sham injections of NaCl were administrated to normal controls. Mice were then injected with phosphate-buffered saline (PBS) or recombinant murine IL-22 (rIL-22; 200 ng/mouse, Miltenyi Biotec, Bergisch Gladbach, Germany) at specified times. All mice were returned to the cages, with free access to food and water. SAP group mice were sacrificed at 24, 48, and 72 hours after the second injection of L-arginine, respectively.
7
Comprehensive Cell Culture Medium Formulation
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Glycine, L-alanine, L-arginine hydrochloride, L-asparagine, L-aspartic acid (Sigma), L-cysteine, L-cystine hydrochloride, γ-amino butyric acid, L-glutamic acid, L-glutamine, L-histidine, L-methionine, L-isoleucine, L-leucine, L-proline, L-lysine hydrochloride, L-phenylalanine, L-serine, L-threonine, L-tryptophane, L-tyrosine, biotin, calcium pantothenate, choline chloride, folic acid, inositol, nicotinic acid, riboflavin, thiamine hydrochloride, ascorbic acid (Sigma, Kanagawa, Japan), pyridoxine hydrochloride (Aladdin), CaCl2·2H2O (Macklin), CuCl2·2H2O (Macklin), FeCl3·6H2O (Aladdin), L-valine (Solarbio), MnCl2·4H2O (Macklin), ZnCl2 (Aladdin), MgCl2·6H2O (Aladdin), KH2PO4 (Aladdin), sucrose (Aladdin).
8
SILAC Cell Culture Labeling Protocol
9
Mouse Models of Pancreatitis Induction
10
Amino Acid Supplementation Protocol
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l -Arginine hydrochloride, l -lysine hydrochloride, l -ornithine hydrochloride, l -ornithine aspartate, l -glucose and primers were purchased from Sigma Aldrich. Difluoromethyl ornithine (DFMO) was kindly supplied by Dr Patrick M Woster (Wayne State University).
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