Concentrations of lactate, acetate, phosphate, succinate, nitrate, nitrite, chloride and sulphate were determined in pot ale by Ion Chromatography (IC) using a Dionex Integrion HPIC system (Thermo Fisher Scientific, UK). Analytes were separated on a Dionex IonPac AS11-HC-4 µm column (4 × 250 mm) equipped with a Dionex IonPac AG11-HC-4 µm guard column (4 × 50 mm) and Dionex AERS500 suppressor. A diluted KOH / ultrapure water mobile phase was used with a gradient elution program of the following: 0–8 min (1.5 mM), 8–18 min (15 mM), 18–23 min (15 mM), 23–24 min (24 mM) and 24–30 min (60 mM). A flow rate of 0.38 mL min–1 was used. A sample volume of 2 µL was injected once the background conductivity was < 1 µS. Data acquisition and analysis was performed using Chromeleon 7.1 software. Calibration curves were generated using analytical standards (Sigma-Aldrich, UK), at a range of 5–100 mg/L. Pot ale samples were diluted 1/100 prior to analysis. Pot ale samples (triplicate) spiked at a concentration of 10 mg L−1 using analytical standards of acetate, lactate, chloride, nitrite, succinate, carbonate, sulphate, phosphate and nitrate (Sigma-Aldrich, UK) gave recoveries ranging between 95–103%.
Aers 500 suppressor
Manufactured by Thermo Fisher Scientific
The AERS 500 suppressor is a lab equipment product designed to reduce the sound pressure level of various instruments and equipment in a laboratory setting. It functions by absorbing and dissipating acoustic energy, thereby lowering the noise output of the target device.
Automatically generated - may contain errors
Lab products found in correlation
Dionex integrion hpic system, by Thermo Fisher Scientific (1 mentions)
Analytical standards, by Merck Group (1 mentions)
Ion chromatography system, by Thermo Fisher Scientific (1 mentions)
Ionpac as19, by Thermo Fisher Scientific (1 mentions)
Ionpac cg12a guard column, by Thermo Fisher Scientific (1 mentions)
Ionpac cs12a analytical column, by Thermo Fisher Scientific (1 mentions)
3 protocols using aers 500 suppressor
1
Ion Chromatographic Analysis of Pot Ale
2
Adsorption of 2,4-D from Aqueous Solution
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The stock solution of 2,4-D at 1000 mg L -1 were prepared by dissolving solid standards in methanol. Daily used solutions were made from successive dilution of the stock solution with ultrapure water to appropriate concentrations. The concentrations of 2,4-D were analyzed by Ion Chromatography system (ThermoFisher Scientific Aquion) equipped with a Dionex AERS 500 suppressor (AERS 4 mm) and an EGC eluent generator. An analytical column Dionex IonPacAS19 (4 × 250 mm) was used at 30 °C. The mobile phase was 20 mM NaOH aqueous solution produced by eluent generator.
The batch adsorption experiment was conducted using a series of 250 mL conical flasks with a certain account of adsorbents and 20 mL of 2,4-D solution, and the flasks were placed in a constant temperature shaker for a fixed time. After adsorption, the adsorbents were separated by centrifuge from aqueous phase, and the solution was filtered with a 0.22 μm nylon membrane. Then, remaining concentration of 2,4-D in filtrate was measured by ion chromatography. The adsorption capacity q e (mg g -1 ) was expressed by Eq. ( 1):
where C 0 and C e (mg L -1 ) mean the liquid-phase concentrations of 2,4-Dat initial and equilibrium, respectively. W(g) is the mass of the adsorbent, and V(L) is the volume of solution.
The batch adsorption experiment was conducted using a series of 250 mL conical flasks with a certain account of adsorbents and 20 mL of 2,4-D solution, and the flasks were placed in a constant temperature shaker for a fixed time. After adsorption, the adsorbents were separated by centrifuge from aqueous phase, and the solution was filtered with a 0.22 μm nylon membrane. Then, remaining concentration of 2,4-D in filtrate was measured by ion chromatography. The adsorption capacity q e (mg g -1 ) was expressed by Eq. ( 1):
where C 0 and C e (mg L -1 ) mean the liquid-phase concentrations of 2,4-Dat initial and equilibrium, respectively. W(g) is the mass of the adsorbent, and V(L) is the volume of solution.
3
Soil Ion Content Analysis by IC
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The ion content of the soil solutions was analyzed using the ion chromatography technique (IC) and a Dionex DX600 model IC. The different ions were separated with two different analytical columns: anions (Cl -, SO 4 2-, NO 3 -) were separated with a Dionex IonPac CS12A analytical column, Dionex IonPac CG12A guard column, Dionex DRS 600 suppressor and H 2 SO 4 25mN eluent with a 1 mL/min flux; for cations (Na + , K + , Ca 2+, Mg 2+ , NH 4 + ), separation was carried out with a Dionex IonPac AS9-HC analytical column, Dionex IonPac AGS9-HC guard column, Dionex AERS 500 suppressor and Na 2 CO 3 9 mM eluent with a 1 mL/ min flux. NO 3 -and NH 4 + concentrations were undetectable in most samples.
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