Melting points were recorded by X-5 micromelting-point apparatus. Optical rotations were measured on a Rudolph IV Autopol automatic polarimeter at 25 °C (Rudolph, New Jersey, USA). ECD spectra were measured on a JASCO J-810 spectropolarimeter (JASCO Corporation, Tokyo, Japan). IR spectra were recorded on a Thermo Nicolet Nexus 470 FT-IR spectrometer (Thermo, Pennsylvania, USA). NMR spectra were measured on a Bruker Advance 400 and 500 FT NMR spectrometers using TMS as the internal standard (Bruker, Karlsruhe, Germany). HRESIMS spectra were obtained from Xevo G2 Q-TOF mass spectrometer (Waters, Massachusetts, USA). Materials for column chromatography involved silica gel (200–300 mesh, Qingdao Marine Chemical Plant, Qingdao, China), Sephadex LH-20 (18–110 μm, Amersham Pharmacia Biotech AB, Uppsala, Sweden), and ODS gel (50 μm, YMC, Japan). Precoated silica gel plates (Merck, Kieselgel 60 F254, 0.25 mm) were used for TLC analysis. HPLC chromatography was performed on a Waters e2695 Separation Module (Waters, Milford, CT, USA) coupled with a Waters 2998 photodiode array detector (Waters, Milford, CT, USA). A Kromasil C18 semipreparative HPLC column (250 × 10 mm, 5 μm) (EKS Chemicals, Bohus, Sweden) was used for compound purification.
Ods gel
Manufactured by YMC
Sourced in Sweden, China, Japan
ODS gel is a type of laboratory equipment used for chromatographic separation. It consists of silica-based particles coated with octadecylsilane (ODS) groups, which provide a hydrophobic stationary phase for reversed-phase liquid chromatography. The core function of ODS gel is to facilitate the separation and analysis of a wide range of organic compounds, including pharmaceuticals, environmental pollutants, and biological molecules.
Automatically generated - may contain errors
Lab products found in correlation
Nicolet nexus 470 ft ir spectrometer, by Thermo Fisher Scientific (2 mentions)
Sephadex lh 20, by Cytiva (2 mentions)
J 810 spectropolarimeter, by Jasco (1 mentions)
E2695 separation module, by Waters Corporation (1 mentions)
G2 q tof mass spectrometer, by Waters Corporation (1 mentions)
Waters 2998 photodiode array detector, by Waters Corporation (1 mentions)
Prominence lc 20ad, by Shimadzu (1 mentions)
Avance 400 nmr spectrometer, by Bruker (1 mentions)
Ltq orbitrap xl instrument, by Thermo Fisher Scientific (1 mentions)
Hypersil c18 column, by Thermo Fisher Scientific (1 mentions)
Nicolet is5 ftir spectrometer, by Thermo Fisher Scientific (1 mentions)
Gf254 plates, by Qingdao Haiyang Chemical (1 mentions)
Essentia lc 16, by Shimadzu (1 mentions)
Kromasil c18 column, by AkzoNobel (1 mentions)
Avance 3 hd 600, by Bruker (1 mentions)
U 2900 uv vis spectrophotometer, by Hitachi (1 mentions)
Sephadex lh 20 gel, by GE Healthcare (1 mentions)
Mci gel chp 20p, by Mitsubishi Chemical Corporation (1 mentions)
Ft ir 4100 spectrophotometer, by Jasco (1 mentions)
600 mhz spectrometer, by Bruker (1 mentions)
6 protocols using ods gel
1
Analytical Techniques for Natural Product Characterization
2
Spectroscopic Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were measured on a Rudolph IV Autopol automatic polarimeter. IR spectra were determined on a Thermo Nicolet Nexus 470 FT-IR spectrometer. 1D and 2D NMR spectra were recorded on a Bruker Avance 400 NMR spectrometer (400 MHz for 1H and 100 MHz for 13C, respectively). Chemical shifts were referenced to the solvent peaks at δH 2.50 and δC 39.8 for dimethyl sulfoxide (DMSO-d6), respectively, and coupling constants were in Hz. ESIMS and HRESIMS spectra were obtained from a Thermo Scientific LTQ Orbitrap XL instrument. Column chromatography was carried out with silica gel (160–200 mesh, 200–300 mesh), and HF254 silica gel for TLC was obtained from Qingdao Marine Chemistry Co. Ltd. (Qingdao, China), ODS gel (50 μm), and Sephadex LH-20 (18–110 μm) were obtained from YMC (Kyoto, Japan) and Amersham Pharmacia Biotech AB, Uppsala, Sweden. HPLC chromatography was performed on an Alltech instrument (426-HPLC pump), equipped with an Alltech UVIS-200 detector and semipreparative reversed-phase columns (Grace Prevail C18, 5 μm, 250 mm × 10 mm). Analytical HPLC chromatography was performed on Prominence LC-20AD (Shimadzu, Kyoto, Japan) equipped with pump LC-20AD, detector Prominence SPD-M20A PDA and Shimadzu VP-ODS column (150 mm × 4.6 mm), Thermo BDS Hypersil C18 column (150 mm × 4.6 mm, 5 μm).
3
Comprehensive Analytical Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were recorded on a Rudolph Autopol IV-T polarimeter. UV spectra were recorded on a Hitachi U-2900 UV–Vis spectrophotometer. IR spectra were recorded on a ThermoFisher Nicolet iS5 FT-IR spectrometer. NMR spectra were acquired on Varian Mercury Plus 400 instrument and Bruker Avance III HD 600 spectrometer using CD3OD (δH 3.31 and δC 49.0). HRESIMS were obtained on an AB SCIEX 5600+ Q-TOF mass spectrometer. MCI gel CHP-20P (75–150 μm, Mitsubishi Chemical Corporation, Tokyo, Japan), ODS gel (50 μm, YMC Co., Ltd., Japan), and Sephadex LH-20 gel (GE Healthcare Bio-Sciences, USA) were used for column chromatography. Precoated silica gel GF254 plates (Qingdao Haiyang Chemical Co., Ltd., China) were used for TLC analysis. Semi-preparative HPLC was performed on a Shimadzu Essentia LC-16 with a UV detector (210 and 254 nm) and a Kromasil C18 column (150 × 10 mm, 5 μm, AkzoNobel, Co., Sweden).
4
Analytical Methods for Natural Product Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were acquired on a Rudolph Research Analytical Autopol III polarimeter (Rudolph Research Analytical, Hackettstown, NJ, USA). NMR spectra were collected on a Varian Unity 500 MHz (Varian Inc., Palo Alto, CA, USA) and a Bruker 600 MHz spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany). HRESIMS were recorded on Waters Synapt HDMS LC/MS mass spectrometer (Waters Corporation, Milford, MA, USA). IR spectra were measured on a JASCO FT/IR-4100 spectrophotometer (JASCO Corporation, Tokyo, Japan). CD spectra were obtained on a JASCO J-1500 spectrometer (JASCO Corporation, Tokyo Japan). HPLC was performed with PrimeLine Binary pump (Analytical Scientific Instruments, Inc., El Sobrante, CA, USA) and RI-101 (Shoko Scientific Co. Ltd., Yokohama, Japan). Column chromatography was performed using ODS gel (12 nm, S-75 μM, YMC CO., Kyoto, Japan). Semi-preparative HPLC was carried out with an ODS column (YMC-Pack-ODS-A, 250 × 10 mm i.d, 5 μM). Analytical HPLC was performed using an ODS column (YMC-Pack-ODS-A, 250 × 4.6 mm i.d, 5 μM).
5
Identification of Antimicrobial Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Copper sulfate was obtained from Sigma Aldrich, USA. Cation adjusted Mueller–Hinton broth was purchased from Thermo Fisher Scientific, Waltham, MA. Mueller–Hinton (MH) agar was obtained from Merck, Darmstadt, Germany, and the Malt extract (ME) agar was from WINLAB, Leicestershire, UK. The chromatographic gels, diaion HP-20, MCI-gel CHP-20P (Mitsubishi Chemical, Tokyo, Japan), Toyopearl HW-40F (TOSOH, Tokyo, Japan), Sephadex LH-20 (GE Healthcare Bio-Science AB, Uppsala, Sweden), and ODS-gel (YMC, Tokyo, Japan) were used. Dimethyl sulfoxide (DMSO) was procured from Sigma-Aldrich Inc., St. Louis, MO, USA.
6
Spectroscopic Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were determined on a Jasco P-1020 automatic digital polarimeter (Jasco, Tokyo, Japan). CD spectra were recorded on a Chirascan CD spectrometer (Applied Photophysics, Leatherhead, UK). IR spectra were measured on a Jasco FT/IR-4100 spectrometer (Jasco) with KBr pellets. The 1D and 2D NMR spectra were recorded on a Bruker Avance III 500 instruments (Bruker, Fallanden, Switzerland) with TMS as an internal standard. Chemical shifts were reported in units of δ (ppm) and coupling constants (J) were expressed in Hz. Atmospheric pressure chemical ionization MS was determined on a LCQ Fleet ion trap mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA). HR electrospray ionization MS was measured on Agilent G6230 time-of-flight mass spectrometers (Agilent Technologies, Santa Clara, CA, USA). Column chromatography was performed over silica gel (200-300 mesh; Yantai Xinde Chemical Co., Ltd, Yantai, China), ODS gel (YMC, Kyoto, Japan) and Sephadex LH-20 (Amersham Pharmacia Biotech, Uppsala, Sweden). TLC was performed on the silica gel plates (Yantai Xinde Chemical Co., Ltd), and spots were visualized by spraying with 10% H 2 SO 4 in EtOH, followed by heating.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!