According to LPS (CSB-E09945h, CUSABIO, China), IL-1β (CSB-E08053h, CUSABIO, China), TNF-α (CSB-E04740h, CUSABIO, China), and IL-6 (CSB-E04638h, CUSABIO, China) ELISA instructions, we detected LPS level in feces and placental tissues and IL-6, IL-1β, and TNF-α levels in cells. 20 mg of feces and placental tissue was taken, respectively, and the blood stains were washed with 1 × PBS. Feces and placental tissue were cut into small pieces and put into tissue grinder (homogenate tube), 200 μL 1 × PBS was added to make homogenate, then put in -20°C overnight. After repeated freeze-thaw treatment two times to destroy the cell membrane, the tissue homogenate was centrifuged at 5000 g (2-8°C) for 5 min to take the supernatant, which should be detected. For cells, centrifugation was performed at 1000 g (2-8°C) for 15 min, and the supernatant should be immediately detected.
Csb e04740h
Manufactured by Cusabio
Sourced in China, United States
The CSB-E04740h is a laboratory product designed for research purposes. It functions as an instrument used for analytical testing and data collection. Further details about its specific features and capabilities are not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Csb e04638h, by Cusabio (3 mentions)
Csb e08053h, by Cusabio (3 mentions)
Csb e09945h, by Cusabio (1 mentions)
Csb e04593h, by Cusabio (1 mentions)
Csb e04640r, by Cusabio (1 mentions)
Csb e11987r, by Cusabio (1 mentions)
Csb e10684h, by Cusabio (1 mentions)
Ab108837, by Abcam (1 mentions)
Csb e04639m, by Cusabio (1 mentions)
8 protocols using csb e04740h
1
ELISA for Inflammatory Markers in Feces and Tissues
2
Cytokine Profiling in Healthy and CHF
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Blood samples were collected from 20 healthy subjects and 22 CHF patients. The level of IL-6 (CSB-E04638h, Cusabio biotech, China), IL-8 (CSB-E04641h, Cusabio biotech, China), IL-10 (CSB-E04593h, Cusabio biotech, China), and TNF-α (CSB-E04740h, Cusabio biotech, China) in the blood samples was determined by ELISA kit according to the manufacturer's instructions, and all samples analyses were repeated three times.
3
Quantification of CSF IL-1β and TNF-α
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The levels of IL-1β and TNF-α in the CSF of ICI and non-ICI patients were measured using ELISA kits according to the manufacturer’s protocol. Firstly, collected CSF samples were centrifuged. Secondly, 100ul of the supernatants were aspirated. Then, the levels of IL-1β (CUSABIO, CSB-E08053h, USA) and TNF-α (CUSABIO, CSB-E04740h, USA) in supernatants were measured by ELISA kits, respectively. Analysis of optical density was performed in a multifunctional microplate reader.
4
Arecoline Impacts Cell Ultrastructure and Inflammatory Markers
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Following arecoline treatment, cell ultrastructure was observed by transmission electron microscopy. Levels of the inflammatory markers interleukin (IL)-1β (CUSABIO, Cat. CSB-E08053h, Wuhan, China) and tumor necrosis factor (TNF)-α (CUSABIO, Cat. CSB-E04740h, Wuhan, China) in cell culture medium were assessed by enzyme-linked immunosorbent assay.
5
Measurement of Inflammatory Markers in Pregnant Rats
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The concentrations of interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), and sFlt-1 in the cells, cell supernatant, or serum of pregnant rats were measured, according to the manufacturer's instructions. IL-6 (human, CSB-E04638h; rat, CSB-E04640r; Cusabio Biotech, China), TNF-α (human, CSB-E04740h; rat, CSB-E11987r; Cusabio Biotech, China), and sFlt-1 (human, ml038123; rat, ml059017; Mlbio, China) kits were used. Briefly, the samples were pretreated and incubated with an antibody working solution or enzyme at 37 °C for 1 h. Afterward, the samples were incubated with horseradish peroxidase-labeled working solution for 1 h at 37 °C or color developer for 15 min at 37 °C protected from light. Finally, the optical density (OD) value of each well was obtained at 450 nm via a multifunction enzyme analyzer (MB-530, Heales, China).
6
Quantifying Plasma Aβ42 and TNF-α
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ELISA kits #CSB-E10684h and #CSB-E04740h (Cusabio, Houston, TX, USA) were used to assess Aβ42 and TNF-α plasma levels following the manufacturer’s instructions. Datasheets reported kit sensitivity of 0.078 ng/mL and 1.95 pg/mL for Aβ42 and TNFα, respectively. Values below the sensitivity of the kit were excluded from analysis.
7
Multiplex Biomarker Quantification
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Commercially available ELISA kits were used to measure the concentrations of IL-6 (CSB-E04639m, CUSABIO), TNF-α (CSB-E04740h or CSB-E04744m, CUSABIO), I-FABP (CSB-E08024h), D-lactate (HM11235, Bio-Swamp), and ferritin (ab108837, ABCAM) in the indicated samples according to each manufacturer’s instructions.
8
Cytokine and Transcription Factor Quantification
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Whole blood samples were placed at room temperature for 2 h and centrifuged at 2-8°C at 1000 g for 15 min, to collect the supernatant for detection. The supernatant of cell cultures was collected for detection by centrifugation at 2-8°C and 1000 g for 15 min. The cells were washed and transferred to an appropriate centrifuge tube, and 1 × PBS (pH = 7.2-7.4) was added. After the cell membrane was destroyed by ultrasonic crushing, the cells were centrifuged at 2-8°C and 5000 g for 5 min to remove the supernatant for detection. Monocyte chemoattractant protein-1 (MCP-1) (CSB-E04655H, CUSABIO), tumour necrosis factor alpha (TNF-a) (CSB-E04740H, CUSABIO), and KLF4 (JL51834, Shanghai Jianglai Biotechnology Co., Ltd) and a Multifunctional Enzyme Marker Analyzer (MB-530, HEALES) were used to analyse MCP-1, TNF-a, and KLF4 levels in blood, cell supernatant, and cells, respectively.
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