Cutaneous wound healing assays were carried out as previously described (Tyner et al., 2002 (link)). In brief, mice were anesthetized and a full-thickness wound was generated in the mouse dorsal skin using a 3 mm biopsy punch (Integra, York, PA). Wound diameters were measured daily. Wound areas = 0.25 × π ×width × length.
3 mm biopsy punch
Manufactured by Integra LifeSciences
The 3 mm biopsy punch is a medical device used to obtain a small sample of tissue from the body. It is a circular cutting instrument with a diameter of 3 millimeters.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using 3 mm biopsy punch
1
Cutaneous Wound Healing Assay
2
Surgical Implantation of Cranial Window
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animals were anesthetized using the fentanyl cocktail (i.p.) during the cranial window implantation surgical procedure. Body temperature was maintained at 37°C with a heating pad and the animal’s eyes were protected with lubricant ointment. All surgical procedures adhered to aseptic technique. Mice were fixed in a stereotaxic frame; hair was removed, and the skull was exposed through a scalp incision. A 3-mm biopsy punch (Integra) was then used to create a circular score on the skull over V1. A 0.5-mm drill bit (FST) was used to then drill through the skull for the craniotomy, tracing the 3-mm score. A 5-mm coverslip attached to a 3-mm coverslip (Warner Instruments) by UV glue (Norland Optical Adhesive, Norland) was then slowly lowered into the craniotomy (3-mm side down). The coverslip was carefully secured with C&B Metabond dental cement (Parkell). A custom headplate produced by emachine shop (http://www.emachineshop.com ) (designs courtesy of the Mriganka Sur laboratory, Massachusetts Institute of Technology) was then secured onto the skull with the same dental cement, the rest of which was used to cover any exposed skull and seal the incision site. Mice were administered slow-release buprenex (5 mg/kg subcutaneously for 72 h) and carprofen (5 mg/kg, i.p. every 24 h) and monitored for 72 h postoperatively.
3
Mouse Craniotomy for Neuroimaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All surgeries were performed on mice anesthetized using ketamine (100 mg/kg, i.p.) and xylazine (10 mg/kg, i.p.). Anesthetized mice were placed in a stereotaxic frame (catalog #514, Kopf Instruments), and injections of an anti-inflammatory drug (carprofen, 5 mg/kg, s.c.) and a local anesthetic (lidocaine, s.c.) were administered. A craniotomy was performed over the right primary AC (anteroposterior, −3.1 mm; mediolateral, 4.6 mm; lateral from midline; Extended Data Fig. 1-1 ) using a 3 mm biopsy punch (Integra), and a 3-mm-diameter round glass cranial window was secured over this craniotomy. A custom-made lightweight (<1 g) titanium head bar was attached to the left side of the skull using dental cement and cyanoacrylate glue to allow for head-fixed imaging. During the surgery, body temperature was maintained at 38°C, and the depth of anesthesia was regularly assessed by checking the pinch withdrawal reflex. Mice were treated with carprofen for 48 h postsurgically and allowed to recover for a week.
4
Cranial Window Implantation for Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animals were anesthetized using the fentanyl cocktail (i.p.) during the cranial window implantation surgical procedure. Body temperature was maintained at 37°C with a heating pad and the animal’s eyes were protected with lubricant ointment. All conducted surgical procedures adhered to aseptic technique. Mice were fixed in a stereotaxic frame; hair was removed and the skull was exposed through a scalp incision. A 3-mm biopsy punch (Integra) was then used to create a circular score on the skull over V1. A 0.5-mm drill bit (FST) was used to then drill through the skull for the craniotomy, tracing the 3-mm score. A 5-mm coverslip attached to a 3-mm coverslip (Warner Instruments) by UV glue (Norland Optical Adhesive, Norland) was then slowly lowered into the craniotomy (3-mm side down). The coverslip was carefully secured with C&B Metabond dental cement (Parkell). A custom headplate produced by emachine shop (http://www.emachineshop.com ) (designs courtesy of the Mriganka Sur laboratory, Massachusetts Institute of Technology) was then secured onto the skull with the same dental cement, the rest of which was used to cover any exposed skull and seal the incision site. Mice were administered slow-release buprenex (5 mg/kg subcutaneously for 72 h) and carprofen (5 mg/kg, i.p. every 24 h) and monitored for 72 h postoperatively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!