Log In Sign up
The largest database of trusted experimental protocols

Helios fitc 22f6

Manufactured by BioLegend
Visit Supplier

HELIOS-FITC (22F6) is a fluorescently-labeled antibody that can be used for flow cytometry analysis. The FITC fluorophore is conjugated to the 22F6 clone of the HELIOS antibody, which targets a specific cell surface marker. This product provides researchers with a tool to identify and quantify cells expressing the target antigen using flow cytometry techniques.

Automatically generated - may contain errors

Lab products found in correlation

Cd27 bv605, by Thermo Fisher Scientific (1 mentions) Cd38 bv785, by Thermo Fisher Scientific (1 mentions) Cd20 bv570, by Thermo Fisher Scientific (1 mentions) Igd pe, by Southern Biotech (1 mentions) Cd19 apc, by Thermo Fisher Scientific (1 mentions) Cd4 apc cy7 rm4 5, by BioLegend (1 mentions) Cd25 pe cy7 pc61, by BioLegend (1 mentions) Facscanto 2 system, by BD (1 mentions) Foxp3 staining kit, by Thermo Fisher Scientific (1 mentions) Foxp3 apc fjk 16s, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Helios (22F6; (8 citations) Helios-FITC (3 citations) Helios-FITC (clone 22F6) (2 citations)

2 protocols using helios fitc 22f6

1

Polychromatic Flow Cytometry Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
polychromatic flow cytometry was performed using the following directly conjugate antibodies: (1) BD Biosciences: CD3-H7APC (SK7), BCL-6-PE (K112–91), CD134 (OX40)-BV650 (ACT35), IgM-Cy5PE (G20–127), IgG-Cy5PE (G18–145), CD210 (IL-10R)-PE (3F9), IgG-APC (G18–145), CD19-APC (HIB19), CD95-BV421 (DX2); (2) eBioscience: HELIOS-FITC (22F6) (3) Biolegend: CD185 (CXCR5)-FITC (J252D4), CD150 (SLAM)-PE (A12–7D4), CD279 (PD-1)-BV711 (EH12.2H7), CD20-BV570 (2H7), CD278 (ICOS)-PB (C398.4A), Ki67-Alexa700 (Ki-67), FOXP3-PB (206D), CD27-BV605 (O323), CD38-BV785 (HIT2), CD124 (IL-4R)-Cy7PE (G077F6), CCR7-BV605 (G043H7); (4) Invitrogen: CD4-Cy5.5PE (S3.5); (5) Beckman Coulter: CD19-ECD, CD45-ECD, CD27-PC5; (6) R&D Systems: TGF-beta RII- FLUORESCIN; (7) SouthernBiotech: IgD-PE. CD57 (NK1) antibody was conjugated in-house.
Amodio D., Cotugno N., Macchiarulo G., Rocca S., Dimopoulos Y., Castrucci M.R., De Vito R., Tucci F.M., McDermott A.B., Narpala S.R., Rossi P., Koup R.A., Palma P, & Petrovas C. (2017). Quantitative multiplexed imaging analysis reveals a strong association between immunogen-specific B cell responses and tonsillar germinal center immune dynamics in children after influenza vaccination. Journal of immunology (Baltimore, Md. : 1950), 200(2), 538-550.
+ Open protocol
+ Expand
2

Multiparameter Flow Cytometry for T Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
For analysis of T cell subsets, cells were stained with CD3-PerCp-Cy5 (17A2; BioLegend, San Diego, CA), CD4-APC-Cy7 (RM4-5; BioLegend, San Diego, CA), neuropilin-1-PE (761705; R&D Systems, Minneapolis, MN), helios-FITC (22F6; BioLegend, San Diego, CA), CD25-Bio (7D4), CD25-PE-Cy7 (PC61; BioLegend, San Diego, CA) and FoxP3-APC (FJK-16s; eBioscience, San Diego, CA). Intracellular staining was performed with a FoxP3 staining kit (eBioscience), as described previously ). Flow cytometry was performed on a BD FACSCanto II system (BD Biosciences, San Jose, CA), and data were analyzed by FlowJo (10.0.8) software (FlowJo LLC, Ashland, OR).
Schwarz C., Unger L., Mahr B., Aumayr K., Regele H., Farkas A.M., Hock K., Pilat N, & Wekerle T. (2016). The Immunosuppressive Effect of CTLA4 Immunoglobulin Is Dependent on Regulatory T Cells at Low But Not High Doses. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 16(12).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!