Ddx17

Manufactured by Santa Cruz Biotechnology

Sourced in United States

DDX17 is a protein that functions as an RNA helicase, which is an enzyme that unwinds and separates double-stranded RNA molecules. This activity is important for various cellular processes, such as RNA metabolism, transcription, and RNA splicing.

Automatically generated - may contain errors

Lab products found in correlation

Snrpa, by Santa Cruz Biotechnology (1 mentions) Fibrillarin, by Cell Signaling Technology (1 mentions) Tdp 43, by Proteintech (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Cx 5461, by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Phospho jnk, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Rnase a, by Fujifilm (1 mentions) Ddx21, by Santa Cruz Biotechnology (1 mentions) Bcl xl, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-DDX17 (2 citations)

2 protocols using ddx17

Monoclonal and Polyclonal Antibodies for RNAP II

Check if the same lab product or an alternative is used in the 5 most similar protocols

The monoclonal antibodies used in this study were 8WG16 (against POLR2A, the large subunit of RNAP II) from Biolegend (cat # 920102), SNRPC from Sigma (cat # SAB4200188), DHX9 from Abcam (cat # ab26271), SNRPA (cat # sc-101149), DDX5 (cat # sc-166167), DDX17 (cat # sc-86409) from Santa Cruz. The polyclonal antibodies were FUS (cat # A300–293A), EWSR1 (cat # A300–418A) and MATR3 (cat # A300–591A) from Bethyl, TAF15 from Novus (cat # NB100–567), DDX17 (cat # sc-86409) from Santa Cruz, and DHX9 (cat # ab 26271) from Abcam. Our rabbit polyclonal antibodies to THOC2, EIF4A3, and DDX39B have been described^{58 (link),59 (link)}.

Chi B., O’Connell J.D., Yamazaki T., Gangopadhyay J., Gygi S.P, & Reed R. (2018). Interactome analyses revealed that the U1 snRNP machinery overlaps extensively with the RNAP II machinery and contains multiple ALS/SMA-causative proteins. Scientific Reports, 8, 8755.

+ Open protocol

+ Expand

Antibody Validation and Reagent Sourcing

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were purchased from suppliers: Bcl-xL (#sc-634), CHOP (#sc-793), DDX5 (#sc-365164), DDX17 (#sc-398168), DDX21 (#sc-376758), GST (#sc-138), and JNK1 (#sc-571), Santa Cruz Biotechnology (Dallas, TX, USA); Bim (#2933), Cleaved Caspase-3 (#9661), Fibrillarin (#2639), GAPDH (#2118), and phospho-JNK (#9255), Cell Signaling Technology (Danvers, MA, USA); FLAG (#F1804) and horseradish peroxidase (HRP)-conjugated FLAG (#A8592), Sigma-Aldrich (St. Louis, MO, USA); TDP-43 (#12892-1-AP), Proteintech Group (Rosemont, IL, USA); HA (#11867423001) and HRP-conjugated HA (#12013819001), Roche Diagnostics (Basel, Swiss); DDX18 (#A300-636A), Bethyl Laboratories (Montgomery, TX, USA); Myc (#BML-SA294-0500), Enzo Life Sciences (Farmingdale, NY, USA). AS601245 and CX5461 were purchased from Merck Millipore (Burlington, MA, USA) and AdooQ BioScience (Irvine, CA, USA), respectively. RNase A were purchased from Wako (Osaka, Japan).

Suzuki H., Shibagaki Y., Hattori S, & Matsuoka M. (2018). The proline–arginine repeat protein linked to C9-ALS/FTD causes neuronal toxicity by inhibiting the DEAD-box RNA helicase-mediated ribosome biogenesis. Cell Death & Disease, 9(10), 975.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!