Female NOD/SCID mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China), and housed under specific pathogen‐free condition. All animal experiments were approved by the Institutional Animal Care and Use Committee of the Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences. 6 × 106 Karpas299 cells suspended in 200 μL PBS were injected subcutaneously into the right flank of 6‐ to 8‐week‐old mice. When the tumor volume reached 100 mm3, mice were randomized into six groups (n = 6 per group) and treated intravenously with various doses of anti‐CD30‐LDM, or 0.6 mg·kg−1 anti‐CD30 antibody, or 0.045 mg·kg−1 LDM, respectively, every 7 days for a total of two injections. The control group was given PBS only. Tumors were measured twice a week with a caliper, and tumor volumes were determined using the formula: (length × width2)/2. The inhibition rate of tumor growth was calculated as [1 − (tumor volumetreated final − tumor volumetreated initial)/(tumor volumecontrol final − tumor volumecontrol initial)] × 100%. At the end of the experiment, mice were euthanized. Subcutaneous tumors and various organs were harvested and fixed in 10% formalin for hematoxylin and eosin (H&E) staining and immunohistochemical analysis.
Female nod scid mice
Manufactured by Charles River Laboratories
Sourced in China, Japan, Italy, United States
Female NOD/SCID mice are a strain of immunodeficient rodents developed for use in biomedical research. These mice lack functional T cells, B cells, and natural killer cells, making them useful for the study of human cell and tissue engraftment.
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Lab products found in correlation
Matrigel, by BD (5 mentions)
Matrigel basement membrane matrix, by Corning (2 mentions)
Micropet focus 120, by Siemens (1 mentions)
Nor noha, by Cayman Chemical (1 mentions)
Lamivudine lam, by GlaxoSmithKline (1 mentions)
Hi fbs, by Thermo Fisher Scientific (1 mentions)
Cb17 scid female mice, by Charles River Laboratories (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
C57bl 6j mice, by GemPharmatech (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
Male balb c mice, by Charles River Laboratories (1 mentions)
Mmtv cre line d, by Jackson ImmunoResearch (1 mentions)
K14 cre mice, by Jackson ImmunoResearch (1 mentions)
Matrigel matrix, by Corning (1 mentions)
Nod cb17 alhnrj prkdcscid rj mice, by Janvier Labs (1 mentions)
Forane, by Abbott (1 mentions)
Matrigel basement membrane matrix, by BD (1 mentions)
53 protocols using female nod scid mice
1
In Vivo Evaluation of Anti-CD30 Antibody-Drug Conjugate for Lymphoma Treatment
2
Breast Cancer Metastasis Modulation
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Female NOD/SCID mice, 6 to 8 weeks of age, were purchased from Vital River Laboratories. Approximately 1 × 106 of luciferase-transfected MDA-MB-231 cells, mixed with Matrigel (1:1), were transplanted into 4th mammary gland fat pad of NOD/SCID mice. Mice were randomly divided into experimental groups with 5 mice in each group. After 12 days, 1 nmol of microRNA agomir (Ribobio, China) in 20 μl PBS was infused into the mammary gland once every 3 days for a total of 12 days (four times). Primary and metastatic tumors in mice were detected by PET scan (MicroPET Focus 120, Siemens, Germany). Lung tissues of each mice were fixed in formalin and embedded in paraffin for histologic analysis. There were four mice in each group in subsequent experiments.
3
Humanized Lupus Mouse Model
4
Murine Xenograft Model Establishment
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Female NOD/SCID mice at 4–5 weeks of age were purchased from Beijing Vital River Laboratory Animal Technology (Beijing, China). Mice were housed in pressurized, individually ventilated cages (PIV/IVC) and maintained under specific-pathogen-free conditions, with free access to food and water in a 12 h light/dark cycle. All animal studies were approved by the Animal Care and Use Committee of Sichuan University.
5
Evaluation of Lamivudine and Entecavir in NOD/SCID Mice
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Female NOD/SCID mice at 6–9 weeks of age were purchased from Vital River Laboratory Animal Technology Co., Ltd., Beijing, China. Animals were bred and cared under specific pathogen-free conditions in the Experimental Animal Center of the Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences. Our studies on mice were carried out in strict accordance with the recommendations in the Code of Ethics of the World Medical Association.
Lamivudine (LAM) and entecavir (ETV) tablets were purchased from GlaxoSmithKline Pharmaceuticals (Suzhou) Co., Ltd. and Sino-American Shanghai Squibb Pharmaceuticals Co., Ltd., respectively.
Lamivudine (LAM) and entecavir (ETV) tablets were purchased from GlaxoSmithKline Pharmaceuticals (Suzhou) Co., Ltd. and Sino-American Shanghai Squibb Pharmaceuticals Co., Ltd., respectively.
6
NOD-SCID Mouse Maintenance Protocol
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Female NOD-SCID mice, aged four to six weeks, weighed 18–20 g, were purchased from Beijing Vital River Laboratory Animal Technology (Beijing, China). The NOD-SCID mice were housed and maintained in isolator cages under a specific-pathogen-free (SPF) environment with a controlled humidity and temperature, on a standard 12 h light/dark cycle, at Wenzhou Medical University. All animal care and experimental procedures complied with the guidelines for ethical review of animal welfare and were approved by the Institutional Animal Care and Use Committee of Wenzhou Medical University.
7
Xenograft Leukemia Model in NOD/SCID Mice
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Female NOD/SCID mice (3–4 weeks of age) were obtained from Beijing Vital River Laboratory Animal Technology Co., Ltd (Beijing, China). All animal protocols were performed in accordance with the guidelines of the institutional animal ethics committee and were permitted by the Institutional Animal Committee of Nankai University (SYXK 2014-0003, No. 94 Weijin Road, Nankai District, Tianjin, China). All the mice were housed in a specific pathogen-free environment under a 12-h light/12-h dark cycle. The mice were randomly divided into the control group and 20(S)-GRh2 group. All mice were treated with cyclophosphamide (100 mg/kg) for two days by intraperitoneal injection. Twenty-four hours later, Jurkat cells (5.0 × 106 cells, 200 μL volume) were administrated into the mice via tail vein injection. Once ≥1% leukemic cells were detected in the peripheral blood, 20(S)-GRh2 (40 mg/kg body weight (b.w.)) was administered once daily for three consecutive weeks by oral gavage in the 20(S)-GRh2 group. The control group received 0.1% DMSO. Body weights were measured twice a week. On Day 22, the mice were sacrificed, and the blood was collected for routine examination. The spleens were harvested for immunohistochemistry analysis.
8
Mouse Xenograft Implantation Protocol
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Female NOD SCID mice were purchased from the Beijing Vital River Laboratory Animal Technology Co., Ltd and were between 6-8 weeks of age at the time of implantation. Mice were hosted in a specific pathogen-free (SPF) environment of a vivarium facility and acclimatized to their new environment for at least three days before the initiation of any experiments following IACUC protocols.
9
Cell Lines and Animal Model for Cancer Research
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The human breast cancer cell lines MCF7, SKOV3, and SKBR3; the colon cancer cell line LS174T; and the Chinese hamster ovary cell line CHO were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). MCF7, SKBR-3, and SKOV3 cells were cultured in DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, Thermo Fisher Scientific) and 1% penicillin/streptomycin. LS174T and CHO cells were cultured in RPMI-1640 medium (Gibco, Thermo Fisher Scientific) with 10% HI-FBS (Gibco, Thermo Fisher Scientific) and 1% penicillin/streptomycin. All cells were incubated at 37°C in a humidified incubator with 5% CO2.
Female NOD/SCID mice and female CB-17 SCID mice were purchased from Vital River Laboratory Animal Technology (Beijing, China). The animals were acclimated for 1 week prior to experiment. The mice were housed in a temperature- and humidity-controlled environment with a controlled light-dark cycle (12-12 h). Animal care and experimental procedures were approved by the Institutional Animal Care and Use Committee, Sun Yat-Sen University.
Female NOD/SCID mice and female CB-17 SCID mice were purchased from Vital River Laboratory Animal Technology (Beijing, China). The animals were acclimated for 1 week prior to experiment. The mice were housed in a temperature- and humidity-controlled environment with a controlled light-dark cycle (12-12 h). Animal care and experimental procedures were approved by the Institutional Animal Care and Use Committee, Sun Yat-Sen University.
10
Evaluation of Icaritin Effects on Multiple Myeloma
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Female NOD/SCID mice (6-week old) were purchased from Vital River Laboratories (Beijing, China) and maintained under conventional conditions. All animal work was approved by the Institutional Review Board of the second Xiangya hospital, Central south University. U266 cells (2 × 107 cells) were injected into each mouse by subcutaneously inoculation in the right flank area. After tumors volume grew to 50 mm3, the mice were administered icaritin (3 mg/kg or 6 mg/kg) or bortezomib (as positive control; 0.75 mg/Kg) every 2–3 day with intraperitoneal injection (i.p). Tumor growth and mice body weight were monitored every other day for 21days. Tumor volume was calculated using the formula: V = 0.5 × a × b2, where a and b represented the long and short diameter of the tumor, respectively. At the twenty-first day, all mice were sacrificed individually by cervical dislocation and blood was collected for ELISA. Tumor xenografts were removed, weighed, incised and pathological sections were prepared and stained with immunohistochemistry.
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